Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 3587464..3588095 Replicon chromosome
Accession NZ_CP004056
Organism Shigella flexneri 2003036 isolate Human

Toxin (Protein)


Gene name hicA Uniprot ID A0A822PMR8
Locus tag SFY_RS19455 Protein ID WP_001259384.1
Coordinates 3587820..3588095 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag SFY_RS19450 Protein ID WP_000593555.1
Coordinates 3587464..3587823 (-) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
SFY_RS19425 3583596..3584540 + 945 WP_000947076.1 nickel ABC transporter permease subunit NikB -
SFY_RS19430 3584537..3585370 + 834 WP_001008973.1 nickel ABC transporter permease subunit NikC -
SFY_RS19435 3585370..3586134 + 765 WP_001136253.1 nickel import ATP-binding protein NikD -
SFY_RS19440 3586131..3586937 + 807 WP_000173660.1 nickel import ATP-binding protein NikE -
SFY_RS19445 3586943..3587344 + 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
SFY_RS19450 3587464..3587823 - 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
SFY_RS19455 3587820..3588095 - 276 WP_001259384.1 type II toxin-antitoxin system HicA family toxin Toxin
SFY_RS19460 3588154..3589278 - 1125 WP_001216257.1 ABC transporter permease -
SFY_RS19465 3589278..3592013 - 2736 WP_000149170.1 ribosome-associated ATPase/putative transporter RbbA -
SFY_RS19470 3592010..3593077 - 1068 WP_000361470.1 HlyD family secretion protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-81)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10146.89 Da        Isoelectric Point: 10.3748

>T44933 WP_001259384.1 NZ_CP004056:c3588095-3587820 [Shigella flexneri 2003036]
MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
CDWLLSIGVKP

Download         Length: 276 bp

>T44933 NZ_CP004056:c3588095-3587820 [Shigella flexneri 2003036]
ATGAGAACCAAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATATTTAAAACTCCTGTTCCTCAAGG
GATCAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGCAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
TGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT44933 WP_000593555.1 NZ_CP004056:c3587823-3587464 [Shigella flexneri 2003036]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT44933 NZ_CP004056:c3587823-3587464 [Shigella flexneri 2003036]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCAGCTGTTATTACCTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGTATTCAGGGGCTGCAAAAAGAAGGAG
AGCTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A822PMR8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References