Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VapB
Location 19911..20592 Replicon plasmid pGX4
Accession NZ_CP004364
Organism Komagataeibacter xylinus E25

Toxin (Protein)


Gene name vapC Uniprot ID W5YKX3
Locus tag H845_RS16010 Protein ID WP_025439952.1
Coordinates 20170..20592 (-) Length 141 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID W5YD93
Locus tag H845_RS16005 Protein ID WP_010512461.1
Coordinates 19911..20105 (-) Length 65 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H845_RS15975 15088..15720 - 633 WP_157699281.1 invasion associated locus B family protein -
H845_RS15980 16382..16651 + 270 WP_025439948.1 hypothetical protein -
H845_RS15985 16651..17058 + 408 WP_025439949.1 Rrf2 family transcriptional regulator -
H845_RS15990 17152..18246 - 1095 WP_025439950.1 Fic family protein -
H845_RS15995 18561..19229 - 669 WP_025439951.1 hypothetical protein -
H845_RS16000 19522..19914 - 393 WP_007396299.1 PIN domain nuclease -
H845_RS16005 19911..20105 - 195 WP_010512461.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
H845_RS16010 20170..20592 - 423 WP_025439952.1 type II toxin-antitoxin system VapC family toxin Toxin
H845_RS16015 20589..20840 - 252 WP_025439953.1 plasmid stabilization protein -
H845_RS16020 20926..21189 - 264 WP_041250516.1 toxin-antitoxin system HicB family antitoxin -
H845_RS16025 21186..21608 - 423 WP_010517487.1 putative toxin-antitoxin system toxin component, PIN family -
H845_RS16030 21655..25026 - 3372 WP_025439955.1 Ti-type conjugative transfer relaxase TraA -
H845_RS16035 25123..25452 + 330 WP_081749532.1 conjugal transfer protein TraD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..87176 87176


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(2-122)

Antitoxin

Bacterial antitoxin of type II TA system, VapB

(1-47)


Sequences

Toxin        


Download         Length: 141 a.a.        Molecular weight: 15256.50 Da        Isoelectric Point: 5.2273

>T44993 WP_025439952.1 NZ_CP004364:c20592-20170 [Komagataeibacter xylinus E25]
MILLDTNVISEPWKPVPEPRVLAWIDGQAIETLFLSAVTVAELRFGIGAMPTGRRRSVLHERLEQEVLPLFAGRVLSFDL
AASQAYAELMVRARSEGRAIGKADGYIAATAASRGYVVASRDVSSFEAAGVRVLNPWEDT

Download         Length: 423 bp

>T44993 NZ_CP004364:c20592-20170 [Komagataeibacter xylinus E25]
ATGATCCTGCTCGATACCAATGTGATCTCGGAGCCGTGGAAGCCCGTCCCGGAGCCGCGTGTTCTGGCCTGGATCGATGG
ACAGGCGATCGAGACGCTTTTCCTGTCTGCCGTCACGGTGGCGGAACTGCGTTTCGGGATCGGGGCGATGCCGACCGGTC
GGCGGCGTTCTGTACTGCATGAGCGGCTGGAACAGGAGGTATTGCCCTTGTTCGCGGGGCGTGTCCTGTCGTTCGATCTG
GCGGCGTCCCAGGCCTATGCCGAACTGATGGTGCGGGCCAGGTCAGAGGGTCGGGCGATTGGCAAGGCAGATGGCTATAT
TGCCGCGACTGCCGCATCCCGTGGTTATGTCGTGGCCAGTCGCGACGTGTCGTCTTTCGAGGCGGCTGGTGTGCGGGTTC
TCAATCCGTGGGAGGATACCTGA

Antitoxin


Download         Length: 65 a.a.        Molecular weight: 7268.49 Da        Isoelectric Point: 10.4776

>AT44993 WP_010512461.1 NZ_CP004364:c20105-19911 [Komagataeibacter xylinus E25]
MRTNIIIDDALMTDALKASGVKTKKEAVELGLRTLIRLKQQRELRTLRGKLDWQGDLDAMRSDA

Download         Length: 195 bp

>AT44993 NZ_CP004364:c20105-19911 [Komagataeibacter xylinus E25]
ATGCGAACAAACATCATCATTGACGATGCCCTCATGACAGATGCGCTGAAGGCGTCTGGTGTCAAAACCAAGAAGGAAGC
GGTCGAACTCGGGTTGCGGACGCTGATCAGGCTGAAGCAGCAGCGGGAACTCCGTACCTTGCGGGGGAAGCTCGACTGGC
AGGGTGATCTGGATGCCATGCGTTCCGACGCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB W5YKX3

Antitoxin

Source ID Structure
AlphaFold DB A0A318QDR0

References