Detailed information of TA system TA045002

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 701581..703322
• Replicon: chromosome
• Accession: NZ_CP004369
• Organism: Burkholderia pseudomallei MSHR520

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: A0A0F6L1Z5
• Locus tag: BBX_RS21075
• Protein ID: WP_004542147.1
• Coordinates: 701970..703322 (+)
• Length: 451 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: BBX_RS21070
• Protein ID: WP_004542113.1
• Coordinates: 701581..701973 (+)
• Length: 131 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BBX_RS21060	698118..700514	-	2397	WP_043944143.1	cellulose biosynthesis cyclic di-GMP-binding regulatory protein BcsB	-
BBX_RS41790	700980..701243	+	264	WP_004542097.1	hypothetical protein	-
BBX_RS21070	701581..701973	+	393	WP_004542113.1	helix-turn-helix domain-containing protein	Antitoxin
BBX_RS21075	701970..703322	+	1353	WP_004542147.1	type II toxin-antitoxin system HipA family toxin	Toxin
BBX_RS21080	703479..703931	-	453	WP_004198842.1	hypothetical protein	-
BBX_RS21090	704334..705248	-	915	WP_004198844.1	LysR family transcriptional regulator	-
BBX_RS21095	705415..706368	+	954	WP_004542121.1	agmatinase	-
BBX_RS21100	706645..706986	+	342	WP_004525272.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (158-406)

Antitoxin

• Cro/C1-type helix-turn-helix domain (22-71)

Sequences

Toxin

Protein

• Length: 451 a.a.
• Molecular weight: 49818.85 Da
• Isoelectric Point: 9.8300

>T45002 WP_004542147.1 NZ_CP004369:701970-703322 [Burkholderia pseudomallei MSHR520]
VSARRARATRLHLWMNGLPVGYWEHARDGERLVYFDEWIGDPQGRPLSLSLPFTPGNQPYRGRLVSDYFDNLLPDSEPIR
RRIAMRYRTGGTSAFALLATLGRDCVGALQMLPPDEAPDDIERIRGHVLADADIARLLREVTSAPQAGRHAPLDDLRLSI
AGAQEKTALLRHRGRWLLPEGSTPTTHILKLPLGLVGNRRADMRTSVENEWLCARIVAAYGLPVARCDIAQFDDQKALVV
ERFDRRPSRDARWLLRLPQEDMCQATGTSALDKYQADGGPGIETIMEVLAGSEHARDDRRAFFAAQIVFWLLAATDGHAK
NFSIAHLPGNRYRSTPLYDVLSAHPVIGRGANQLPAQRARLAMGVRGKHIHYPLHQIRRRHWIAQGQRVGFAPADVDALI
DTLTARTAGVVDAVSARLPRDFPRDVADAIFSGMLGLSARLAGDAAARAP

