Detailed information of TA system TA044908

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT_toxin-BrnA
• Location: 2904177..2904618
• Replicon: chromosome
• Accession: NZ_CP004024
• Organism: Burkholderia pseudomallei MSHR511

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: -
• Locus tag: BBQ_RS31460
• Protein ID: WP_009939402.1
• Coordinates: 2904445..2904618 (-)
• Length: 58 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: Q63NA3
• Locus tag: BBQ_RS29945
• Protein ID: WP_004525420.1
• Coordinates: 2904177..2904461 (-)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BBQ_RS29925	2899268..2900530	-	1263	WP_009975402.1	c-type cytochrome	-
BBQ_RS29930	2900689..2902149	-	1461	WP_041188148.1	metallophosphoesterase	-
BBQ_RS29935	2902415..2902531	+	117	Protein_2352	IS3 family transposase	-
BBQ_RS38600	2902758..2903186	-	429	Protein_2353	IS110 family transposase	-
BBQ_RS38605	2903149..2903532	-	384	Protein_2354	DDE-type integrase/transposase/recombinase	-
BBQ_RS29945	2904177..2904461	-	285	WP_004525420.1	BrnA antitoxin family protein	Antitoxin
BBQ_RS31460	2904445..2904618	-	174	WP_009939402.1	BrnT family toxin	Toxin
BBQ_RS29950	2905286..2905561	+	276	WP_009975384.1	hypothetical protein	-
BBQ_RS40715	2905571..2905702	+	132	WP_009923452.1	bacteriophage protein Gp48	-
BBQ_RS29965	2905838..2906059	+	222	WP_029248167.1	hypothetical protein	-
BBQ_RS40570	2906026..2906325	+	300	WP_161788725.1	hypothetical protein	-
BBQ_RS29975	2906556..2907137	-	582	WP_004542548.1	hypothetical protein	-
BBQ_RS29980	2907393..2907542	+	150	WP_004537646.1	bacteriophage protein Gp44	-
BBQ_RS40575	2907539..2908312	+	774	Protein_2363	prohibitin family protein	-
BBQ_RS40720	2908310..2908738	+	429	Protein_2364	DDE-type integrase/transposase/recombinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2903149..2921533	18384

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (4-49)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (27-88)

Sequences

Toxin

Protein

• Length: 58 a.a.
• Molecular weight: 6691.59 Da
• Isoelectric Point: 7.9950

>T44908 WP_009939402.1 NZ_CP004024:c2904618-2904445 [Burkholderia pseudomallei MSHR511]
MSYVDDRRDYSEVREVGFGVIGDRLYCVVFTQRGDSMHIISMRKANKREVKSYVEQA

Nucleotide

• Length: 174 bp

>T44908 NZ_CP004024:c2904618-2904445 [Burkholderia pseudomallei MSHR511]
TTGTCCTACGTGGACGACCGACGCGACTACAGTGAAGTGCGCGAAGTCGGGTTCGGTGTGATCGGCGATCGCCTCTATTG
CGTGGTGTTCACGCAGCGCGGCGACTCGATGCACATCATCAGCATGCGTAAGGCGAACAAGCGGGAGGTCAAGAGCTATG
TCGAGCAAGCGTAA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10697.10 Da
• Isoelectric Point: 5.8930

>AT44908 WP_004525420.1 NZ_CP004024:c2904461-2904177 [Burkholderia pseudomallei MSHR511]
MSSKRKIVMPTDEEDAAINRGIAADPDTFEVPAEDFAKMTRRGKRGRPPLEAPKVQLTVRYDVDIVDAFKATGEGWQTRM
NDALREWLREHQPA

Nucleotide

• Length: 285 bp

>AT44908 NZ_CP004024:c2904461-2904177 [Burkholderia pseudomallei MSHR511]
ATGTCGAGCAAGCGTAAGATCGTCATGCCGACGGACGAGGAAGACGCGGCAATCAACCGCGGCATCGCGGCCGATCCCGA
CACGTTCGAAGTGCCGGCGGAAGACTTCGCGAAGATGACGCGGCGAGGCAAGCGTGGTCGTCCGCCGCTCGAAGCGCCCA
AGGTGCAATTGACCGTGCGCTATGACGTCGACATTGTCGACGCCTTCAAAGCGACGGGCGAAGGTTGGCAGACGCGCATG
AATGATGCGTTGCGCGAGTGGCTGAGGGAACATCAGCCTGCGTAG

Structures

Antitoxin

• AlphaFold DB: A0A0F6L9S8