Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /RES-TIGR02293
Location 187030..187940 Replicon plasmid pKP13f
Accession NZ_CP004000
Organism Klebsiella pneumoniae subsp. pneumoniae Kp13

Toxin (Protein)


Gene name - Uniprot ID A0A5Q9LR54
Locus tag KP13_RS27935 Protein ID WP_004181896.1
Coordinates 187470..187940 (+) Length 157 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID A0A2J4RLY9
Locus tag KP13_RS27930 Protein ID WP_004181895.1
Coordinates 187030..187473 (+) Length 148 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KP13_RS27910 182607..182972 + 366 Protein_189 transposase -
KP13_RS27915 183663..185150 + 1488 WP_004178082.1 group II intron reverse transcriptase/maturase -
KP13_RS27920 185236..185658 + 423 WP_004181893.1 translesion error-prone DNA polymerase V autoproteolytic subunit -
KP13_RS27925 185658..186929 + 1272 WP_025368644.1 translesion error-prone DNA polymerase V subunit UmuC -
KP13_RS27930 187030..187473 + 444 WP_004181895.1 DUF2384 domain-containing protein Antitoxin
KP13_RS27935 187470..187940 + 471 WP_004181896.1 RES family NAD+ phosphorylase Toxin
KP13_RS27940 188050..188310 - 261 WP_004026352.1 hypothetical protein -
KP13_RS27945 188882..190063 - 1182 WP_004883380.1 IS481 family transposase -
KP13_RS27950 190135..190293 + 159 WP_004181898.1 type I toxin-antitoxin system Hok family toxin -
KP13_RS27955 190822..191526 + 705 WP_001067855.1 IS6-like element IS26 family transposase -
KP13_RS27960 191665..191841 - 177 WP_000954592.1 Arm DNA-binding domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid dfrA15 / cmlA1 / aadA2 / qacE / sul1 / blaCTX-M-2 / sul2 / rmtG / blaTEM-1A / blaOXA-9 / ant(3'')-Ia / aac(6')-Ib / blaSHV-12 / formA - 1..295493 295493


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

RES domain

(3-148)

Antitoxin

Antitoxin Xre-like, helix-turn-helix domain

(30-89)


Sequences

Toxin        


Download         Length: 157 a.a.        Molecular weight: 17549.81 Da        Isoelectric Point: 4.6155

>T44894 WP_004181896.1 NZ_CP004000:187470-187940 [Klebsiella pneumoniae subsp. pneumoniae Kp13]
VIFYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDSFTLLSIDVPDELIQSANMD
ELPGNWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHPEFYGIVQKAQQIPFRFDSRLKPDRK

Download         Length: 471 bp

>T44894 NZ_CP004000:187470-187940 [Klebsiella pneumoniae subsp. pneumoniae Kp13]
GTGATCTTCTACAGGCTGACGAAAACAAAGTATCTTTCTACGGCCTGGACCGGATATGGTGCGAAAGAGGCTGGTGGGCG
CTGGAATAGCGTGGGGGTGTCAATGGTATATGTCTCCGAAACGGCATCACTAACGATGCTGGAGACCCTGGTACACCTGC
ATGCGGCACAGATAATGGACTCTTTCACGCTACTGAGTATCGATGTGCCTGATGAACTGATACAAAGCGCAAACATGGAT
GAATTACCGGGCAACTGGGCTGATGAGGATGCGCCTCAGGAACTGGCTGATTACGGAGATGCCTGGTCATTTACCAGGAG
CTCGGTTGCACTGCGTGTTCCCTCAGCCTTATCGCCGGTAGAGTTCAATTATCTGTTAAATCCTGAGCACCCTGAGTTTT
ACGGGATCGTGCAGAAGGCTCAACAGATACCGTTCCGGTTTGATAGTCGTCTTAAACCTGACAGGAAGTGA

Antitoxin


Download         Length: 148 a.a.        Molecular weight: 16536.87 Da        Isoelectric Point: 10.3013

>AT44894 WP_004181895.1 NZ_CP004000:187030-187473 [Klebsiella pneumoniae subsp. pneumoniae Kp13]
MKTFSLSSTPARPQRLWQVAGLNNADGVALLGQINEGLDGKVVNRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDREAAQKWLNEPNRALSWKVPADLMASETGAYEVIKLITRLEHGVYS

Download         Length: 444 bp

>AT44894 NZ_CP004000:187030-187473 [Klebsiella pneumoniae subsp. pneumoniae Kp13]
ATGAAAACTTTTAGTTTGTCCTCAACTCCAGCCAGACCACAGCGCCTCTGGCAGGTTGCCGGGTTAAATAATGCTGATGG
TGTAGCGCTCCTGGGCCAAATCAATGAAGGCCTGGACGGGAAGGTGGTGAACCGGATCACTGACTGGGCCAGAATAACCC
AGAATGACCTGAGAAAAATGTCCGGTATCCCGTCAACCACATTTAGCCGCAGTGTGAAGGCACGTTTTAACCCGGAACAA
AGTGAACGTCTGGTACGGATTATCCGCGTTATTGATCGGGCGGTAGATTTGTTTGAAGGCGACAGGGAAGCCGCACAGAA
GTGGTTGAACGAACCTAACCGGGCATTGAGCTGGAAAGTGCCAGCAGACCTGATGGCATCCGAAACGGGAGCCTATGAGG
TCATTAAGCTGATTACGCGTCTGGAGCATGGGGTGTACTCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A5Q9LR54

Antitoxin

Source ID Structure
AlphaFold DB A0A2J4RLY9

References