Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicA-HicB
Location 713477..714116 Replicon chromosome
Accession NZ_CP004118
Organism Burkholderia thailandensis E444

Toxin (Protein)


Gene name hicA Uniprot ID Q2T3Q6
Locus tag BTJ_RS19895 Protein ID WP_009893919.1
Coordinates 713937..714116 (-) Length 60 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID Q2T3Q7
Locus tag BTJ_RS19890 Protein ID WP_011401461.1
Coordinates 713477..713893 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BTJ_RS19880 710573..711307 + 735 WP_009893914.1 hypothetical protein -
BTJ_RS31330 711360..711932 + 573 WP_009893915.1 site-specific integrase -
BTJ_RS19885 712551..713048 + 498 WP_011401460.1 hypothetical protein -
BTJ_RS19890 713477..713893 - 417 WP_011401461.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
BTJ_RS19895 713937..714116 - 180 WP_009893919.1 type II toxin-antitoxin system HicA family toxin Toxin
BTJ_RS19900 714396..714923 + 528 WP_011401462.1 PAAR domain-containing protein -
BTJ_RS19905 714920..715654 + 735 WP_011401463.1 VRR-NUC domain-containing protein -
BTJ_RS19910 715664..716779 + 1116 WP_009893924.1 DUF3396 domain-containing protein -
BTJ_RS19915 716850..717425 - 576 WP_009893925.1 recombinase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element - ddhA 657518..740438 82920


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(4-59)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(3-126)


Sequences

Toxin        


Download         Length: 60 a.a.        Molecular weight: 6618.82 Da        Isoelectric Point: 11.2336

>T44990 WP_009893919.1 NZ_CP004118:c714116-713937 [Burkholderia thailandensis E444]
MNSSKLIRMLEEDGWRLVRVTGSHHHFKHPKKPGLVTVPHPKKDLPIGTVKSIQKSAGL

Download         Length: 180 bp

>T44990 NZ_CP004118:c714116-713937 [Burkholderia thailandensis E444]
ATGAACTCATCGAAGCTGATCCGGATGCTTGAGGAAGATGGCTGGAGGTTGGTTCGAGTGACAGGCAGCCATCATCATTT
CAAACACCCCAAGAAGCCAGGTCTTGTGACGGTTCCCCACCCGAAGAAGGACCTACCGATTGGGACTGTAAAAAGCATCC
AGAAATCCGCCGGCCTGTGA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15106.03 Da        Isoelectric Point: 4.6944

>AT44990 WP_011401461.1 NZ_CP004118:c713893-713477 [Burkholderia thailandensis E444]
MEFPIAVHKDDGSVYGVTVPDIPGVHSWGETIDDAIKNTREAIVGHVETLIELGDDVEFTCSTVEELVAKPEYAGAVWAL
VSVDLSQLDSKPERINVSIPRFVLHKIDAYVASRHETRSGFLARAALEALNEGKVRHA

Download         Length: 417 bp

>AT44990 NZ_CP004118:c713893-713477 [Burkholderia thailandensis E444]
ATGGAATTTCCCATCGCAGTGCATAAGGACGATGGGAGCGTCTACGGCGTGACGGTCCCGGATATTCCGGGCGTTCACTC
CTGGGGCGAAACCATCGATGATGCAATCAAAAACACGAGAGAGGCTATCGTCGGCCATGTAGAGACATTGATCGAGCTTG
GGGACGATGTTGAATTCACTTGCTCGACGGTGGAAGAACTGGTTGCAAAGCCCGAGTACGCCGGGGCGGTTTGGGCGCTC
GTCAGCGTTGATCTTTCTCAGCTTGATTCCAAGCCCGAGCGGATCAATGTGAGTATCCCTCGCTTCGTGCTGCACAAGAT
TGACGCCTATGTGGCATCCCGCCACGAGACTAGAAGCGGATTTCTTGCGCGTGCTGCCCTGGAGGCACTTAATGAAGGCA
AAGTTAGGCACGCATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4C26
PDB 6G26

Antitoxin

Source ID Structure
AlphaFold DB Q2T3Q7

References