Detailed information of TA system TA045030

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-couple_hipB
• Location: 2371155..2372932
• Replicon: chromosome
• Accession: NZ_CP004387
• Organism: Alcanivorax pacificus W11-5

Toxin (Protein)

• Gene name: HipA3
• Uniprot ID: A0A0B4XN94
• Locus tag: S7S_RS10750
• Protein ID: WP_008735091.1
• Coordinates: 2371598..2372932 (+)
• Length: 445 a.a.

Antitoxin (Protein)

• Gene name: HipB3
• Uniprot ID: -
• Locus tag: S7S_RS10745
• Protein ID: WP_008735092.1
• Coordinates: 2371155..2371496 (+)
• Length: 114 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
S7S_RS10730	2367310..2367738	-	429	WP_008735095.1	low molecular weight phosphotyrosine protein phosphatase	-
S7S_RS10735	2367738..2368898	-	1161	WP_052269242.1	polysaccharide biosynthesis/export family protein	-
S7S_RS10740	2368921..2370414	-	1494	WP_035203867.1	capsule assembly Wzi family protein	-
S7S_RS10745	2371155..2371496	+	342	WP_008735092.1	helix-turn-helix domain-containing protein	Antitoxin
S7S_RS10750	2371598..2372932	+	1335	WP_008735091.1	type II toxin-antitoxin system HipA family toxin	Toxin
S7S_RS10755	2372984..2373463	-	480	WP_008735086.1	GNAT family N-acetyltransferase	-
S7S_RS10760	2373465..2373794	-	330	WP_008735085.1	DUF1778 domain-containing protein	-
S7S_RS10765	2373989..2374285	-	297	WP_008735084.1	helix-hairpin-helix domain-containing protein	-
S7S_RS10770	2374382..2375077	-	696	WP_008735082.1	orotidine-5'-phosphate decarboxylase	-
S7S_RS10775	2375126..2376280	-	1155	WP_008735080.1	lipopolysaccharide assembly protein LapB	-
S7S_RS10780	2376283..2376522	-	240	WP_008735078.1	hypothetical protein	-
S7S_RS10785	2376537..2376905	-	369	WP_082027799.1	integration host factor subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (14-124)

Antitoxin

• Cro/C1-type helix-turn-helix domain (16-66)

Sequences

Toxin

Protein

• Length: 445 a.a.
• Molecular weight: 49492.04 Da
• Isoelectric Point: 6.7697

>T45030 WP_008735091.1 NZ_CP004387:2371598-2372932 [Alcanivorax pacificus W11-5]
MKKGTTQGGTLVASVVLWGAQIGAVRWDTERQLGFFEYAPAFRRSGIQVAPLTMPLDNSIHAFPQLARDTFRGLPGLLAD
SLPDKFGNAVIDAWLAEQGRSREDFSPVERLCYIGRRGMGALEFEPAAGRGTGNALPLDIGALAALSDAILAQRDGFSTW
LSEDEQRNEKAMRDIIQVGVSAGGARAKALVAWNETTQEIRSGQLQAPPGFSHWLLKFDGVTHNRDKERFDPAGFGLIEY
AYYLMATAAGIDMPECRLLRENGRSHFMCRRFDRTDDGQKLHMQSLCAIAHYDFNMAGAWSYEQALQVMQRLGLSKLALE
EQFRRMVFNVLARNQDDHTKNIAFLMNRRGEWSLSPAFDVTFAYNPDGAWTQQHQMSIQGKRDHFSRDDLLAVARRFAIM
DKRALDIVDGVATAIRQWPAFAAEAGVDEATTQAIAAAQAQRQW

