Detailed information of TA system TA044965

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907(toxin)
• Location: 2855430..2856001
• Replicon: chromosome
• Accession: NZ_CP004081
• Organism: Enterococcus faecalis DENG1

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: DENG_RS14345
• Protein ID: WP_002354774.1
• Coordinates: 2855430..2855771 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: R3JGB1
• Locus tag: DENG_RS14350
• Protein ID: WP_002354773.1
• Coordinates: 2855771..2856001 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DENG_RS14340	2851445..2855059	-	3615	WP_002389470.1	DNA-directed RNA polymerase subunit beta	-
DENG_RS14345	2855430..2855771	-	342	WP_002354774.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
DENG_RS14350	2855771..2856001	-	231	WP_002354773.1	hypothetical protein	Antitoxin
DENG_RS14355	2856176..2856391	-	216	WP_002354768.1	zinc ribbon domain-containing protein	-
DENG_RS14360	2856530..2857522	+	993	WP_002354765.1	biotin--[acetyl-CoA-carboxylase] ligase	-
DENG_RS14365	2857786..2858415	-	630	WP_002354763.1	lytic polysaccharide monooxygenase	-
DENG_RS14375	2859108..2860724	+	1617	WP_002381036.1	phosphatase PAP2/LCP family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-112)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 13156.40 Da
• Isoelectric Point: 9.3984

>T44965 WP_002354774.1 NZ_CP004081:c2855771-2855430 [Enterococcus faecalis DENG1]
MNVEKKYIPKKGDIVWIDFDPAAGKEIQKRRPGLVVSRYEFNRKTMFAVICPITSTIKNIPTRYTLPDDIETQGQVVISQ
LKSLDFTERKLSQIEHLPLKDMAKIDQIIEYIF

Nucleotide

• Length: 342 bp

>T44965 NZ_CP004081:c2855771-2855430 [Enterococcus faecalis DENG1]
ATGAACGTTGAAAAGAAGTACATTCCCAAAAAAGGAGATATTGTATGGATTGATTTTGATCCTGCTGCAGGTAAAGAAAT
TCAGAAGAGACGCCCTGGTTTAGTCGTGTCAAGATATGAATTTAATCGGAAAACCATGTTTGCTGTGATTTGCCCGATTA
CTTCCACCATTAAAAATATTCCCACTCGATATACGTTGCCCGATGACATAGAAACCCAAGGACAAGTAGTGATCTCACAA
CTGAAATCTTTGGACTTCACTGAAAGAAAATTAAGTCAAATTGAGCATTTGCCATTAAAAGATATGGCGAAAATTGACCA
AATAATTGAATATATTTTCTAG

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8677.67 Da
• Isoelectric Point: 4.3313

>AT44965 WP_002354773.1 NZ_CP004081:c2856001-2855771 [Enterococcus faecalis DENG1]
MLTTKSRRQGSSVVLTLPTDNGKKPKVDQEYIVMYSDDGTITLVPKIQDPFSEGPEGEYYEKDEWHDLAPEGRELF

Nucleotide

• Length: 231 bp

>AT44965 NZ_CP004081:c2856001-2855771 [Enterococcus faecalis DENG1]
ATGCTAACAACTAAATCAAGAAGACAAGGAAGCTCGGTCGTTTTAACTTTACCCACTGATAATGGTAAAAAACCAAAAGT
CGACCAAGAATATATTGTGATGTATTCTGACGACGGTACAATTACATTAGTCCCTAAAATTCAGGATCCATTTAGTGAAG
GCCCAGAAGGTGAGTATTATGAAAAAGATGAGTGGCATGATCTTGCACCTGAGGGGAGAGAACTTTTTTAA

Structures

Antitoxin

• AlphaFold DB: A0A0M2A812