Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 3612497..3613128 Replicon chromosome
Accession NZ_CP004057
Organism Shigella flexneri Shi06HN006 isolate Human

Toxin (Protein)


Gene name hicA Uniprot ID A0A822PMR8
Locus tag SFYV_RS19705 Protein ID WP_001259384.1
Coordinates 3612853..3613128 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag SFYV_RS19700 Protein ID WP_000593555.1
Coordinates 3612497..3612856 (-) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
SFYV_RS19675 (3608629) 3608629..3609573 + 945 WP_000947076.1 nickel ABC transporter permease subunit NikB -
SFYV_RS19680 (3609570) 3609570..3610403 + 834 WP_001008973.1 nickel ABC transporter permease subunit NikC -
SFYV_RS19685 (3610403) 3610403..3611167 + 765 WP_001136253.1 nickel import ATP-binding protein NikD -
SFYV_RS19690 (3611164) 3611164..3611970 + 807 WP_000173660.1 nickel import ATP-binding protein NikE -
SFYV_RS19695 (3611976) 3611976..3612377 + 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
SFYV_RS19700 (3612497) 3612497..3612856 - 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
SFYV_RS19705 (3612853) 3612853..3613128 - 276 WP_001259384.1 type II toxin-antitoxin system HicA family toxin Toxin
SFYV_RS19710 (3613187) 3613187..3614311 - 1125 WP_001216257.1 ABC transporter permease -
SFYV_RS19715 (3614311) 3614311..3617046 - 2736 WP_000149170.1 ribosome-associated ATPase/putative transporter RbbA -
SFYV_RS19720 (3617043) 3617043..3618110 - 1068 WP_000361470.1 HlyD family secretion protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-81)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10146.89 Da        Isoelectric Point: 10.3748

>T44954 WP_001259384.1 NZ_CP004057:c3613128-3612853 [Shigella flexneri Shi06HN006]
MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
CDWLLSIGVKP

Download         Length: 276 bp

>T44954 NZ_CP004057:c3613128-3612853 [Shigella flexneri Shi06HN006]
ATGAGAACCAAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATATTTAAAACTCCTGTTCCTCAAGG
GATCAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGCAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
TGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT44954 WP_000593555.1 NZ_CP004057:c3612856-3612497 [Shigella flexneri Shi06HN006]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT44954 NZ_CP004057:c3612856-3612497 [Shigella flexneri Shi06HN006]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCAGCTGTTATTACCTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGTATTCAGGGGCTGCAAAAAGAAGGAG
AGCTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A822PMR8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References