Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) stbDE/ParE-DnaT
Location 67063..67585 Replicon plasmid pKP13f
Accession NZ_CP004000
Organism Klebsiella pneumoniae subsp. pneumoniae Kp13

Toxin (Protein)


Gene name stbE Uniprot ID A0A2J4R0S6
Locus tag KP13_RS27345 Protein ID WP_004181778.1
Coordinates 67301..67585 (+) Length 95 a.a.

Antitoxin (Protein)


Gene name stbD Uniprot ID A0A5Q9LM53
Locus tag KP13_RS27340 Protein ID WP_025368601.1
Coordinates 67063..67311 (+) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KP13_RS27320 62272..63093 - 822 WP_004026535.1 hypothetical protein -
KP13_RS27325 63155..63508 - 354 WP_004181774.1 hypothetical protein -
KP13_RS27330 63653..64639 - 987 WP_025368599.1 hypothetical protein -
KP13_RS27335 64973..66772 - 1800 WP_025368600.1 ATP-dependent helicase -
KP13_RS27340 67063..67311 + 249 WP_025368601.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
KP13_RS27345 67301..67585 + 285 WP_004181778.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
KP13_RS27350 67815..69041 + 1227 WP_025368602.1 transposase -
KP13_RS27355 69327..69752 - 426 WP_040120489.1 IS200/IS605 family transposase -
KP13_RS27360 69789..71015 + 1227 WP_025368604.1 transposase -
KP13_RS31265 71081..72207 - 1127 Protein_77 conjugal transfer protein TraG N-terminal domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid dfrA15 / cmlA1 / aadA2 / qacE / sul1 / blaCTX-M-2 / sul2 / rmtG / blaTEM-1A / blaOXA-9 / ant(3'')-Ia / aac(6')-Ib / blaSHV-12 / formA - 1..295493 295493


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(6-84)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 95 a.a.        Molecular weight: 10970.69 Da        Isoelectric Point: 10.6516

>T44893 WP_004181778.1 NZ_CP004000:67301-67585 [Klebsiella pneumoniae subsp. pneumoniae Kp13]
MTYKLAFNESALKEWKKLGHTIREQFKKKLAERLLNPRVPASQLHGRKDQYKIKLRGAGYRLVYSVNDDVVTVTVIGVGK
RENDDIYNLTKHRN

Download         Length: 285 bp

>T44893 NZ_CP004000:67301-67585 [Klebsiella pneumoniae subsp. pneumoniae Kp13]
ATGACCTATAAGCTGGCCTTTAATGAATCAGCTCTGAAAGAATGGAAAAAATTGGGCCATACCATTCGGGAACAATTCAA
AAAGAAGCTCGCTGAGCGCCTGTTGAACCCACGGGTGCCCGCATCGCAACTCCATGGACGCAAAGACCAGTACAAAATAA
AGCTCCGTGGCGCAGGCTACCGACTGGTTTACAGTGTCAACGATGATGTTGTAACGGTCACCGTAATTGGCGTCGGTAAA
CGTGAGAATGATGATATTTACAATTTGACCAAACACAGAAATTAA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 8906.12 Da        Isoelectric Point: 3.8206

>AT44893 WP_025368601.1 NZ_CP004000:67063-67311 [Klebsiella pneumoniae subsp. pneumoniae Kp13]
MAFQVLTTTAASITELKRDPMGTFNAGEGAPVAILNRNEPAFYCVPPALYAQLMELLEDQELGHIADERIGEAVIEVSLD
DL

Download         Length: 249 bp

>AT44893 NZ_CP004000:67063-67311 [Klebsiella pneumoniae subsp. pneumoniae Kp13]
ATGGCATTTCAGGTACTAACCACTACTGCAGCAAGCATCACAGAGCTTAAACGTGATCCTATGGGCACCTTCAATGCAGG
AGAAGGTGCCCCGGTAGCCATACTGAATCGCAACGAACCCGCTTTTTACTGTGTACCGCCTGCTCTGTATGCTCAACTGA
TGGAGCTTCTTGAAGATCAGGAGCTGGGTCATATTGCTGATGAGCGAATTGGTGAGGCGGTAATCGAGGTCAGTCTAGAT
GACCTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2J4R0S6

Antitoxin

Source ID Structure
AlphaFold DB A0A5Q9LM53

References