Detailed information of TA system TA045023

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/upstrm_HI1419-dnstrm_HI1420
• Location: 2390316..2390971
• Replicon: chromosome
• Accession: NZ_CP004385
• Organism: Burkholderia thailandensis MSMB59

Toxin (Protein)

• Gene name: relE
• Uniprot ID: A0A0F6LEW6
• Locus tag: BTHA_RS11050
• Protein ID: WP_006027262.1
• Coordinates: 2390316..2390612 (+)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: Q63YL3
• Locus tag: BTHA_RS11055
• Protein ID: WP_004202809.1
• Coordinates: 2390615..2390971 (+)
• Length: 119 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BTHA_RS11015	2385656..2385922	+	267	WP_009909352.1	ogr/Delta-like zinc finger family protein	-
BTHA_RS11020	2385919..2386125	+	207	WP_004532731.1	ogr/Delta-like zinc finger family protein	-
BTHA_RS11025	2386215..2386868	+	654	WP_004532770.1	AAA family ATPase	-
BTHA_RS11030	2387102..2387224	+	123	WP_009897078.1	CopG family transcriptional regulator	-
BTHA_RS11035	2387327..2387860	+	534	WP_004532793.1	bacteriophage gp29 protein	-
BTHA_RS11040	2387857..2388594	+	738	WP_004532838.1	VRR-NUC domain-containing protein	-
BTHA_RS11045	2388609..2389724	+	1116	WP_038707992.1	DUF3396 domain-containing protein	-
BTHA_RS11050	2390316..2390612	+	297	WP_006027262.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BTHA_RS11055	2390615..2390971	+	357	WP_004202809.1	putative addiction module antidote protein	Antitoxin
BTHA_RS11060	2391015..2392070	-	1056	WP_009909353.1	phage portal protein	-
BTHA_RS11065	2392067..2393836	-	1770	WP_009909354.1	terminase ATPase subunit family protein	-
BTHA_RS11070	2393980..2394789	+	810	WP_004532870.1	GPO family capsid scaffolding protein	-
BTHA_RS11075	2394823..2395836	+	1014	WP_004532901.1	phage major capsid protein, P2 family	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2377441..2416756	39315

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (1-97)

Antitoxin

• Uncharacterized protein HI1420 (16-94)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 10844.65 Da
• Isoelectric Point: 10.1723

>T45023 WP_006027262.1 NZ_CP004385:2390316-2390612 [Burkholderia thailandensis MSMB59]
MFKVLTTPQFDKWLDGLRDPVGSAAINLRIERAKLGNLGQWRAVGDGVNEMKIDVGPGYRAYFVRRGKIIVVVLCGGDKS
TQKKDIKLAKQIAGELED

Nucleotide

• Length: 297 bp

>T45023 NZ_CP004385:2390316-2390612 [Burkholderia thailandensis MSMB59]
ATGTTCAAAGTTCTGACGACCCCCCAGTTTGACAAATGGCTTGACGGGCTTCGCGATCCGGTCGGTAGCGCGGCAATCAA
CCTGCGCATCGAGCGGGCGAAGCTTGGCAATCTCGGCCAGTGGCGCGCAGTCGGCGACGGCGTCAACGAAATGAAGATTG
ATGTGGGGCCGGGATATCGGGCCTATTTCGTGCGGCGCGGAAAAATTATCGTCGTGGTGTTGTGCGGCGGGGACAAGTCG
ACGCAGAAGAAGGACATCAAGCTAGCGAAGCAAATCGCTGGCGAACTGGAGGATTGA

Antitoxin

Protein

• Length: 119 a.a.
• Molecular weight: 12585.41 Da
• Isoelectric Point: 9.9470

>AT45023 WP_004202809.1 NZ_CP004385:2390615-2390971 [Burkholderia thailandensis MSMB59]
MKISELAEFDGSKYLKDEETIRHYLAQAFEDGNPRLIQAALGNVAKARGMTALARESGVKREALYRALSEGGNAEFATIM
KVVGALGLHLTVAPAEPAPVPAPATTRARSRVRTAAHA

Nucleotide

• Length: 357 bp

>AT45023 NZ_CP004385:2390615-2390971 [Burkholderia thailandensis MSMB59]
ATGAAAATCAGCGAACTGGCCGAGTTCGACGGCTCGAAGTACCTGAAGGACGAGGAAACGATTCGTCACTACCTGGCGCA
AGCGTTCGAGGATGGAAACCCGCGGCTGATTCAAGCCGCGCTCGGAAACGTCGCGAAAGCGCGCGGCATGACAGCGCTCG
CGCGCGAGTCCGGCGTGAAGCGTGAAGCGCTCTATCGCGCGCTGTCGGAAGGTGGGAACGCGGAATTCGCAACGATCATG
AAAGTTGTGGGCGCGTTGGGGCTGCACCTGACCGTTGCGCCGGCCGAACCTGCGCCGGTGCCCGCGCCGGCAACAACGCG
TGCACGCTCGCGCGTTCGCACGGCTGCGCACGCGTAA

Structures

Toxin

• AlphaFold DB: A0A0F6LEW6

Antitoxin

• AlphaFold DB: A0A3N4G1D3