Detailed information of TA system TA044991

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 165625..166217
• Replicon: chromosome
• Accession: NZ_CP004360
• Organism: Komagataeibacter xylinus E25

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: W5Y5C8
• Locus tag: H845_RS00660
• Protein ID: WP_019087476.1
• Coordinates: 165963..166217 (-)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: W5YC50
• Locus tag: H845_RS00655
• Protein ID: WP_025437223.1
• Coordinates: 165625..165966 (-)
• Length: 114 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H845_RS00635	161564..162775	+	1212	WP_025437219.1	integrase arm-type DNA-binding domain-containing protein	-
H845_RS00640	163085..163405	+	321	WP_081749484.1	HigA family addiction module antidote protein	-
H845_RS00645	163580..164623	+	1044	WP_025437221.1	Lrp/AsnC family transcriptional regulator	-
H845_RS00655	165625..165966	-	342	WP_025437223.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
H845_RS00660	165963..166217	-	255	WP_019087476.1	type II toxin-antitoxin system HicA family toxin	Toxin
H845_RS00665	166430..167998	-	1569	WP_025437224.1	IS66 family transposase	-
H845_RS00670	168064..168411	-	348	WP_019092242.1	IS66 family insertion sequence element accessory protein TnpB	-
H845_RS00675	168408..168791	-	384	WP_081749485.1	transposase	-
H845_RS00680	169383..170246	-	864	WP_025437226.1	NgoMIV family type II restriction endonuclease	-
H845_RS00685	170246..171202	-	957	WP_025437227.1	DNA cytosine methyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	168064..168411	347

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (23-81)

Antitoxin

• Uncharacterised protein family HicB (55-105)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 9431.88 Da
• Isoelectric Point: 10.7588

>T44991 WP_019087476.1 NZ_CP004360:c166217-165963 [Komagataeibacter xylinus E25]
MKAKHRRTLELIFKRPVSGNVPWSDIEALFVSLGAEVSEREGSRIGVVLFGEIRVFHRPHPKPDTDKGAVASVRKWLEQN
GVTP

Nucleotide

• Length: 255 bp

>T44991 NZ_CP004360:c166217-165963 [Komagataeibacter xylinus E25]
ATGAAAGCCAAGCATCGCCGCACCCTCGAACTGATCTTCAAGCGCCCTGTCTCCGGCAATGTGCCGTGGAGCGATATTGA
AGCCCTGTTCGTCTCGCTGGGTGCCGAGGTGTCGGAGCGGGAAGGATCGCGCATTGGCGTGGTCCTGTTCGGGGAAATCA
GGGTCTTTCATCGGCCCCACCCCAAGCCGGATACGGACAAGGGTGCGGTGGCATCGGTGCGAAAATGGCTGGAGCAGAAC
GGAGTTACGCCATGA

Antitoxin

Protein

• Length: 114 a.a.
• Molecular weight: 12564.18 Da
• Isoelectric Point: 4.5408

>AT44991 WP_025437223.1 NZ_CP004360:c165966-165625 [Komagataeibacter xylinus E25]
MNNMMEIGGHRAVIQFDPEIGMFRGEFLGLNGGADFYADSVEGLRKEGETSLRVFLEMCEEKGIEPTKRYSGKFQVRLSP
DEHARAAEIAAARGVSLNRLVQDALEQVEGEPF

Nucleotide

• Length: 342 bp

>AT44991 NZ_CP004360:c165966-165625 [Komagataeibacter xylinus E25]
ATGAACAACATGATGGAGATTGGCGGCCACCGGGCCGTCATCCAGTTTGACCCGGAAATCGGCATGTTCCGGGGAGAGTT
CCTTGGCCTGAATGGTGGGGCGGACTTCTATGCCGACAGCGTGGAAGGGTTGCGGAAGGAAGGCGAGACTTCGCTCCGCG
TGTTTCTGGAGATGTGCGAGGAAAAAGGGATCGAACCTACGAAACGCTACTCTGGCAAGTTTCAGGTCCGCCTGTCGCCA
GATGAGCACGCCCGTGCGGCTGAGATCGCTGCTGCCCGTGGCGTTAGCCTCAACCGGCTTGTCCAGGATGCATTGGAACA
GGTTGAGGGCGAGCCGTTTTGA

Structures

Toxin

• AlphaFold DB: W5Y5C8

Antitoxin

• AlphaFold DB: W5YC50