Detailed information of TA system TA045001

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PP_4151-4152/excise(antitoxin)
• Location: 1832240..1833281
• Replicon: chromosome
• Accession: NZ_CP004368
• Organism: Burkholderia pseudomallei MSHR520

Toxin (Protein)

• Gene name: PP_4152
• Uniprot ID: -
• Locus tag: BBX_RS08600
• Protein ID: WP_004539802.1
• Coordinates: 1832706..1833281 (+)
• Length: 192 a.a.

Antitoxin (Protein)

• Gene name: PP_4151
• Uniprot ID: A0A0E1SLI7
• Locus tag: BBX_RS08595
• Protein ID: WP_004540053.1
• Coordinates: 1832240..1832701 (+)
• Length: 154 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BBX_RS08570	1827846..1828514	-	669	WP_012730038.1	restriction endonuclease fold toxin 5 domain-containing protein	-
BBX_RS08580	1828799..1829305	-	507	WP_004539924.1	DUF4123 domain-containing protein	-
BBX_RS08585	1829302..1829727	-	426	WP_004539640.1	PAAR domain-containing protein	-
BBX_RS40205	1830125..1830505	-	381	WP_020850431.1	hypothetical protein	-
BBX_RS34010	1830965..1831192	-	228	WP_004540114.1	hypothetical protein	-
BBX_RS40210	1831189..1831587	-	399	WP_133604798.1	hypothetical protein	-
BBX_RS08595	1832240..1832701	+	462	WP_004540053.1	excisionase family DNA-binding protein	Antitoxin
BBX_RS08600	1832706..1833281	+	576	WP_004539802.1	PIN domain-containing protein	Toxin
BBX_RS34020	1833467..1835683	+	2217	WP_004540097.1	ATP-binding protein	-
BBX_RS08605	1836932..1837111	+	180	WP_004191077.1	hypothetical protein	-
BBX_RS08610	1837295..1837567	+	273	WP_004527347.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-114)

Antitoxin

• Helix-turn-helix domain, group 17 (83-129)

Sequences

Toxin

Protein

• Length: 192 a.a.
• Molecular weight: 22056.35 Da
• Isoelectric Point: 6.4079

>T45001 WP_004539802.1 NZ_CP004368:1832706-1833281 [Burkholderia pseudomallei MSHR520]
MSSNFTVVYDACVLYPAPLRDLLMHLAVTDLYRARWTDMIHDEWTRNLLAKRTDLKPEAIQRTRELMDMHVRDAKVENFE
HLIAAIELPDPDDRHVVAAAMHAGAELIVTMNIKDFPRERVGKYNVSAQHPDDFIVDLWDLNKHKVMEAVARHRKSMKNP
PKSVEDYLDTMANQGLVQTVDALRPYAPWAI

Nucleotide

• Length: 576 bp

>T45001 NZ_CP004368:1832706-1833281 [Burkholderia pseudomallei MSHR520]
ATGTCGTCGAACTTTACCGTTGTCTACGATGCGTGCGTCCTATATCCGGCTCCGTTGCGCGACTTGTTGATGCATCTCGC
GGTGACGGATCTGTACCGCGCGCGTTGGACCGATATGATTCATGACGAGTGGACAAGGAATTTGCTCGCGAAGCGAACCG
ACCTGAAACCCGAGGCGATCCAGCGCACGCGGGAACTGATGGACATGCATGTTCGCGACGCGAAAGTGGAAAACTTCGAG
CATCTGATTGCGGCGATCGAGTTGCCCGATCCTGATGATCGTCATGTCGTCGCGGCAGCCATGCATGCTGGGGCGGAACT
CATCGTCACGATGAACATCAAGGATTTCCCGAGGGAGCGGGTCGGAAAGTACAACGTGTCGGCTCAACATCCCGACGATT
TCATCGTCGACTTGTGGGACTTGAACAAGCACAAGGTGATGGAGGCGGTCGCGCGACATCGCAAGTCGATGAAGAACCCG
CCGAAGTCAGTGGAAGACTATCTCGACACGATGGCCAATCAGGGACTCGTGCAGACGGTCGATGCGCTGCGGCCGTATGC
GCCTTGGGCTATCTGA

Antitoxin

Protein

• Length: 154 a.a.
• Molecular weight: 17155.41 Da
• Isoelectric Point: 5.5474

>AT45001 WP_004540053.1 NZ_CP004368:1832240-1832701 [Burkholderia pseudomallei MSHR520]
MNTTVTPAALPSEHEAELARESRRNLVTVVNSHAETQQFDFKDEKGETHTVTLPTSALRLLDEILHQIGNGNAVSIIPIH
AELTTQQAADLLNVSRPYLVQLLEQGEIPFRKVNTHRRVMYLDLIQYKNKIDANRLDALAELTAQAQELGMGY

Nucleotide

• Length: 462 bp

>AT45001 NZ_CP004368:1832240-1832701 [Burkholderia pseudomallei MSHR520]
ATGAACACCACTGTCACTCCTGCGGCCCTCCCGTCCGAACACGAGGCCGAGCTCGCGCGCGAGTCGCGGCGGAATTTGGT
CACGGTCGTGAACAGCCACGCCGAGACTCAGCAGTTTGATTTCAAGGACGAAAAGGGGGAGACGCATACAGTCACGCTTC
CGACCTCGGCGTTAAGGCTGCTAGACGAGATCCTCCATCAGATCGGAAATGGCAACGCGGTGTCGATCATTCCAATCCAT
GCCGAATTGACGACCCAGCAAGCCGCCGACCTTCTGAATGTCTCGCGTCCGTACCTGGTTCAACTTCTTGAGCAGGGAGA
GATCCCATTCCGGAAGGTAAATACGCATCGACGGGTGATGTATCTGGATCTGATTCAGTACAAAAACAAGATCGATGCGA
ACCGTCTCGACGCGCTGGCTGAACTGACGGCTCAGGCGCAGGAACTTGGAATGGGATATTGA

Structures

Antitoxin

• AlphaFold DB: A0A0E1SLI7