Detailed information of TA system TA044911

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 1958622..1960375
• Replicon: chromosome
• Accession: NZ_CP004043
• Organism: Burkholderia pseudomallei MSHR146

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: B1H781
• Locus tag: BBN_RS25880
• Protein ID: WP_004525276.1
• Coordinates: 1959023..1960375 (+)
• Length: 451 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: BBN_RS25875
• Protein ID: WP_038730352.1
• Coordinates: 1958622..1959026 (+)
• Length: 135 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BBN_RS25855	1953626..1955155	-	1530	WP_041188330.1	cellulase	-
BBN_RS25860	1955160..1957556	-	2397	WP_051430414.1	cellulose biosynthesis cyclic di-GMP-binding regulatory protein BcsB	-
BBN_RS40355	1958021..1958284	+	264	WP_004199789.1	hypothetical protein	-
BBN_RS25875	1958622..1959026	+	405	WP_038730352.1	helix-turn-helix domain-containing protein	Antitoxin
BBN_RS25880	1959023..1960375	+	1353	WP_004525276.1	type II toxin-antitoxin system HipA family toxin	Toxin
BBN_RS25885	1960532..1960984	-	453	WP_041199101.1	hypothetical protein	-
BBN_RS25890	1961405..1962319	-	915	WP_004198844.1	LysR family transcriptional regulator	-
BBN_RS25895	1962486..1963439	+	954	WP_004187785.1	agmatinase	-
BBN_RS25900	1963716..1964057	+	342	WP_004539420.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (158-406)

Antitoxin

• Cro/C1-type helix-turn-helix domain (22-71)

Sequences

Toxin

Protein

• Length: 451 a.a.
• Molecular weight: 49790.80 Da
• Isoelectric Point: 9.8300

>T44911 WP_004525276.1 NZ_CP004043:1959023-1960375 [Burkholderia pseudomallei MSHR146]
VSARRARATRLHLWMNGLPVGYWEHARDGERLVYFDEWIGDPQGRPLSLSLPFTPGNQPYRGRLVSDYFDNLLPDSEPIR
RRIAMRYRTGGTSAFALLATLGRDCVGALQMLPPDEAPDDIERIRGHALADADIARLLREVTSAPQAGRHAPLDDLRLSI
AGAQEKTALLRHRGRWLLPEGSTPTTHILKLPLGLVGNRRADMRTSVENEWLCARIVAAYGLPVARCDIAQFDDQKALVV
ERFDRRPSRDARWLLRLPQEDMCQATGTSALDKYQADGGPGIETIMEVLAGSEHARDDRRAFFAAQIVFWLLAATDGHAK
NFSIAHLPGNRYRSTPLYDVLSAHPVIGRGANQLPAQRARLAMGVRGKHIHYPLHQIRRRHWIAQGQRVGFAPADVDALI
DTLTARTAGVVDAVSARLPRDFPRDVADAIFSGMLGLSARLAGDAAARAP

