Detailed information of TA system TA045027

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 556392..557023
• Replicon: chromosome
• Accession: NZ_CP004387
• Organism: Alcanivorax pacificus W11-5

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: S7S_RS02590
• Protein ID: WP_008739687.1
• Coordinates: 556392..556790 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A0B4XG05
• Locus tag: S7S_RS02595
• Protein ID: WP_008739688.1
• Coordinates: 556787..557023 (-)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
S7S_RS02560	551840..552061	-	222	WP_008739681.1	hypothetical protein	-
S7S_RS02565	552054..552431	-	378	WP_008739682.1	hypothetical protein	-
S7S_RS02570	552421..553749	-	1329	WP_008739683.1	hypothetical protein	-
S7S_RS02575	553758..554027	-	270	WP_008739684.1	WXG100 family type VII secretion target	-
S7S_RS02580	554049..554759	-	711	WP_041025922.1	VWA domain-containing protein	-
S7S_RS02585	554979..555992	-	1014	WP_008739686.1	WYL domain-containing protein	-
S7S_RS02590	556392..556790	-	399	WP_008739687.1	tRNA(fMet)-specific endonuclease VapC	Toxin
S7S_RS02595	556787..557023	-	237	WP_008739688.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
S7S_RS02600	557225..558511	-	1287	WP_008739689.1	glutamate-1-semialdehyde 2,1-aminomutase	-
S7S_RS02605	558579..559196	-	618	WP_008739694.1	thiamine phosphate synthase	-
S7S_RS02610	559193..559954	-	762	WP_008739705.1	hydroxymethylpyrimidine/phosphomethylpyrimidine kinase	-
S7S_RS02615	560160..560579	-	420	WP_008739706.1	protoporphyrinogen oxidase HemJ	-
S7S_RS02620	560699..561757	+	1059	WP_008739707.1	N-acetyl-gamma-glutamyl-phosphate reductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-124)

Antitoxin

• SpoVT-AbrB domain (7-51)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14876.86 Da
• Isoelectric Point: 7.3567

>T45027 WP_008739687.1 NZ_CP004387:c556790-556392 [Alcanivorax pacificus W11-5]
MIRYMLDTNIVIYTLNNRPRHVRGRFIAHDGQMCVSSVTMMELYFGAEKSNNPGRNIAEIEQFAARLDVFDYDTEAAAHT
GQIRNELRLSGKNIGPYDQMIAGHARSAGLIVITNNVSEFSRVSGLRVENWA

Nucleotide

• Length: 399 bp

>T45027 NZ_CP004387:c556790-556392 [Alcanivorax pacificus W11-5]
ATGATCCGGTATATGCTGGATACCAACATCGTGATCTACACCCTGAACAACCGTCCTCGCCACGTTCGGGGCCGCTTCAT
TGCTCACGATGGCCAAATGTGCGTGTCTTCCGTGACGATGATGGAACTCTATTTCGGCGCGGAAAAGTCCAATAACCCTG
GCCGCAATATCGCAGAAATCGAACAGTTTGCCGCCCGCCTGGATGTTTTTGACTACGACACCGAAGCGGCCGCGCATACC
GGCCAGATCCGCAACGAGCTGAGACTCTCAGGAAAAAACATAGGCCCGTACGACCAGATGATTGCTGGCCACGCCCGTTC
CGCCGGGCTCATCGTCATCACCAATAACGTCAGTGAATTCAGCCGCGTATCAGGATTGCGAGTCGAAAACTGGGCCTGA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 9132.22 Da
• Isoelectric Point: 4.4746

>AT45027 WP_008739688.1 NZ_CP004387:c557023-556787 [Alcanivorax pacificus W11-5]
MERVRIFKNNQSRAIRLPKALDFSLDVQEVDIIRVGNTRIIVPAGQGWATWFDRDARVTDDFMAEREQPDDQERDALE

Nucleotide

• Length: 237 bp

>AT45027 NZ_CP004387:c557023-556787 [Alcanivorax pacificus W11-5]
ATGGAACGCGTGCGTATCTTCAAGAACAATCAAAGTCGGGCCATTCGATTGCCCAAGGCACTGGACTTTTCACTGGATGT
CCAGGAGGTGGACATTATTCGCGTCGGCAATACGCGCATCATCGTACCGGCCGGACAGGGCTGGGCTACGTGGTTCGACC
GGGATGCCCGTGTCACGGATGATTTCATGGCCGAGCGGGAACAGCCGGATGATCAGGAACGGGATGCGCTGGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0B4XG05