Detailed information of TA system TA045017

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/upstrm_HI1419-dnstrm_HI1420
• Location: 229925..230580
• Replicon: chromosome
• Accession: NZ_CP004382
• Organism: Burkholderia thailandensis E254

Toxin (Protein)

• Gene name: relE
• Uniprot ID: A0A0F6LEW6
• Locus tag: BTN_RS18215
• Protein ID: WP_006027262.1
• Coordinates: 229925..230221 (+)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: Q63YL3
• Locus tag: BTN_RS18220
• Protein ID: WP_004202809.1
• Coordinates: 230224..230580 (+)
• Length: 119 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BTN_RS18180	225267..225533	+	267	WP_009971787.1	ogr/Delta-like zinc finger family protein	-
BTN_RS18185	225530..225736	+	207	WP_004532731.1	ogr/Delta-like zinc finger family protein	-
BTN_RS18190	225826..226479	+	654	WP_038778436.1	AAA family ATPase	-
BTN_RS18195	226713..226835	+	123	WP_009897078.1	CopG family transcriptional regulator	-
BTN_RS18200	226938..227471	+	534	WP_009897076.1	bacteriophage gp29 protein	-
BTN_RS18205	227468..228205	+	738	WP_043296749.1	VRR-NUC domain-containing protein	-
BTN_RS18210	228220..229334	+	1115	Protein_214	DUF3396 domain-containing protein	-
BTN_RS18215	229925..230221	+	297	WP_006027262.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BTN_RS18220	230224..230580	+	357	WP_004202809.1	putative addiction module antidote protein	Antitoxin
BTN_RS18225	230624..231679	-	1056	WP_023359646.1	phage portal protein	-
BTN_RS18230	231676..233445	-	1770	WP_043297030.1	terminase ATPase subunit family protein	-
BTN_RS18235	233589..234398	+	810	WP_015985028.1	GPO family capsid scaffolding protein	-
BTN_RS18240	234432..235445	+	1014	WP_038729266.1	phage major capsid protein, P2 family	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	216172..255965	39793

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (1-97)

Antitoxin

• Uncharacterized protein HI1420 (16-94)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 10844.65 Da
• Isoelectric Point: 10.1723

>T45017 WP_006027262.1 NZ_CP004382:229925-230221 [Burkholderia thailandensis E254]
MFKVLTTPQFDKWLDGLRDPVGSAAINLRIERAKLGNLGQWRAVGDGVNEMKIDVGPGYRAYFVRRGKIIVVVLCGGDKS
TQKKDIKLAKQIAGELED

Nucleotide

• Length: 297 bp

>T45017 NZ_CP004382:229925-230221 [Burkholderia thailandensis E254]
ATGTTCAAAGTTCTGACGACCCCCCAGTTTGACAAATGGCTTGACGGGCTTCGCGATCCGGTCGGTAGCGCGGCAATCAA
CCTGCGCATCGAGCGGGCGAAGCTTGGCAATCTCGGCCAGTGGCGCGCAGTCGGCGACGGCGTCAACGAAATGAAGATTG
ATGTAGGGCCGGGATATCGGGCCTACTTCGTGCGACGCGGAAAAATTATCGTCGTGGTGTTGTGCGGCGGGGACAAGTCG
ACGCAGAAGAAGGACATCAAGCTAGCGAAACAAATCGCTGGCGAACTGGAGGATTGA

Antitoxin

Protein

• Length: 119 a.a.
• Molecular weight: 12585.41 Da
• Isoelectric Point: 9.9470

>AT45017 WP_004202809.1 NZ_CP004382:230224-230580 [Burkholderia thailandensis E254]
MKISELAEFDGSKYLKDEETIRHYLAQAFEDGNPRLIQAALGNVAKARGMTALARESGVKREALYRALSEGGNAEFATIM
KVVGALGLHLTVAPAEPAPVPAPATTRARSRVRTAAHA

Nucleotide

• Length: 357 bp

>AT45017 NZ_CP004382:230224-230580 [Burkholderia thailandensis E254]
ATGAAAATCAGCGAACTGGCCGAGTTCGACGGCTCGAAGTACCTGAAGGACGAGGAAACGATTCGTCACTACCTGGCGCA
AGCGTTCGAGGATGGAAACCCGCGGCTGATTCAAGCCGCGCTCGGGAACGTCGCGAAAGCGCGCGGCATGACAGCGCTCG
CGCGCGAGTCCGGCGTGAAGCGTGAAGCGCTCTATCGCGCGCTGTCGGAAGGTGGGAACGCGGAATTCGCAACGATCATG
AAAGTTGTGGGCGCGTTGGGGCTGCACCTGACCGTTGCGCCGGCCGAACCTGCGCCGGTGCCCGCGCCGGCAACAACGCG
TGCACGCTCGCGCGTTCGCACGGCTGCGCACGCGTAA

Structures

Toxin

• AlphaFold DB: A0A0F6LEW6

Antitoxin

• AlphaFold DB: A0A3N4G1D3