Nucleotide

• Length: 1353 bp

>T44911 NZ_CP004043:1959023-1960375 [Burkholderia pseudomallei MSHR146]
GTGAGCGCCCGCCGCGCACGCGCGACGCGCCTGCACCTGTGGATGAACGGCCTGCCCGTCGGCTACTGGGAGCACGCGCG
CGACGGCGAGCGCCTTGTCTACTTCGACGAATGGATCGGCGATCCGCAAGGCCGGCCGCTGTCGCTGTCGCTGCCGTTCA
CGCCGGGCAACCAGCCGTATCGCGGTCGGCTCGTCAGCGATTATTTCGACAACCTGCTGCCCGACAGCGAGCCGATCCGC
CGGCGAATCGCGATGCGCTACCGCACGGGCGGCACGTCCGCGTTCGCGCTGCTCGCGACGCTCGGCCGCGATTGCGTCGG
CGCGCTGCAGATGCTGCCGCCCGACGAAGCGCCGGACGACATCGAACGCATCCGCGGCCACGCGCTCGCCGACGCGGACA
TCGCGCGCCTGCTGCGCGAAGTCACGTCCGCGCCGCAGGCCGGCCGGCACGCGCCGCTCGACGATCTGCGCCTGTCGATC
GCCGGCGCGCAGGAGAAGACCGCGCTGCTGCGCCATCGCGGCCGCTGGCTGCTGCCCGAAGGGAGCACGCCGACCACGCA
CATCCTGAAGCTGCCGCTCGGGCTCGTCGGCAACCGGCGCGCCGACATGCGCACGTCGGTCGAGAACGAATGGCTGTGCG
CGCGGATCGTCGCCGCGTACGGGTTGCCCGTCGCGCGCTGCGACATCGCGCAGTTCGACGATCAGAAAGCGCTCGTCGTC
GAGCGCTTCGACCGCCGGCCGTCGCGCGACGCGCGCTGGCTCCTGCGGCTGCCGCAGGAAGACATGTGCCAGGCAACCGG
CACGTCCGCGCTCGACAAATATCAGGCCGACGGCGGCCCCGGCATCGAGACGATCATGGAAGTGCTCGCCGGCTCCGAAC
ACGCGCGGGACGACCGCCGCGCGTTCTTCGCGGCGCAGATCGTGTTCTGGCTGCTCGCCGCGACCGACGGCCACGCGAAG
AACTTCAGCATCGCGCACCTGCCCGGCAACCGCTACCGTTCGACGCCGCTTTACGACGTGCTGTCCGCGCATCCGGTCAT
CGGCCGGGGCGCGAACCAGTTGCCCGCGCAGCGCGCGCGGCTAGCGATGGGCGTGCGCGGCAAGCACATCCACTATCCGC
TGCACCAGATCCGGCGGCGGCACTGGATCGCGCAGGGCCAGCGCGTCGGCTTCGCGCCCGCCGACGTCGACGCGCTGATC
GACACGCTGACCGCGCGCACCGCGGGCGTCGTCGACGCGGTGTCGGCGCGGCTGCCGCGCGATTTTCCGCGCGACGTCGC
CGATGCGATCTTCAGCGGAATGCTCGGCCTGAGCGCAAGGCTCGCCGGCGACGCGGCCGCGCGCGCGCCATGA

Antitoxin

Protein

• Length: 135 a.a.
• Molecular weight: 14253.55 Da
• Isoelectric Point: 11.7233

>AT44911 WP_038730352.1 NZ_CP004043:1958622-1959026 [Burkholderia pseudomallei MSHR146]
MAILIEHEIKTLGQLRPILRGFRKSAGLTQAMLASRLGVTQQTYAQFEANPASASVERLFKVLRALDIELTLTLTQVYAA
PAGKDKGEAAKTAAGARAGARRAVPPASAPAPSAAGRAPRPARKRAAPKKREDW

Nucleotide

• Length: 405 bp

>AT44911 NZ_CP004043:1958622-1959026 [Burkholderia pseudomallei MSHR146]
ATGGCCATCCTCATCGAGCACGAGATCAAGACACTCGGCCAACTGCGGCCGATTCTGCGCGGCTTCCGCAAATCGGCCGG
ATTGACGCAGGCGATGCTCGCGAGCCGCCTCGGCGTCACGCAGCAGACCTACGCGCAGTTCGAGGCGAACCCGGCGTCGG
CGAGCGTCGAGCGGCTGTTCAAGGTGCTGCGCGCGCTCGACATCGAACTCACGCTCACGCTCACGCAGGTCTACGCCGCG
CCCGCGGGCAAGGACAAGGGCGAGGCTGCGAAGACGGCCGCAGGCGCGCGCGCGGGCGCGCGACGTGCCGTGCCGCCCGC
GTCCGCGCCCGCTCCGAGCGCCGCCGGGCGCGCGCCCCGCCCCGCCCGCAAGCGCGCCGCCCCGAAAAAGCGGGAGGACT
GGTGA

Structures

Toxin

• AlphaFold DB: A0A2K3EM91