Nucleotide

• Length: 1353 bp

>T45002 NZ_CP004369:701970-703322 [Burkholderia pseudomallei MSHR520]
GTGAGCGCCCGCCGCGCACGCGCGACGCGCCTGCACCTGTGGATGAACGGCCTGCCCGTCGGCTACTGGGAGCACGCACG
CGACGGCGAGCGCCTTGTCTACTTCGACGAATGGATCGGCGATCCGCAAGGCCGGCCGCTGTCGCTGTCGCTGCCGTTCA
CGCCGGGCAACCAGCCGTATCGCGGTCGGCTCGTCAGCGATTATTTCGACAACCTGCTGCCCGACAGCGAGCCGATCCGC
CGGCGAATCGCGATGCGCTACCGCACGGGCGGCACGTCCGCGTTCGCGCTGCTCGCGACGCTCGGCCGCGATTGCGTCGG
CGCGCTGCAGATGCTGCCGCCCGACGAAGCGCCGGACGACATCGAACGCATCCGCGGCCACGTGCTCGCCGACGCGGACA
TCGCGCGCCTGCTGCGCGAAGTCACGTCCGCGCCGCAGGCCGGCCGGCACGCGCCGCTCGACGATCTGCGCCTGTCGATC
GCCGGCGCGCAGGAGAAGACCGCGCTGCTGCGCCATCGCGGCCGCTGGCTGCTGCCCGAAGGGAGCACGCCGACCACGCA
CATCCTGAAGCTGCCGCTCGGGCTCGTCGGCAACCGGCGCGCCGACATGCGCACGTCGGTCGAGAACGAATGGCTGTGCG
CGCGGATCGTCGCCGCGTACGGGTTGCCCGTCGCGCGCTGCGACATCGCGCAGTTCGACGATCAGAAAGCGCTCGTCGTC
GAGCGCTTCGACCGCCGGCCGTCGCGCGACGCGCGCTGGCTCCTGCGGCTGCCGCAGGAAGACATGTGCCAGGCAACCGG
CACGTCCGCGCTCGACAAATATCAGGCCGACGGCGGCCCCGGCATCGAGACGATCATGGAAGTGCTCGCCGGCTCCGAAC
ACGCGCGGGACGACCGCCGCGCGTTCTTCGCGGCGCAGATCGTGTTCTGGCTGCTCGCCGCGACCGACGGCCACGCGAAG
AACTTCAGCATCGCGCACCTGCCCGGCAACCGCTACCGTTCGACGCCGCTTTACGACGTGCTGTCCGCGCATCCGGTCAT
CGGCCGGGGCGCGAACCAGTTGCCCGCGCAGCGCGCGCGGCTCGCGATGGGCGTGCGCGGCAAGCACATCCACTATCCGC
TGCACCAGATCCGGCGGCGGCACTGGATCGCGCAGGGCCAGCGCGTCGGCTTCGCGCCCGCCGACGTCGACGCGCTGATC
GACACGCTGACCGCGCGCACCGCGGGCGTCGTCGACGCGGTGTCGGCGCGGCTGCCGCGCGATTTTCCGCGCGACGTCGC
CGATGCGATCTTCAGCGGAATGCTCGGCCTGAGCGCAAGGCTCGCCGGCGACGCGGCCGCGCGCGCGCCATGA

Antitoxin

Protein

• Length: 131 a.a.
• Molecular weight: 13912.14 Da
• Isoelectric Point: 11.5433

>AT45002 WP_004542113.1 NZ_CP004369:701581-701973 [Burkholderia pseudomallei MSHR520]
MAILIEHEIKTLGQLRPILRGFRKSAGLTQAMLASRLGVTQQTYAQFEANPASASVERLFKVLRALDIELTLTLTQVYAA
PAGNDKGEVAKTAAGARRAVPPASAPAPSAAGRAPRPARKRAAPKKREDW

Nucleotide

• Length: 393 bp

>AT45002 NZ_CP004369:701581-701973 [Burkholderia pseudomallei MSHR520]
ATGGCCATCCTCATCGAGCACGAGATCAAGACACTCGGCCAGCTGCGGCCGATTCTGCGCGGCTTCCGCAAATCGGCCGG
ATTGACGCAGGCGATGCTCGCGAGCCGCCTCGGCGTCACGCAGCAGACCTACGCGCAGTTCGAGGCGAACCCGGCGTCGG
CGAGCGTCGAGCGGCTGTTCAAGGTGCTGCGCGCGCTCGACATCGAACTCACGCTCACGCTCACGCAGGTCTACGCCGCG
CCCGCGGGCAACGACAAGGGCGAGGTTGCGAAGACGGCCGCAGGCGCGCGACGTGCCGTGCCGCCCGCGTCCGCGCCCGC
TCCGAGCGCCGCCGGGCGCGCGCCCCGCCCCGCCCGCAAGCGCGCCGCCCCGAAAAAGCGGGAGGACTGGTGA

Structures

Toxin

• AlphaFold DB: A0A0F6L1Z5