Detailed information of TA system TA044916

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 1093781..1094582
• Replicon: chromosome
• Accession: NZ_CP004056
• Organism: Shigella flexneri 2003036 isolate Human

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: SFY_RS05845
• Protein ID: WP_038403381.1
• Coordinates: 1094205..1094582 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: D2ACA1
• Locus tag: SFY_RS05840
• Protein ID: WP_001280958.1
• Coordinates: 1093781..1094155 (+)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SFY_RS05790	1089195..1089605	+	411	Protein_1040	helix-turn-helix domain-containing protein	-
SFY_RS05800	1089670..1090367	+	698	Protein_1041	IS1 family transposase	-
SFY_RS27680	1090382..1090813	+	432	Protein_1042	hypothetical protein	-
SFY_RS28685	1090933..1091106	+	174	WP_000088744.1	DUF905 family protein	-
SFY_RS05810	1091206..1092027	+	822	WP_001234584.1	DUF945 domain-containing protein	-
SFY_RS29475	1092027..1092274	+	248	Protein_1045	antirestriction protein	-
SFY_RS05820	1092369..1092842	+	474	WP_001538139.1	antirestriction protein	-
SFY_RS05825	1092858..1093334	+	477	WP_038403376.1	RadC family protein	-
SFY_RS05830	1093397..1093618	+	222	WP_001220314.1	DUF987 domain-containing protein	-
SFY_RS05840	1093781..1094155	+	375	WP_001280958.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
SFY_RS05845	1094205..1094582	+	378	WP_038403381.1	TA system toxin CbtA family protein	Toxin
SFY_RS05850	1094579..1095067	+	489	WP_038403383.1	hypothetical protein	-
SFY_RS05855	1095079..1095276	+	198	WP_038403385.1	DUF957 domain-containing protein	-
SFY_RS05860	1095361..1095926	+	566	Protein_1053	DUF4942 domain-containing protein	-
SFY_RS05865	1095995..1096963	+	969	WP_038404268.1	IS110 family transposase	-
SFY_RS25535	1097080..1097281	-	202	Protein_1055	hypothetical protein	-
SFY_RS25540	1097285..1097566	+	282	Protein_1056	DUF4942 domain-containing protein	-
SFY_RS05880	1098038..1098976	+	939	Protein_1057	glyoxylate/hydroxypyruvate reductase GhrA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	1095995..1096963	968

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14286.32 Da
• Isoelectric Point: 7.8050

>T44916 WP_038403381.1 NZ_CP004056:1094205-1094582 [Shigella flexneri 2003036]
MNTLPDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFVDERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
STCPRSQLINSIDILRARRATGLMTRDNYRTVNNITRGKHPEAKQ

Nucleotide

• Length: 378 bp

>T44916 NZ_CP004056:1094205-1094582 [Shigella flexneri 2003036]
ATGAATACATTACCCGACACACATGTACGGGAGGTATCTCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAT
CCGACTGCTGGACCAGCATTATGGCCTCACACTGAATGACACACCGTTTGTCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTTAATTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCACCTGTCCCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCTCGCCGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCGGGGGAAGCATCCGGAAGCGAAACAATGA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 13793.62 Da
• Isoelectric Point: 6.4651

>AT44916 WP_001280958.1 NZ_CP004056:1093781-1094155 [Shigella flexneri 2003036]
VSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLYYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRYQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPAPATTS

Nucleotide

• Length: 375 bp

>AT44916 NZ_CP004056:1093781-1094155 [Shigella flexneri 2003036]
GTGTCAGACAAACTCTCCGGGATAACTCATCCCGATGACAATCACGACCGCCCCTGGTGGGGGCTTCCCTGCACAGTGAG
GCCCTGTTTTGGCGCTCGTCTGGTGCAAGAGGGTAACCGCCTGTATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGCT
TCAGCGACGCAGATGCATACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGATATCAGCATACCGTCACGCTGTATGCGAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCAGCACCCGCAACCACCTCATAA

Structures

Antitoxin

• AlphaFold DB: A0A0K4PAV2