Detailed information of TA system TA044995

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PemK-MazE
• Location: 36255..36793
• Replicon: plasmid pGX4
• Accession: NZ_CP004364
• Organism: Komagataeibacter xylinus E25

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: W5YKZ7
• Locus tag: H845_RS16110
• Protein ID: WP_025439965.1
• Coordinates: 36255..36578 (-)
• Length: 108 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: W5YE56
• Locus tag: H845_RS16115
• Protein ID: WP_025439966.1
• Coordinates: 36575..36793 (-)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H845_RS20180	31608..33518	+	1911	Protein_41	phospholipase D family protein	-
H845_RS16095	33604..34626	+	1023	WP_041247965.1	IS110 family transposase	-
H845_RS19945	34904..35047	+	144	WP_157699282.1	hypothetical protein	-
H845_RS16100	35091..35477	-	387	WP_025439964.1	type II toxin-antitoxin system VapC family toxin	-
H845_RS16105	35474..35719	-	246	WP_010518373.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
H845_RS18925	35740..36000	-	261	WP_034931139.1	type II toxin-antitoxin system HicA family toxin	-
H845_RS20185	36073..36225	+	153	WP_165165566.1	hypothetical protein	-
H845_RS16110	36255..36578	-	324	WP_025439965.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
H845_RS16115	36575..36793	-	219	WP_025439966.1	antitoxin MazE family protein	Antitoxin
H845_RS18930	37029..37355	-	327	WP_117125944.1	DUF2442 domain-containing protein	-
H845_RS16120	37448..37963	-	516	WP_010518057.1	hypothetical protein	-
H845_RS20190	37869..38186	-	318	WP_167331481.1	hypothetical protein	-
H845_RS16130	38249..38434	-	186	WP_010518059.1	PilT protein	-
H845_RS16135	38431..38688	-	258	WP_019087450.1	plasmid stabilization protein	-
H845_RS16140	38786..39040	-	255	WP_081749535.1	hypothetical protein	-
H845_RS16145	39117..39533	-	417	WP_010518062.1	type II toxin-antitoxin system HicB family antitoxin	-
H845_RS18935	39544..39723	-	180	WP_010518064.1	type II toxin-antitoxin system HicA family toxin	-
H845_RS18940	40500..41615	-	1116	WP_167331479.1	IS110 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..87176	87176

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (2-103)

Antitoxin

• Antitoxin MazE-like (8-70)

Sequences

Toxin

Protein

• Length: 108 a.a.
• Molecular weight: 11564.62 Da
• Isoelectric Point: 8.8084

>T44995 WP_025439965.1 NZ_CP004364:c36578-36255 [Komagataeibacter xylinus E25]
MKRGDFVTVVLQDDLGKPQPALVVQADRFDMTAVVTLLPLSSALVDAPLLRLTIRPDEHNGLRKPSQVMIDKPTTINREK
VGPAFGIASDTLMLSVSRALALFLGLA

Nucleotide

• Length: 324 bp

>T44995 NZ_CP004364:c36578-36255 [Komagataeibacter xylinus E25]
ATGAAGCGAGGCGACTTTGTAACCGTTGTCCTGCAGGACGATCTCGGCAAACCCCAGCCTGCATTGGTGGTGCAGGCTGA
TCGGTTCGACATGACAGCTGTCGTGACCCTCCTGCCCCTCTCCAGCGCCCTTGTGGACGCTCCCCTGCTTCGCCTGACAA
TCCGGCCTGATGAGCACAATGGACTACGTAAGCCATCACAGGTCATGATCGACAAGCCGACCACCATCAACCGCGAGAAG
GTTGGCCCGGCTTTCGGGATCGCCAGCGATACCCTCATGCTGTCTGTCAGTCGGGCGCTCGCCCTTTTCCTCGGACTGGC
GTAG

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8038.23 Da
• Isoelectric Point: 5.7925

>AT44995 WP_025439966.1 NZ_CP004364:c36793-36575 [Komagataeibacter xylinus E25]
MTPVAICVKKRRDTLRKAGLRPVQIWVPDTRAKGFDEECRRQAMLVALADTHEPDIASFLDTAAADLDGWEA

Nucleotide

• Length: 219 bp

>AT44995 NZ_CP004364:c36793-36575 [Komagataeibacter xylinus E25]
ATGACCCCTGTTGCCATTTGCGTAAAGAAGCGTCGAGACACGCTCCGCAAGGCTGGACTGCGCCCTGTCCAAATCTGGGT
TCCCGATACACGGGCAAAGGGGTTTGACGAGGAATGCCGCCGGCAGGCCATGCTGGTAGCCCTGGCTGACACGCATGAGC
CAGATATTGCCTCTTTCCTCGATACCGCTGCCGCCGATCTGGATGGCTGGGAGGCATGA

Structures

Toxin

• AlphaFold DB: W5YKZ7

Antitoxin

• AlphaFold DB: W5YE56