Detailed information of TA system TA045003

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT_toxin-BrnA
• Location: 2393972..2394509
• Replicon: chromosome
• Accession: NZ_CP004369
• Organism: Burkholderia pseudomallei MSHR520

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: -
• Locus tag: BBX_RS28160
• Protein ID: WP_009923450.1
• Coordinates: 2393972..2394241 (+)
• Length: 90 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: Q63NA3
• Locus tag: BBX_RS28165
• Protein ID: WP_004525420.1
• Coordinates: 2394225..2394509 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BBX_RS42330	2389949..2390377	-	429	Protein_1954	DDE-type integrase/transposase/recombinase	-
BBX_RS42335	2390375..2391148	-	774	Protein_1955	prohibitin family protein	-
BBX_RS28120	2391145..2391294	-	150	WP_004542626.1	bacteriophage protein Gp44	-
BBX_RS28125	2391550..2392131	+	582	WP_004542548.1	hypothetical protein	-
BBX_RS41970	2392362..2392580	-	219	WP_004552392.1	hypothetical protein	-
BBX_RS28135	2392628..2392849	-	222	WP_004525418.1	hypothetical protein	-
BBX_RS28150	2393126..2393401	-	276	WP_004552388.1	bacteriophage protein Gp49	-
BBX_RS28160	2393972..2394241	+	270	WP_009923450.1	BrnT family toxin	Toxin
BBX_RS28165	2394225..2394509	+	285	WP_004525420.1	BrnA antitoxin family protein	Antitoxin
BBX_RS28175	2395156..2395539	+	384	Protein_1963	DDE-type integrase/transposase/recombinase	-
BBX_RS38815	2395502..2395930	+	429	Protein_1964	IS110 family transposase	-
BBX_RS28180	2396157..2396273	-	117	Protein_1965	IS3 family transposase	-
BBX_RS28185	2396520..2397980	+	1461	WP_020850157.1	metallophosphoesterase	-
BBX_RS28190	2398139..2399401	+	1263	WP_020850375.1	cytochrome c family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2387638..2401623	13985

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-81)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (27-88)

Sequences

Toxin

Protein

• Length: 90 a.a.
• Molecular weight: 10156.52 Da
• Isoelectric Point: 7.1633

>T45003 WP_009923450.1 NZ_CP004369:2393972-2394241 [Burkholderia pseudomallei MSHR520]
MDITFDPTKNKTNIAKHGVSLALAAQLDWSDVLSYVDDRRDYSEVREVGFGVIGDRLYCVVFTQRGDSMHIISMRKANKR
EVKSYVEQA

Nucleotide

• Length: 270 bp

>T45003 NZ_CP004369:2393972-2394241 [Burkholderia pseudomallei MSHR520]
ATGGACATCACCTTTGACCCGACCAAGAACAAAACGAACATTGCCAAGCATGGAGTGTCGTTGGCGCTTGCAGCGCAACT
CGACTGGTCGGACGTGTTGTCCTACGTGGACGACCGACGCGACTACAGTGAAGTGCGCGAAGTCGGGTTCGGTGTGATCG
GCGATCGCCTCTATTGCGTGGTGTTCACGCAGCGCGGCGACTCGATGCACATCATCAGCATGCGTAAGGCGAACAAGCGG
GAGGTCAAGAGCTATGTCGAGCAAGCGTAA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10697.10 Da
• Isoelectric Point: 5.8930

>AT45003 WP_004525420.1 NZ_CP004369:2394225-2394509 [Burkholderia pseudomallei MSHR520]
MSSKRKIVMPTDEEDAAINRGIAADPDTFEVPAEDFAKMTRRGKRGRPPLEAPKVQLTVRYDVDIVDAFKATGEGWQTRM
NDALREWLREHQPA

Nucleotide

• Length: 285 bp

>AT45003 NZ_CP004369:2394225-2394509 [Burkholderia pseudomallei MSHR520]
ATGTCGAGCAAGCGTAAGATCGTCATGCCGACGGACGAGGAAGACGCGGCAATCAACCGCGGCATCGCGGCCGATCCCGA
CACGTTCGAAGTGCCGGCGGAAGACTTCGCGAAGATGACGCGGCGAGGCAAGCGTGGTCGTCCGCCGCTCGAAGCGCCCA
AGGTGCAATTGACGGTGCGCTATGACGTCGACATTGTCGACGCCTTCAAAGCGACGGGCGAAGGTTGGCAGACGCGCATG
AATGATGCGTTACGCGAGTGGCTGAGGGAACATCAGCCTGCGTAG

Structures

Antitoxin

• AlphaFold DB: A0A0F6L9S8