Detailed information of TA system TA044909

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT_toxin-BrnA
• Location: 331192..331633
• Replicon: chromosome
• Accession: NZ_CP004043
• Organism: Burkholderia pseudomallei MSHR146

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: -
• Locus tag: BBN_RS19455
• Protein ID: WP_009939402.1
• Coordinates: 331192..331365 (+)
• Length: 58 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: Q63NA3
• Locus tag: BBN_RS19460
• Protein ID: WP_004525420.1
• Coordinates: 331349..331633 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BBN_RS40575	327072..327500	-	429	Protein_256	DDE-type integrase/transposase/recombinase	-
BBN_RS40180	327498..328271	-	774	Protein_257	prohibitin family protein	-
BBN_RS19420	328268..328417	-	150	WP_004537646.1	bacteriophage protein Gp44	-
BBN_RS19425	328673..329254	+	582	WP_004542548.1	hypothetical protein	-
BBN_RS40185	329485..329784	-	300	WP_161788725.1	hypothetical protein	-
BBN_RS19435	329751..329972	-	222	WP_029248167.1	hypothetical protein	-
BBN_RS40580	330108..330239	-	132	WP_009923452.1	bacteriophage protein Gp48	-
BBN_RS19450	330249..330524	-	276	WP_009975384.1	hypothetical protein	-
BBN_RS19455	331192..331365	+	174	WP_009939402.1	BrnT family toxin	Toxin
BBN_RS19460	331349..331633	+	285	WP_004525420.1	BrnA antitoxin family protein	Antitoxin
BBN_RS19465	332278..332661	+	384	Protein_266	DDE-type integrase/transposase/recombinase	-
BBN_RS35365	332624..333052	+	429	Protein_267	IS110 family transposase	-
BBN_RS19470	333279..333395	-	117	Protein_268	IS3 family transposase	-
BBN_RS19475	333661..335121	+	1461	WP_041188148.1	metallophosphoesterase	-
BBN_RS19480	335280..336542	+	1263	WP_009975402.1	c-type cytochrome	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	314277..332661	18384

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (4-49)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (27-88)

Sequences

Toxin

Protein

• Length: 58 a.a.
• Molecular weight: 6691.59 Da
• Isoelectric Point: 7.9950

>T44909 WP_009939402.1 NZ_CP004043:331192-331365 [Burkholderia pseudomallei MSHR146]
MSYVDDRRDYSEVREVGFGVIGDRLYCVVFTQRGDSMHIISMRKANKREVKSYVEQA

Nucleotide

• Length: 174 bp

>T44909 NZ_CP004043:331192-331365 [Burkholderia pseudomallei MSHR146]
TTGTCCTACGTGGACGACCGACGCGACTACAGTGAAGTGCGCGAAGTCGGGTTCGGTGTGATCGGCGATCGCCTCTATTG
CGTGGTGTTCACGCAGCGCGGCGACTCGATGCACATCATCAGCATGCGTAAGGCGAACAAGCGGGAGGTCAAGAGCTATG
TCGAGCAAGCGTAA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10697.10 Da
• Isoelectric Point: 5.8930

>AT44909 WP_004525420.1 NZ_CP004043:331349-331633 [Burkholderia pseudomallei MSHR146]
MSSKRKIVMPTDEEDAAINRGIAADPDTFEVPAEDFAKMTRRGKRGRPPLEAPKVQLTVRYDVDIVDAFKATGEGWQTRM
NDALREWLREHQPA

Nucleotide

• Length: 285 bp

>AT44909 NZ_CP004043:331349-331633 [Burkholderia pseudomallei MSHR146]
ATGTCGAGCAAGCGTAAGATCGTCATGCCGACGGACGAGGAAGACGCGGCAATCAACCGCGGCATCGCGGCCGATCCCGA
CACGTTCGAAGTGCCGGCGGAAGACTTCGCGAAGATGACGCGGCGAGGCAAGCGTGGTCGTCCGCCGCTCGAAGCGCCCA
AGGTGCAATTGACCGTGCGCTATGACGTCGACATTGTCGACGCCTTCAAAGCGACGGGCGAAGGTTGGCAGACGCGCATG
AATGATGCGTTGCGCGAGTGGCTGAGGGAACATCAGCCTGCGTAG

Structures

Antitoxin

• AlphaFold DB: A0A0F6L9S8