Detailed information of TA system TA044895

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ataRT/GNAT-DUF1778
• Location: 259068..259819
• Replicon: plasmid pKP13f
• Accession: NZ_CP004000
• Organism: Klebsiella pneumoniae subsp. pneumoniae Kp13

Toxin (Protein)

• Gene name: ataT
• Uniprot ID: A0A8T3UHS1
• Locus tag: KP13_RS28355
• Protein ID: WP_004883482.1
• Coordinates: 259337..259819 (+)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: A0A8T3UKU4
• Locus tag: KP13_RS28350
• Protein ID: WP_004883484.1
• Coordinates: 259068..259346 (+)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KP13_RS28340	254602..257520	+	2919	Protein_276	Tn3 family transposase	-
KP13_RS28345	257584..258552	+	969	WP_072200942.1	IS5 family transposase	-
KP13_RS28350	259068..259346	+	279	WP_004883484.1	DUF1778 domain-containing protein	Antitoxin
KP13_RS28355	259337..259819	+	483	WP_004883482.1	GNAT family N-acetyltransferase	Toxin
KP13_RS28360	259872..260354	-	483	WP_004196929.1	hypothetical protein	-
KP13_RS28365	260733..262232	-	1500	WP_004196935.1	kinase	-
KP13_RS28370	262260..263993	-	1734	WP_025368656.1	protein phosphatase 2C domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	dfrA15 / cmlA1 / aadA2 / qacE / sul1 / blaCTX-M-2 / sul2 / rmtG / blaTEM-1A / blaOXA-9 / ant(3'')-Ia / aac(6')-Ib / blaSHV-12 / formA	-	1..295493	295493

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (69-145)

Antitoxin

• Vibrio phage ICP1, Orf50 (10-86)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17491.36 Da
• Isoelectric Point: 9.4950

>T44895 WP_004883482.1 NZ_CP004000:259337-259819 [Klebsiella pneumoniae subsp. pneumoniae Kp13]
MGMRPPEPLTPEHNIAEFCCQDQVINEWLKKKALRNHGTGISRVFVVCSENSKRVIAYYCLSSGSVHRNTVTGTYRRNAP
AAIPVIVLGRLAVDAAWAGKGIGAALLKDAIYRTEHIAVQVGVRALLVHALNDEVVAFYTKFGFETSAVNALTLLFPVKV

Nucleotide

• Length: 483 bp

>T44895 NZ_CP004000:259337-259819 [Klebsiella pneumoniae subsp. pneumoniae Kp13]
ATGGGAATGAGACCACCGGAACCACTCACCCCTGAACACAATATTGCTGAATTTTGTTGCCAGGATCAGGTTATTAATGA
GTGGCTGAAGAAGAAGGCGTTGAGAAACCACGGTACGGGAATATCGAGGGTATTCGTTGTCTGTTCTGAAAACTCAAAAA
GGGTAATTGCGTACTACTGCCTGTCATCCGGAAGCGTGCACAGAAACACTGTAACTGGAACCTACCGTCGTAATGCACCT
GCGGCAATACCGGTCATTGTTCTGGGAAGGCTGGCAGTTGATGCTGCCTGGGCAGGAAAGGGGATCGGAGCTGCACTGCT
TAAAGATGCAATATACCGGACGGAACATATCGCAGTTCAGGTCGGTGTAAGGGCGCTTCTCGTTCATGCCTTAAATGACG
AAGTCGTGGCGTTTTACACAAAGTTTGGATTCGAGACCTCTGCAGTCAATGCACTGACATTATTGTTCCCGGTCAAAGTA
TGA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10720.23 Da
• Isoelectric Point: 4.5423

>AT44895 WP_004883484.1 NZ_CP004000:259068-259346 [Klebsiella pneumoniae subsp. pneumoniae Kp13]
MKPDVREAPINIRAKTSQRDLIDMAANLVSKSRTDFMLEAACREAQDILLDQRLFLLDDKQFDTFIEELDAPVTPERQAR
IDNLMNRKSPWE

Nucleotide

• Length: 279 bp

>AT44895 NZ_CP004000:259068-259346 [Klebsiella pneumoniae subsp. pneumoniae Kp13]
ATGAAACCAGACGTTCGTGAGGCCCCGATAAACATCCGGGCGAAAACCTCCCAGCGCGATCTTATCGACATGGCCGCAAA
TCTTGTCTCTAAATCCCGTACTGATTTTATGCTGGAAGCGGCCTGCCGTGAAGCCCAGGACATCCTGTTAGACCAGCGCT
TGTTTCTGCTGGATGATAAGCAGTTTGATACTTTTATCGAAGAGCTTGACGCTCCTGTAACCCCTGAGCGCCAGGCCCGT
ATCGATAACCTGATGAACCGAAAATCACCATGGGAATGA