Detailed information of TA system TA045010

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT_toxin-BrnA
• Location: 2915899..2916388
• Replicon: chromosome
• Accession: NZ_CP004378
• Organism: Burkholderia pseudomallei NAU35A-3

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: -
• Locus tag: BBU_RS29525
• Protein ID: WP_038734759.1
• Coordinates: 2916167..2916388 (-)
• Length: 74 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: Q2T6G1
• Locus tag: BBU_RS29520
• Protein ID: WP_011401115.1
• Coordinates: 2915899..2916183 (-)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BBU_RS29490	2912049..2912234	-	186	WP_038725166.1	hypothetical protein	-
BBU_RS29495	2912231..2913943	-	1713	WP_038725169.1	terminase large subunit	-
BBU_RS29500	2913953..2914438	-	486	WP_076861667.1	phage terminase small subunit P27 family	-
BBU_RS29505	2914586..2914942	-	357	WP_004548856.1	HNH endonuclease	-
BBU_RS38265	2915001..2915258	+	258	WP_004549735.1	type II toxin-antitoxin system RelE/ParE family toxin	-
BBU_RS29510	2915255..2915641	+	387	WP_004552941.1	helix-turn-helix domain-containing protein	-
BBU_RS40090	2915738..2915902	-	165	WP_004549060.1	hypothetical protein	-
BBU_RS29520	2915899..2916183	-	285	WP_011401115.1	BrnA antitoxin family protein	Antitoxin
BBU_RS29525	2916167..2916388	-	222	WP_038734759.1	BrnT family toxin	Toxin
BBU_RS29530	2916487..2916930	-	444	WP_038796648.1	hypothetical protein	-
BBU_RS29535	2916927..2917187	-	261	WP_038725177.1	hypothetical protein	-
BBU_RS29540	2917498..2917923	-	426	WP_004526654.1	DUF1364 family protein	-
BBU_RS29545	2917938..2918285	-	348	WP_038738298.1	DUF2591 family protein	-
BBU_RS29550	2918474..2918959	-	486	Protein_2378	DUF1064 domain-containing protein	-
BBU_RS29555	2918956..2919948	-	993	WP_038757830.1	hypothetical protein	-
BBU_RS29560	2920102..2920362	-	261	WP_004526650.1	helix-turn-helix domain-containing protein	-
BBU_RS29565	2920359..2921180	-	822	WP_038757827.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	2883656..2941505	57849

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (14-65)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (27-88)

Sequences

Toxin

Protein

• Length: 74 a.a.
• Molecular weight: 8606.71 Da
• Isoelectric Point: 8.4920

>T45010 WP_038734759.1 NZ_CP004378:c2916388-2916167 [Burkholderia pseudomallei NAU35A-3]
MECRWRLQRNSTGQDVLSYVDDRRDYSEVREVGFGVIGDRLYCVVFTQRGDSMHIISMRKANKREAKSYVEQA

Nucleotide

• Length: 222 bp

>T45010 NZ_CP004378:c2916388-2916167 [Burkholderia pseudomallei NAU35A-3]
ATGGAGTGTCGTTGGCGCTTGCAGCGCAACTCGACTGGTCAGGACGTGTTGTCCTACGTGGACGACCGACGCGACTACAG
TGAAGTGCGCGAAGTCGGGTTCGGTGTGATCGGCGATCGCCTCTATTGCGTGGTGTTCACGCAGCGCGGCGACTCGATGC
ACATCATCAGCATGCGTAAGGCGAACAAGCGGGAGGCCAAGAGCTATGTCGAGCAAGCGTAA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10669.09 Da
• Isoelectric Point: 5.8930

>AT45010 WP_011401115.1 NZ_CP004378:c2916183-2915899 [Burkholderia pseudomallei NAU35A-3]
MSSKRKIVMPTDEEDAAINRGIAADPDTFEVPAEDFAKMTRRGKRGRPPLEAPKVQLTVRYDVDIVDAFKATGEGWQTRM
NDALREWLKEHQPA

Nucleotide

• Length: 285 bp

>AT45010 NZ_CP004378:c2916183-2915899 [Burkholderia pseudomallei NAU35A-3]
ATGTCGAGCAAGCGTAAGATCGTCATGCCGACGGACGAGGAAGACGCGGCAATCAACCGCGGCATCGCGGCCGATCCCGA
CACGTTCGAAGTGCCGGCGGAAGACTTCGCGAAGATGACGCGGCGAGGCAAGCGTGGTCGTCCGCCGCTCGAAGCGCCCA
AGGTGCAATTGACCGTGCGCTATGACGTCGACATTGTCGACGCCTTCAAAGCGACGGGCGAAGGTTGGCAGACGCGCATG
AATGATGCGTTGCGCGAGTGGCTGAAGGAGCACCAGCCTGCGTGA

Structures

Antitoxin

• AlphaFold DB: Q2T6G1