Detailed information of TA system TA044984

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT_toxin-BrnA
• Location: 1249780..1250317
• Replicon: chromosome
• Accession: NZ_CP004098
• Organism: Burkholderia thailandensis 2002721723

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: Q2T6G2
• Locus tag: BTQ_RS22080
• Protein ID: WP_004549731.1
• Coordinates: 1249780..1250049 (+)
• Length: 90 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: Q2T6G1
• Locus tag: BTQ_RS22085
• Protein ID: WP_011401115.1
• Coordinates: 1250033..1250317 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BTQ_RS22040	1245157..1245978	+	822	WP_009896491.1	hypothetical protein	-
BTQ_RS22045	1245975..1246235	+	261	WP_004526650.1	helix-turn-helix domain-containing protein	-
BTQ_RS22050	1246389..1247381	+	993	WP_015967393.1	hypothetical protein	-
BTQ_RS22055	1247378..1247929	+	552	WP_011401112.1	DUF1064 domain-containing protein	-
BTQ_RS22060	1247926..1248282	+	357	WP_009896497.1	hypothetical protein	-
BTQ_RS31585	1248722..1249054	+	333	WP_011401114.1	hypothetical protein	-
BTQ_RS22070	1249030..1249290	+	261	WP_009896498.1	hypothetical protein	-
BTQ_RS22075	1249287..1249730	+	444	WP_009896499.1	hypothetical protein	-
BTQ_RS22080	1249780..1250049	+	270	WP_004549731.1	BrnT family toxin	Toxin
BTQ_RS22085	1250033..1250317	+	285	WP_011401115.1	BrnA antitoxin family protein	Antitoxin
BTQ_RS22095	1250948..1251427	+	480	WP_025404174.1	terminase small subunit	-
BTQ_RS22100	1251427..1253169	+	1743	WP_011401117.1	terminase large subunit	-
BTQ_RS22105	1253177..1254472	+	1296	WP_009896509.1	phage portal protein	-
BTQ_RS22110	1254450..1255292	+	843	WP_011401119.1	Clp protease ClpP	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1230348..1274991	44643

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-81)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (27-88)

Sequences

Toxin

Protein

• Length: 90 a.a.
• Molecular weight: 10157.46 Da
• Isoelectric Point: 5.6831

>T44984 WP_004549731.1 NZ_CP004098:1249780-1250049 [Burkholderia thailandensis 2002721723]
MDITFDPTKNETNIAKHGVSLALAAQLDWSDVLSYVDDRRDYSEVREVGFGVIGDRLYCVVFTQRGDSMHIISMRKANKR
EVKSYVEQA

Nucleotide

• Length: 270 bp

>T44984 NZ_CP004098:1249780-1250049 [Burkholderia thailandensis 2002721723]
ATGGACATCACCTTTGACCCGACCAAGAACGAAACGAACATTGCCAAGCATGGAGTGTCGTTGGCGCTTGCAGCGCAACT
CGACTGGTCGGACGTGTTGTCCTACGTGGACGACCGACGCGACTACAGTGAAGTGCGCGAAGTCGGGTTCGGTGTGATCG
GCGATCGCCTCTATTGCGTGGTGTTCACGCAGCGCGGCGACTCGATGCACATCATCAGCATGCGTAAGGCGAACAAGCGG
GAGGTCAAGAGCTATGTCGAGCAAGCGTAA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10669.09 Da
• Isoelectric Point: 5.8930

>AT44984 WP_011401115.1 NZ_CP004098:1250033-1250317 [Burkholderia thailandensis 2002721723]
MSSKRKIVMPTDEEDAAINRGIAADPDTFEVPAEDFAKMTRRGKRGRPPLEAPKVQLTVRYDVDIVDAFKATGEGWQTRM
NDALREWLKEHQPA

Nucleotide

• Length: 285 bp

>AT44984 NZ_CP004098:1250033-1250317 [Burkholderia thailandensis 2002721723]
ATGTCGAGCAAGCGTAAGATCGTCATGCCGACGGACGAGGAAGACGCGGCAATCAACCGCGGCATCGCGGCCGATCCCGA
CACGTTCGAAGTGCCGGCGGAAGACTTCGCGAAGATGACGCGGCGAGGCAAGCGTGGTCGTCCGCCGCTCGAAGCGCCCA
AGGTGCAATTGACGGTGCGCTACGACGTCGACATCGTGGACGCCTTCAAGGCAACAGGCGAAGGTTGGCAGACGCGTATG
AATGATGCGTTGCGCGAGTGGCTGAAGGAGCACCAGCCTGCGTGA

Structures

Toxin

• AlphaFold DB: Q2T6G2

Antitoxin

• AlphaFold DB: Q2T6G1