Nucleotide

• Length: 1335 bp

>T45030 NZ_CP004387:2371598-2372932 [Alcanivorax pacificus W11-5]
ATGAAAAAAGGAACGACACAAGGCGGCACACTGGTGGCGAGCGTGGTGCTGTGGGGTGCGCAGATTGGCGCGGTGCGCTG
GGACACTGAGCGGCAACTCGGCTTTTTTGAGTATGCGCCGGCGTTTCGGCGTAGCGGCATCCAGGTGGCACCGCTGACCA
TGCCACTGGACAACAGCATCCATGCTTTCCCGCAGCTGGCACGCGACACCTTTCGCGGCCTGCCCGGCCTGCTGGCCGAT
TCGCTACCGGACAAATTCGGCAACGCGGTAATTGATGCCTGGCTGGCGGAACAGGGTCGCTCGCGCGAGGACTTCAGCCC
GGTGGAACGGCTCTGTTATATCGGCCGTCGCGGCATGGGCGCGCTGGAATTCGAACCGGCAGCCGGTCGGGGCACCGGTA
ATGCGCTGCCACTGGATATCGGTGCCCTGGCGGCACTGTCCGATGCCATTCTCGCGCAGCGTGACGGCTTCAGCACCTGG
CTTTCTGAAGACGAACAGCGCAATGAGAAAGCCATGCGCGACATCATCCAGGTCGGTGTTTCTGCTGGCGGTGCCCGCGC
CAAGGCACTGGTGGCATGGAACGAAACCACGCAGGAGATCCGCTCCGGGCAATTACAGGCCCCGCCCGGTTTCAGTCACT
GGCTGCTGAAGTTCGATGGCGTCACGCATAATCGGGACAAGGAGCGGTTCGATCCGGCAGGCTTCGGCCTGATCGAATAC
GCCTACTACCTGATGGCCACCGCCGCCGGCATCGATATGCCGGAATGCCGGCTGCTGCGCGAAAATGGCCGCAGTCACTT
CATGTGCCGCCGCTTTGATCGCACCGATGACGGACAGAAGCTGCACATGCAATCGCTGTGTGCGATCGCCCATTACGATT
TCAATATGGCCGGCGCCTGGTCCTACGAGCAGGCCCTGCAGGTAATGCAACGCCTGGGGCTGAGCAAGCTGGCACTGGAA
GAGCAATTTCGCCGCATGGTGTTCAATGTGCTGGCACGCAACCAGGATGACCACACCAAAAACATTGCCTTCCTGATGAA
CCGGCGCGGTGAATGGTCGCTCTCCCCGGCCTTCGATGTGACCTTCGCCTACAACCCGGACGGCGCCTGGACCCAGCAGC
ACCAGATGAGCATCCAGGGGAAGCGGGATCACTTCAGCCGTGACGACCTGCTCGCCGTGGCACGACGTTTCGCGATCATG
GACAAGCGGGCGCTGGACATTGTCGACGGCGTTGCCACCGCGATCCGGCAGTGGCCGGCTTTCGCAGCAGAGGCCGGTGT
GGATGAGGCCACCACACAGGCCATTGCGGCGGCGCAGGCGCAGCGGCAGTGGTGA

Antitoxin

Protein

• Length: 114 a.a.
• Molecular weight: 12681.29 Da
• Isoelectric Point: 4.9265

>AT45030 WP_008735092.1 NZ_CP004387:2371155-2371496 [Alcanivorax pacificus W11-5]
MDYLGTSDDVILAELGQRVARLRLNRNLKQDELALEAGISRKTLSRLENGHPVDTVNLIRVLRALGQLDQLDALLPPAEI
SPVDLARLQGRRRQRASGVREDSSDTGDWEWPE

Nucleotide

• Length: 342 bp

>AT45030 NZ_CP004387:2371155-2371496 [Alcanivorax pacificus W11-5]
ATGGATTATCTGGGCACAAGCGATGATGTCATCCTCGCGGAGCTGGGCCAGCGCGTTGCCCGCCTGCGCCTCAATCGCAA
TCTCAAGCAGGATGAGTTGGCCCTGGAGGCCGGCATTTCCCGCAAGACATTGTCCCGGCTGGAGAATGGCCACCCCGTGG
ACACCGTCAATCTCATCCGGGTACTGCGTGCACTCGGCCAGCTGGATCAACTGGATGCCCTGCTGCCGCCGGCAGAAATC
AGCCCTGTGGACCTGGCACGCCTGCAGGGTCGCCGACGGCAACGCGCCAGCGGTGTGCGTGAAGACAGCAGTGACACGGG
CGATTGGGAATGGCCGGAATGA

Structures

Toxin

• AlphaFold DB: A0A0B4XN94