Detailed information of TA system TA044996

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 39117..39723
• Replicon: plasmid pGX4
• Accession: NZ_CP004364
• Organism: Komagataeibacter xylinus E25

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: H845_RS18935
• Protein ID: WP_010518064.1
• Coordinates: 39544..39723 (-)
• Length: 60 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: W5YE06
• Locus tag: H845_RS16145
• Protein ID: WP_010518062.1
• Coordinates: 39117..39533 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H845_RS19945	34904..35047	+	144	WP_157699282.1	hypothetical protein	-
H845_RS16100	35091..35477	-	387	WP_025439964.1	type II toxin-antitoxin system VapC family toxin	-
H845_RS16105	35474..35719	-	246	WP_010518373.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
H845_RS18925	35740..36000	-	261	WP_034931139.1	type II toxin-antitoxin system HicA family toxin	-
H845_RS20185	36073..36225	+	153	WP_165165566.1	hypothetical protein	-
H845_RS16110	36255..36578	-	324	WP_025439965.1	type II toxin-antitoxin system PemK/MazF family toxin	-
H845_RS16115	36575..36793	-	219	WP_025439966.1	antitoxin MazE family protein	-
H845_RS18930	37029..37355	-	327	WP_117125944.1	DUF2442 domain-containing protein	-
H845_RS16120	37448..37963	-	516	WP_010518057.1	hypothetical protein	-
H845_RS20190	37869..38186	-	318	WP_167331481.1	hypothetical protein	-
H845_RS16130	38249..38434	-	186	WP_010518059.1	PilT protein	-
H845_RS16135	38431..38688	-	258	WP_019087450.1	plasmid stabilization protein	-
H845_RS16140	38786..39040	-	255	WP_081749535.1	hypothetical protein	-
H845_RS16145	39117..39533	-	417	WP_010518062.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
H845_RS18935	39544..39723	-	180	WP_010518064.1	type II toxin-antitoxin system HicA family toxin	Toxin
H845_RS18940	40500..41615	-	1116	WP_167331479.1	IS110 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..87176	87176

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (4-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-125)

Sequences

Toxin

Protein

• Length: 60 a.a.
• Molecular weight: 6509.68 Da
• Isoelectric Point: 11.4111

>T44996 WP_010518064.1 NZ_CP004364:c39723-39544 [Komagataeibacter xylinus E25]
MKSADLIRELEKTGWKLDRVRGSHNVFKHPSRPGIVVVPHPKKDLGTGLVAAIRKQAGL

Nucleotide

• Length: 180 bp

>T44996 NZ_CP004364:c39723-39544 [Komagataeibacter xylinus E25]
ATGAAGAGTGCCGATCTGATCCGGGAGCTGGAAAAAACAGGCTGGAAGCTGGATCGCGTGCGCGGTTCACACAACGTCTT
CAAACATCCGTCGCGACCAGGGATCGTGGTCGTCCCTCATCCCAAAAAGGATCTGGGCACCGGTCTGGTTGCCGCCATCC
GCAAACAGGCGGGTCTCTAG

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 14857.70 Da
• Isoelectric Point: 4.3147

>AT44996 WP_010518062.1 NZ_CP004364:c39533-39117 [Komagataeibacter xylinus E25]
MRYPVVIERGSETTAFGVVFPDLPGCFSAGDTLDEAISNAEEAAAAWIDATLDAGEAVPSPSTLDAIQNNPDYEGWTVGF
VTIDPAVLDDRVERANISLPRRVLERLDALARSSHESRSGMIAAMTLQARLKPVVRSK

Nucleotide

• Length: 417 bp

>AT44996 NZ_CP004364:c39533-39117 [Komagataeibacter xylinus E25]
ATGCGCTATCCGGTTGTTATCGAACGGGGCAGCGAGACAACGGCATTTGGCGTTGTCTTTCCTGACCTCCCCGGCTGCTT
TTCGGCAGGCGACACGCTCGATGAAGCCATTTCCAATGCGGAAGAGGCCGCTGCGGCATGGATCGACGCCACGCTGGATG
CCGGAGAGGCTGTGCCTTCTCCCAGCACGCTCGACGCCATCCAGAACAACCCGGATTATGAGGGCTGGACAGTGGGCTTT
GTCACCATTGATCCGGCCGTCCTCGATGACCGGGTGGAACGGGCGAACATCTCCCTTCCCCGGCGCGTTCTGGAGCGGCT
GGATGCGCTTGCCAGATCATCCCATGAGAGCCGTTCCGGCATGATCGCGGCCATGACCCTGCAGGCAAGGCTTAAGCCGG
TGGTCAGGAGCAAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0D6QDJ4