Detailed information of TA system TA044956

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 1278668..1279273
• Replicon: chromosome
• Accession: NZ_CP004078
• Organism: Paenibacillus sabinae T27

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: X4ZF21
• Locus tag: PSAB_RS05755
• Protein ID: WP_025333625.1
• Coordinates: 1278923..1279273 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: PSAB_RS05750
• Protein ID: WP_175491834.1
• Coordinates: 1278668..1278919 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PSAB_RS05730	1273802..1274380	-	579	WP_025333620.1	TetR/AcrR family transcriptional regulator	-
PSAB_RS05735	1274534..1275769	+	1236	WP_025333621.1	MFS transporter	-
PSAB_RS05740	1275959..1277050	+	1092	WP_025333622.1	outer membrane lipoprotein-sorting protein	-
PSAB_RS05745	1277244..1278446	+	1203	WP_025333623.1	alanine racemase	-
PSAB_RS05750	1278668..1278919	+	252	WP_175491834.1	ribbon-helix-helix protein, CopG family	Antitoxin
PSAB_RS05755	1278923..1279273	+	351	WP_025333625.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
PSAB_RS05760	1279721..1281865	+	2145	WP_084266631.1	RNA-binding transcriptional accessory protein	-
PSAB_RS05765	1282041..1283021	-	981	WP_025333627.1	S-layer homology domain-containing protein	-
PSAB_RS05770	1283233..1283604	-	372	WP_025333628.1	hypothetical protein	-
PSAB_RS05775	1283791..1284261	+	471	WP_025333629.1	RpiB/LacA/LacB family sugar-phosphate isomerase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (2-35)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12794.71 Da
• Isoelectric Point: 5.1284

>T44956 WP_025333625.1 NZ_CP004078:1278923-1279273 [Paenibacillus sabinae T27]
MIVKRGDVFFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIEAASHGFDRDSVILLEQV
RTIDKQRLTDKITHLDDETMKKVDDSLQISLGLIDF

Nucleotide

• Length: 351 bp

>T44956 NZ_CP004078:1278923-1279273 [Paenibacillus sabinae T27]
TTGATCGTAAAACGCGGCGACGTTTTTTTTGCCGACCTTTCGCCAGTAGTGGGCTCGGAGCAAGGCGGGGTAAGGCCCGT
GCTGGTTATTCAGAACGATATTGGCAATCGCTTCAGTCCTACGGTAATTGTTGCGGCAATTACCGCACAAATTCAGAAGG
CCAAGCTGCCCACCCATGTAGAGATTGAAGCGGCGTCCCACGGGTTTGACCGGGATTCCGTCATTCTGCTGGAGCAGGTC
CGGACGATCGACAAGCAGCGCTTGACGGATAAGATCACCCACCTGGATGACGAAACGATGAAAAAGGTGGATGACTCGCT
GCAAATCAGCTTGGGGCTGATTGATTTTTAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9505.98 Da
• Isoelectric Point: 5.1184

>AT44956 WP_175491834.1 NZ_CP004078:1278668-1278919 [Paenibacillus sabinae T27]
MISLPDHLLQEVDGIVQMENSNRSELIRQAMKLYLSERRKRSIRESMQRGYMEMAKINLTMACEAFLAEEDADSTLGRLV
SGV

Nucleotide

• Length: 252 bp

>AT44956 NZ_CP004078:1278668-1278919 [Paenibacillus sabinae T27]
ATGATCAGCTTGCCCGATCATCTCTTGCAGGAGGTGGACGGAATTGTCCAGATGGAAAATTCCAACCGCAGTGAATTGAT
CCGGCAGGCCATGAAGCTGTATCTCAGCGAACGGAGGAAGCGTTCCATCAGGGAGTCCATGCAGCGTGGATACATGGAAA
TGGCCAAGATTAACTTGACCATGGCATGTGAAGCGTTCCTAGCCGAGGAAGATGCAGACAGCACTCTTGGCCGCTTAGTA
AGCGGGGTGTAG

Structures

Toxin

• AlphaFold DB: X4ZF21