Detailed information of TA system TA045000

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relB-parE/ParE-RHH
• Location: 881132..881688
• Replicon: chromosome
• Accession: NZ_CP004368
• Organism: Burkholderia pseudomallei MSHR520

Toxin (Protein)

• Gene name: parE
• Uniprot ID: -
• Locus tag: BBX_RS04155
• Protein ID: WP_023594135.1
• Coordinates: 881398..881688 (+)
• Length: 97 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: M4UBZ0
• Locus tag: BBX_RS04150
• Protein ID: WP_004541313.1
• Coordinates: 881132..881410 (+)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BBX_RS33230	877033..877929	-	897	WP_020850700.1	hypothetical protein	-
BBX_RS33235	878007..878264	-	258	WP_004541318.1	hypothetical protein	-
BBX_RS04140	878755..879024	+	270	WP_020850841.1	ribbon-helix-helix protein, CopG family	-
BBX_RS04145	879027..881051	+	2025	WP_196043030.1	relaxase/mobilization nuclease domain-containing protein	-
BBX_RS04150	881132..881410	+	279	WP_004541313.1	CopG family ribbon-helix-helix protein	Antitoxin
BBX_RS04155	881398..881688	+	291	WP_023594135.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BBX_RS04160	881685..882005	+	321	WP_197540451.1	hypothetical protein	-
BBX_RS40100	882201..882449	+	249	WP_004541364.1	hypothetical protein	-
BBX_RS42115	882554..883213	+	660	WP_020850627.1	hypothetical protein	-
BBX_RS04175	883255..885456	-	2202	WP_004541389.1	AAA family ATPase	-
BBX_RS04180	885683..886204	-	522	WP_004541357.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	873554..890702	17148

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-94)

Antitoxin

• Ribbon-helix-helix protein, CopG (5-42)

Sequences

Toxin

Protein

• Length: 97 a.a.
• Molecular weight: 10892.45 Da
• Isoelectric Point: 9.5554

>T45000 WP_023594135.1 NZ_CP004368:881398-881688 [Burkholderia pseudomallei MSHR520]
MPQVIFAPAAIGDMQRLREFLRPKNPDAARRAGEAIRRGVQALGAHPRMGRLIEDLPEQYREWLIDFGDSGYVARYRVDG
DTVTILAVRHQKEAGY

Nucleotide

• Length: 291 bp

>T45000 NZ_CP004368:881398-881688 [Burkholderia pseudomallei MSHR520]
ATGCCACAAGTAATCTTTGCGCCGGCGGCGATCGGGGACATGCAACGGCTCCGCGAATTCCTGAGGCCGAAGAATCCCGA
TGCCGCGAGGCGGGCCGGCGAAGCGATTAGGCGCGGCGTCCAGGCTCTCGGTGCCCACCCTCGAATGGGGCGTCTCATAG
AAGATCTGCCTGAACAGTATCGGGAGTGGCTTATCGATTTTGGGGATAGTGGCTATGTGGCTCGGTACCGCGTCGATGGC
GATACCGTCACGATTTTGGCCGTGCGACACCAGAAGGAAGCAGGGTACTGA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10810.11 Da
• Isoelectric Point: 4.9736

>AT45000 WP_004541313.1 NZ_CP004368:881132-881410 [Burkholderia pseudomallei MSHR520]
MVTATSIKLDDELKGRVQHLAEARRRTPHWIMREAIEQYVEREEKREALNRDTLKAWDEFQATGLHVTAEEVDKWLASWG
TDDELPPPECHK

Nucleotide

• Length: 279 bp

>AT45000 NZ_CP004368:881132-881410 [Burkholderia pseudomallei MSHR520]
ATGGTTACGGCAACATCCATCAAGCTTGATGATGAATTGAAAGGGCGGGTTCAGCACTTGGCCGAGGCGCGCCGGCGTAC
CCCGCACTGGATCATGCGCGAAGCCATTGAGCAGTACGTCGAGCGTGAGGAAAAGCGCGAGGCGCTGAACCGGGACACGC
TCAAGGCATGGGATGAATTTCAGGCGACTGGCCTGCACGTGACCGCTGAGGAAGTGGACAAGTGGCTCGCGAGCTGGGGA
ACTGACGACGAACTGCCTCCGCCAGAATGCCACAAGTAA

Structures

Antitoxin

• AlphaFold DB: M4UBZ0