Detailed information of TA system TA044896

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/upstrm_HI1419-dnstrm_HI1420
• Location: 1230523..1231196
• Replicon: chromosome
• Accession: NZ_CP004001
• Organism: Burkholderia pseudomallei NCTC 13178

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: BBJ_RS05575
• Protein ID: WP_172644706.1
• Coordinates: 1230523..1230837 (+)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: Q63YL3
• Locus tag: BBJ_RS05580
• Protein ID: WP_004202809.1
• Coordinates: 1230840..1231196 (+)
• Length: 119 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BBJ_RS05540	1225882..1226148	+	267	WP_009971787.1	ogr/Delta-like zinc finger family protein	-
BBJ_RS05545	1226145..1226351	+	207	WP_004532731.1	ogr/Delta-like zinc finger family protein	-
BBJ_RS05550	1226441..1227094	+	654	WP_004532770.1	AAA family ATPase	-
BBJ_RS05555	1227328..1227450	+	123	WP_009897078.1	CopG family transcriptional regulator	-
BBJ_RS05560	1227553..1228086	+	534	WP_004532793.1	bacteriophage gp29 protein	-
BBJ_RS05565	1228083..1228820	+	738	WP_004532838.1	VRR-NUC domain-containing protein	-
BBJ_RS05570	1228835..1229950	+	1116	WP_038749743.1	DUF3396 domain-containing protein	-
BBJ_RS05575	1230523..1230837	+	315	WP_172644706.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BBJ_RS05580	1230840..1231196	+	357	WP_004202809.1	putative addiction module antidote protein	Antitoxin
BBJ_RS05585	1231240..1232295	-	1056	WP_038770527.1	phage portal protein	-
BBJ_RS05590	1232292..1234061	-	1770	WP_038771359.1	terminase ATPase subunit family protein	-
BBJ_RS05595	1234205..1235014	+	810	WP_038770529.1	GPO family capsid scaffolding protein	-
BBJ_RS05600	1235048..1236061	+	1014	WP_004532901.1	phage major capsid protein, P2 family	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1215340..1261075	45735

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (9-101)

Antitoxin

• Uncharacterized protein HI1420 (16-94)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11535.40 Da
• Isoelectric Point: 10.2533

>T44896 WP_172644706.1 NZ_CP004001:1230523-1230837 [Burkholderia pseudomallei NCTC 13178]
MGYNSRIFKVLTTPQFDKWLDGLRDPVGSAAINLRIERAKLGNLGQWRAVGDGVNEMKIDVGPGYRAYFVRRGKIIVVVL
CGGDKSTQKKDIKLAKQIAGELED

Nucleotide

• Length: 315 bp

>T44896 NZ_CP004001:1230523-1230837 [Burkholderia pseudomallei NCTC 13178]
ATGGGTTACAATTCTCGCATATTCAAAGTTCTGACGACCCCCCAGTTTGACAAATGGCTTGACGGGCTTCGCGATCCGGT
CGGTAGCGCGGCAATCAACCTGCGCATCGAGCGGGCGAAGCTTGGCAATCTCGGCCAGTGGCGCGCAGTCGGCGACGGCG
TCAACGAAATGAAGATTGATGTGGGGCCGGGATATCGGGCCTACTTCGTGCGGCGCGGAAAAATTATCGTCGTGGTGTTG
TGCGGCGGGGACAAGTCGACGCAGAAGAAGGACATCAAGCTAGCGAAGCAAATCGCTGGCGAACTGGAGGATTGA

Antitoxin

Protein

• Length: 119 a.a.
• Molecular weight: 12585.41 Da
• Isoelectric Point: 9.9470

>AT44896 WP_004202809.1 NZ_CP004001:1230840-1231196 [Burkholderia pseudomallei NCTC 13178]
MKISELAEFDGSKYLKDEETIRHYLAQAFEDGNPRLIQAALGNVAKARGMTALARESGVKREALYRALSEGGNAEFATIM
KVVGALGLHLTVAPAEPAPVPAPATTRARSRVRTAAHA

Nucleotide

• Length: 357 bp

>AT44896 NZ_CP004001:1230840-1231196 [Burkholderia pseudomallei NCTC 13178]
ATGAAAATCAGCGAACTGGCCGAGTTCGACGGCTCGAAGTACCTGAAGGACGAGGAAACGATTCGTCACTACCTGGCGCA
AGCGTTCGAGGATGGAAACCCGCGGCTGATTCAAGCCGCGCTCGGAAACGTCGCGAAAGCGCGCGGCATGACAGCGCTCG
CGCGCGAGTCCGGCGTGAAGCGTGAAGCGCTCTATCGCGCGCTGTCGGAAGGTGGGAACGCGGAATTCGCAACGATCATG
AAAGTTGTGGGCGCGTTGGGGCTGCACCTGACCGTTGCGCCGGCCGAACCTGCGCCGGTGCCCGCGCCGGCAACAACGCG
TGCACGCTCGCGCGTTCGCACGGCTGCGCACGCGTAA

Structures

Antitoxin

• AlphaFold DB: A0A3N4G1D3