Detailed information of TA system TA044997

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 69666..70301
• Replicon: plasmid pGX5
• Accession: NZ_CP004365
• Organism: Komagataeibacter xylinus E25

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: H845_RS16680
• Protein ID: WP_010517707.1
• Coordinates: 69666..70076 (-)
• Length: 137 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A0N1FCF7
• Locus tag: H845_RS19475
• Protein ID: WP_025440224.1
• Coordinates: 70077..70301 (-)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H845_RS19110	64851..65186	+	336	Protein_72	helix-turn-helix domain-containing protein	-
H845_RS16660	66488..67233	+	746	Protein_74	IS630 family transposase	-
H845_RS16665	67246..67551	-	306	WP_025440035.1	CcdB family protein	-
H845_RS16670	67552..67752	-	201	WP_010512663.1	type II toxin-antitoxin system CcdA family antitoxin	-
H845_RS16675	67939..69309	-	1371	WP_025440036.1	hypothetical protein	-
H845_RS16680	69666..70076	-	411	WP_010517707.1	type II toxin-antitoxin system VapC family toxin	Toxin
H845_RS19475	70077..70301	-	225	WP_025440224.1	hypothetical protein	Antitoxin
H845_RS19480	70410..70676	-	267	Protein_80	IS3 family transposase	-
H845_RS19120	70702..70908	-	207	Protein_81	transposase domain-containing protein	-
H845_RS16695	71024..72459	-	1436	Protein_82	Si-specific NAD(P)(+) transhydrogenase	-
H845_RS16700	72544..72862	+	319	Protein_83	WHG domain-containing protein	-
H845_RS19125	72993..74050	+	1058	WP_085948209.1	IS630 family transposase	-
H845_RS16715	74126..74896	+	771	WP_025440037.1	alpha/beta hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..336138	336138
	-	flank	IS/Tn	-	-	73499..74050	551

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-124)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 137 a.a.
• Molecular weight: 15462.66 Da
• Isoelectric Point: 6.4800

>T44997 WP_010517707.1 NZ_CP004365:c70076-69666 [Komagataeibacter xylinus E25]
MLRYLLDTNLCIRVLRDRPQGLRPRFNSCAEELCISDVVLYELLYGAERSSDPVHTRREVEHFAARLAVLPFDSEAAAHT
ADIRAALERNGRIIGPYDLMIAGHARSRGLIVVTGNLREFQRVEGLRTEDWLTDTV

Nucleotide

• Length: 411 bp

>T44997 NZ_CP004365:c70076-69666 [Komagataeibacter xylinus E25]
ATGCTGCGTTACCTGCTGGATACGAACCTCTGTATCCGGGTGTTGCGGGATCGACCGCAGGGGCTGCGTCCGAGGTTCAA
CAGCTGTGCGGAGGAACTCTGCATCTCGGACGTGGTGCTGTATGAGCTGCTTTATGGCGCGGAACGCTCGTCTGATCCCG
TGCACACAAGGCGTGAGGTCGAGCACTTCGCGGCAAGGCTGGCGGTCCTGCCATTCGACAGTGAAGCGGCCGCCCATACA
GCGGACATTCGGGCTGCTCTTGAACGGAATGGCCGTATCATAGGTCCGTATGATCTCATGATTGCCGGGCATGCCCGGAG
CAGGGGGCTGATTGTGGTGACCGGCAATCTGCGGGAATTCCAGCGGGTGGAAGGTCTTCGAACCGAGGACTGGCTGACTG
ACACGGTCTGA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8297.56 Da
• Isoelectric Point: 5.0001

>AT44997 WP_025440224.1 NZ_CP004365:c70301-70077 [Komagataeibacter xylinus E25]
MLTRTTLFLSNRSQAVRLPKMVAFGEQVREVVIVSEGPRRIIAPVDVAWDDFFAAPGVDLGERNQPAMQEREAL

Nucleotide

• Length: 225 bp

>AT44997 NZ_CP004365:c70301-70077 [Komagataeibacter xylinus E25]
ATGCTGACCCGTACAACACTCTTTCTATCCAATCGCTCGCAGGCGGTGCGTCTGCCGAAGATGGTCGCCTTCGGGGAACA
GGTGCGTGAGGTGGTCATCGTGTCCGAAGGTCCACGGCGGATCATCGCTCCGGTCGATGTGGCGTGGGATGATTTCTTTG
CGGCTCCCGGGGTGGATCTGGGGGAGCGGAACCAGCCAGCCATGCAGGAACGCGAGGCGCTCTGA

Structures

Antitoxin

• AlphaFold DB: A0A0N1FCF7