Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 233454..234096 Replicon chromosome
Accession NZ_CP004858
Organism Bacillus thuringiensis serovar kurstaki str. YBT-1520

Toxin (Protein)


Gene name pemK Uniprot ID R8I8B8
Locus tag YBT1520_RS01315 Protein ID WP_000635965.1
Coordinates 233746..234096 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8I8H2
Locus tag YBT1520_RS01310 Protein ID WP_000004570.1
Coordinates 233454..233741 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
YBT1520_RS01285 228771..229733 + 963 WP_000961167.1 UV DNA damage repair endonuclease UvsE -
YBT1520_RS01290 229726..230298 - 573 WP_000908522.1 rhomboid family intramembrane serine protease -
YBT1520_RS01295 230392..230751 + 360 WP_000583417.1 holo-ACP synthase -
YBT1520_RS01300 230908..231858 + 951 WP_002100659.1 outer membrane lipoprotein carrier protein LolA -
YBT1520_RS01305 231976..233145 + 1170 WP_000390616.1 alanine racemase -
YBT1520_RS01310 233454..233741 + 288 WP_000004570.1 antitoxin EndoAI Antitoxin
YBT1520_RS01315 233746..234096 + 351 WP_000635965.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
YBT1520_RS01320 234164..236332 + 2169 WP_000426236.1 RNA-binding transcriptional accessory protein -
YBT1520_RS01325 236391..236507 - 117 WP_001143642.1 cortex morphogenetic protein CmpA -
YBT1520_RS01330 236703..237161 + 459 WP_000344239.1 SprT family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12948.04 Da        Isoelectric Point: 5.7168

>T45043 WP_000635965.1 NZ_CP004858:233746-234096 [Bacillus thuringiensis serovar kurstaki str. YBT-1520]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMSRVDEALQISLGLIDF

Download         Length: 351 bp

>T45043 NZ_CP004858:233746-234096 [Bacillus thuringiensis serovar kurstaki str. YBT-1520]
TTGATTGTAAAACGCGGCGACGTGTATTTTGCAGACCTTTCCCCAGTTGTTGGTTCTGAGCAAGGAGGCGTTCGTCCGGT
TCTTGTCATTCAAAATGACATCGGAAATCGTTTTAGTCCAACGGTGATTGTAGCGGCTATTACTGCACAGATTCAAAAAG
CGAAATTACCCACTCATGTGGAAATTGATGCGAAAAAGTATGGTTTTGAGAGAGATTCTGTTATTTTACTTGAGCAGATT
CGAACAATCGATAAGCAACGCTTAACGGACAAAATCACTCATTTGGATGAAGTGATGATGAGTCGTGTAGATGAAGCGTT
ACAAATTAGTTTAGGACTAATAGATTTTTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10780.36 Da        Isoelectric Point: 8.9605

>AT45043 WP_000004570.1 NZ_CP004858:233454-233741 [Bacillus thuringiensis serovar kurstaki str. YBT-1520]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp

>AT45043 NZ_CP004858:233454-233741 [Bacillus thuringiensis serovar kurstaki str. YBT-1520]
GTGTCCGAATCAAGTGTAACTACTGAAATCGTGGTTCGGTTGCCAAAGCAAATGGTAACGGAATTGGACGGAATTGGAAA
ACAAGAGAATAAGAATCGCCATGAACTAATTTGCCAGGCAACACAATTGTTATTGCGTCAACATAAGACGAAGAAACGCT
ACCAACATGAATCAATGCGACGTGGGTACATTGAAATGGGAAAAATTAATCTTGGTATTGCATCTGAAGCTTTCTTAGCA
GAGTATGAAGCAGCTCATACAGTAGAACGCTTAGTTAGCGGGGGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A366G118

Antitoxin

Source ID Structure
AlphaFold DB A0A366FY90

References