Detailed information of TA system TA045014

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT_toxin-BrnA
• Location: 2398735..2399272
• Replicon: chromosome
• Accession: NZ_CP004380
• Organism: Burkholderia pseudomallei 1026b

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: -
• Locus tag: BBW_RS28265
• Protein ID: WP_009923450.1
• Coordinates: 2399003..2399272 (-)
• Length: 90 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: Q63NA3
• Locus tag: BBW_RS28260
• Protein ID: WP_004525420.1
• Coordinates: 2398735..2399019 (-)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BBW_RS28235	2394006..2395466	-	1461	WP_004538070.1	metallophosphoesterase	-
BBW_RS28240	2395732..2395848	+	117	Protein_1924	IS3 family transposase	-
BBW_RS37905	2396075..2396308	-	234	Protein_1925	transposase	-
BBW_RS28250	2396375..2397494	+	1120	Protein_1926	IS3 family transposase	-
BBW_RS37910	2397542..2397742	-	201	Protein_1927	IS110 family transposase	-
BBW_RS28255	2397705..2398088	-	384	Protein_1928	DDE-type integrase/transposase/recombinase	-
BBW_RS28260	2398735..2399019	-	285	WP_004525420.1	BrnA antitoxin family protein	Antitoxin
BBW_RS28265	2399003..2399272	-	270	WP_009923450.1	BrnT family toxin	Toxin
BBW_RS28270	2399407..2399865	+	459	Protein_1931	prohibitin family protein	-
BBW_RS28285	2401211..2401477	+	267	Protein_1933	transposase	-
BBW_RS28290	2401654..2403357	-	1704	WP_004553173.1	site-specific integrase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2390363..2428325	37962
	-	inside	IScluster/Tn	-	-	2390363..2401477	11114

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-81)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (27-88)

Sequences

Toxin

Protein

• Length: 90 a.a.
• Molecular weight: 10156.52 Da
• Isoelectric Point: 7.1633

>T45014 WP_009923450.1 NZ_CP004380:c2399272-2399003 [Burkholderia pseudomallei 1026b]
MDITFDPTKNKTNIAKHGVSLALAAQLDWSDVLSYVDDRRDYSEVREVGFGVIGDRLYCVVFTQRGDSMHIISMRKANKR
EVKSYVEQA

Nucleotide

• Length: 270 bp

>T45014 NZ_CP004380:c2399272-2399003 [Burkholderia pseudomallei 1026b]
ATGGACATCACCTTTGACCCGACCAAGAACAAAACGAACATTGCCAAGCATGGAGTGTCGTTGGCGCTTGCAGCGCAACT
CGACTGGTCGGACGTGTTGTCCTACGTGGACGACCGACGCGACTACAGTGAAGTGCGCGAAGTCGGGTTCGGTGTGATCG
GCGATCGCCTCTATTGCGTGGTGTTCACGCAGCGCGGCGACTCGATGCACATCATCAGCATGCGTAAGGCGAACAAGCGG
GAGGTCAAGAGCTATGTCGAGCAAGCGTAA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10697.10 Da
• Isoelectric Point: 5.8930

>AT45014 WP_004525420.1 NZ_CP004380:c2399019-2398735 [Burkholderia pseudomallei 1026b]
MSSKRKIVMPTDEEDAAINRGIAADPDTFEVPAEDFAKMTRRGKRGRPPLEAPKVQLTVRYDVDIVDAFKATGEGWQTRM
NDALREWLREHQPA

Nucleotide

• Length: 285 bp

>AT45014 NZ_CP004380:c2399019-2398735 [Burkholderia pseudomallei 1026b]
ATGTCGAGCAAGCGTAAGATCGTCATGCCGACGGACGAGGAAGACGCGGCAATCAACCGCGGCATCGCGGCCGATCCCGA
CACGTTCGAAGTGCCGGCGGAAGACTTCGCGAAGATGACGCGGCGAGGCAAGCGTGGTCGTCCGCCGCTCGAAGCGCCCA
AGGTGCAATTGACGGTGCGCTATGACGTCGACATTGTCGACGCCTTCAAAGCGACGGGCGAAGGTTGGCAGACGCGTATG
AATGATGCGTTGCGCGAGTGGCTGAGGGAACATCAGCCTGCGTAG

Structures

Antitoxin

• AlphaFold DB: A0A0F6L9S8