Detailed information of TA system TA044902

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT_toxin-BrnA
• Location: 2847735..2848176
• Replicon: chromosome
• Accession: NZ_CP004004
• Organism: Burkholderia pseudomallei NAU20B-16

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: -
• Locus tag: BBS_RS31495
• Protein ID: WP_009939402.1
• Coordinates: 2848003..2848176 (-)
• Length: 58 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: Q63NA3
• Locus tag: BBS_RS29705
• Protein ID: WP_004525420.1
• Coordinates: 2847735..2848019 (-)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BBS_RS29685	2842826..2844088	-	1263	WP_009975402.1	c-type cytochrome	-
BBS_RS29690	2844247..2845707	-	1461	WP_041188148.1	metallophosphoesterase	-
BBS_RS29695	2845973..2846089	+	117	Protein_2295	IS3 family transposase	-
BBS_RS38575	2846316..2846744	-	429	Protein_2296	IS110 family transposase	-
BBS_RS38580	2846707..2847090	-	384	Protein_2297	DDE-type integrase/transposase/recombinase	-
BBS_RS29705	2847735..2848019	-	285	WP_004525420.1	BrnA antitoxin family protein	Antitoxin
BBS_RS31495	2848003..2848176	-	174	WP_009939402.1	BrnT family toxin	Toxin
BBS_RS29710	2848844..2849119	+	276	WP_009975384.1	hypothetical protein	-
BBS_RS40755	2849129..2849260	+	132	WP_009923452.1	bacteriophage protein Gp48	-
BBS_RS29725	2849396..2849617	+	222	WP_029248167.1	hypothetical protein	-
BBS_RS40585	2849584..2849883	+	300	WP_161788725.1	hypothetical protein	-
BBS_RS29735	2850114..2850695	-	582	WP_004542548.1	hypothetical protein	-
BBS_RS29740	2850951..2851100	+	150	WP_004537646.1	bacteriophage protein Gp44	-
BBS_RS40590	2851097..2851870	+	774	Protein_2306	prohibitin family protein	-
BBS_RS40760	2851868..2852296	+	429	Protein_2307	DDE-type integrase/transposase/recombinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2846707..2865091	18384

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (4-49)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (27-88)

Sequences

Toxin

Protein

• Length: 58 a.a.
• Molecular weight: 6691.59 Da
• Isoelectric Point: 7.9950

>T44902 WP_009939402.1 NZ_CP004004:c2848176-2848003 [Burkholderia pseudomallei NAU20B-16]
MSYVDDRRDYSEVREVGFGVIGDRLYCVVFTQRGDSMHIISMRKANKREVKSYVEQA

Nucleotide

• Length: 174 bp

>T44902 NZ_CP004004:c2848176-2848003 [Burkholderia pseudomallei NAU20B-16]
TTGTCCTACGTGGACGACCGACGCGACTACAGTGAAGTGCGCGAAGTCGGGTTCGGTGTGATCGGCGATCGCCTCTATTG
CGTGGTGTTCACGCAGCGCGGCGACTCGATGCACATCATCAGCATGCGTAAGGCGAACAAGCGGGAGGTCAAGAGCTATG
TCGAGCAAGCGTAA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10697.10 Da
• Isoelectric Point: 5.8930

>AT44902 WP_004525420.1 NZ_CP004004:c2848019-2847735 [Burkholderia pseudomallei NAU20B-16]
MSSKRKIVMPTDEEDAAINRGIAADPDTFEVPAEDFAKMTRRGKRGRPPLEAPKVQLTVRYDVDIVDAFKATGEGWQTRM
NDALREWLREHQPA

Nucleotide

• Length: 285 bp

>AT44902 NZ_CP004004:c2848019-2847735 [Burkholderia pseudomallei NAU20B-16]
ATGTCGAGCAAGCGTAAGATCGTCATGCCGACGGACGAGGAAGACGCGGCAATCAACCGCGGCATCGCGGCCGATCCCGA
CACGTTCGAAGTGCCGGCGGAAGACTTCGCGAAGATGACGCGGCGAGGCAAGCGTGGTCGTCCGCCGCTCGAAGCGCCCA
AGGTGCAATTGACCGTGCGCTATGACGTCGACATTGTCGACGCCTTCAAAGCGACGGGCGAAGGTTGGCAGACGCGCATG
AATGATGCGTTGCGCGAGTGGCTGAGGGAACATCAGCCTGCGTAG

Structures

Antitoxin

• AlphaFold DB: A0A0F6L9S8