Detailed information of TA system TA044897

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT_toxin-BrnA
• Location: 696317..696854
• Replicon: chromosome
• Accession: NZ_CP004002
• Organism: Burkholderia pseudomallei NCTC 13178

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: Q2T6G2
• Locus tag: BBJ_RS20830
• Protein ID: WP_004549731.1
• Coordinates: 696317..696586 (+)
• Length: 90 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: Q63NA3
• Locus tag: BBJ_RS20835
• Protein ID: WP_004525420.1
• Coordinates: 696570..696854 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BBJ_RS41480	692313..692741	-	429	Protein_575	DDE-type integrase/transposase/recombinase	-
BBJ_RS41090	692739..693512	-	774	Protein_576	prohibitin family protein	-
BBJ_RS20795	693509..693646	-	138	WP_009971437.1	hypothetical protein	-
BBJ_RS20800	693914..694495	+	582	WP_004542548.1	hypothetical protein	-
BBJ_RS41095	694726..694995	-	270	WP_051453652.1	hypothetical protein	-
BBJ_RS20810	694992..695213	-	222	WP_029248167.1	hypothetical protein	-
BBJ_RS20825	695490..695765	-	276	WP_041220796.1	hypothetical protein	-
BBJ_RS20830	696317..696586	+	270	WP_004549731.1	BrnT family toxin	Toxin
BBJ_RS20835	696570..696854	+	285	WP_004525420.1	BrnA antitoxin family protein	Antitoxin
BBJ_RS20845	697501..697884	+	384	Protein_584	DDE-type integrase/transposase/recombinase	-
BBJ_RS36525	697847..698275	+	429	Protein_585	IS110 family transposase	-
BBJ_RS20850	698502..698618	-	117	Protein_586	IS3 family transposase	-
BBJ_RS20855	698865..700325	+	1461	WP_004547073.1	metallophosphoesterase	-
BBJ_RS20860	700484..701746	+	1263	WP_009975402.1	c-type cytochrome	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	679319..700325	21006

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-81)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (27-88)

Sequences

Toxin

Protein

• Length: 90 a.a.
• Molecular weight: 10157.46 Da
• Isoelectric Point: 5.6831

>T44897 WP_004549731.1 NZ_CP004002:696317-696586 [Burkholderia pseudomallei NCTC 13178]
MDITFDPTKNETNIAKHGVSLALAAQLDWSDVLSYVDDRRDYSEVREVGFGVIGDRLYCVVFTQRGDSMHIISMRKANKR
EVKSYVEQA

Nucleotide

• Length: 270 bp

>T44897 NZ_CP004002:696317-696586 [Burkholderia pseudomallei NCTC 13178]
ATGGACATCACCTTTGACCCGACCAAGAACGAAACGAACATTGCCAAGCATGGAGTGTCGTTGGCGCTTGCAGCGCAACT
CGACTGGTCGGACGTGTTGTCCTACGTGGACGACCGACGCGACTACAGTGAAGTGCGCGAAGTCGGGTTCGGTGTGATCG
GCGATCGCCTCTATTGCGTGGTGTTCACGCAGCGCGGCGACTCGATGCACATCATCAGCATGCGTAAGGCGAACAAGCGG
GAGGTCAAGAGCTATGTCGAGCAAGCGTAA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10697.10 Da
• Isoelectric Point: 5.8930

>AT44897 WP_004525420.1 NZ_CP004002:696570-696854 [Burkholderia pseudomallei NCTC 13178]
MSSKRKIVMPTDEEDAAINRGIAADPDTFEVPAEDFAKMTRRGKRGRPPLEAPKVQLTVRYDVDIVDAFKATGEGWQTRM
NDALREWLREHQPA

Nucleotide

• Length: 285 bp

>AT44897 NZ_CP004002:696570-696854 [Burkholderia pseudomallei NCTC 13178]
ATGTCGAGCAAGCGTAAGATCGTCATGCCGACGGACGAGGAAGACGCGGCAATCAACCGCGGCATCGCGGCCGATCCCGA
CACGTTCGAAGTGCCGGCGGAAGACTTCGCGAAGATGACGCGGCGAGGCAAGCGTGGCCGTCCGCCGCTCGAAGCGCCCA
AGGTGCAATTGACGGTGCGCTATGACGTCGACATTGTCGACGCCTTCAAGGCAACGGGCGAAGGTTGGCAGACGCGTATG
AATGATGCGTTGCGCGAGTGGCTGAGGGAACATCAGCCTGCGTAG

Structures

Toxin

• AlphaFold DB: Q2T6G2

Antitoxin

• AlphaFold DB: A0A0F6L9S8