Detailed information of TA system TA044862

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 17165..17952
• Replicon: plasmid pZM1402301
• Accession: NZ_CP003716
• Organism: Zymomonas mobilis subsp. mobilis str. CP4 = NRRL B-14023

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: A0A806CGA0
• Locus tag: A265_RS08950
• Protein ID: WP_012954728.1
• Coordinates: 17165..17659 (-)
• Length: 165 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: A0A806DAA8
• Locus tag: A265_RS08955
• Protein ID: WP_012817668.1
• Coordinates: 17656..17952 (-)
• Length: 99 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A265_RS08935	12283..14370	+	2088	WP_144081078.1	TonB-dependent receptor	-
A265_RS08940	14374..15630	+	1257	WP_012954726.1	MFS transporter	-
A265_RS08945	15633..17084	+	1452	WP_012954727.1	NAD-dependent succinate-semialdehyde dehydrogenase	-
A265_RS08950	17165..17659	-	495	WP_012954728.1	GNAT family N-acetyltransferase	Toxin
A265_RS08955	17656..17952	-	297	WP_012817668.1	DUF1778 domain-containing protein	Antitoxin
A265_RS08960	18164..18814	+	651	WP_012954729.1	AAA family ATPase	-
A265_RS08965	18844..19104	+	261	WP_012817666.1	ribbon-helix-helix protein, CopG family	-
A265_RS08970	19673..20134	-	462	WP_012954730.1	hypothetical protein	-
A265_RS08975	20149..20658	-	510	WP_023602514.1	hypothetical protein	-
A265_RS08980	21120..21437	+	318	WP_012954732.1	hypothetical protein	-
A265_RS08985	21562..21813	+	252	WP_012954734.1	DUF2312 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..33915	33915

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (43-144)

Antitoxin

• Vibrio phage ICP1, Orf50 (8-97)

Sequences

Toxin

Protein

• Length: 165 a.a.
• Molecular weight: 18093.13 Da
• Isoelectric Point: 10.1331

>T44862 WP_012954728.1 NZ_CP003716:c17659-17165 [Zymomonas mobilis subsp. mobilis str. CP4 = NRRL B-14023]
VTITAPELLSPEHQVDLFQSGVESLDLWLKKRALKNQMTGASRTFVACKNRRVIAYYALASSAITIKSAHGRFRRNMPDP
IPVVVLGRLAIDQSLHNQGVGRAMVRDAALRIIQAADTIGIRGMLVHALSDEAKSFYEHIGFEPSPIDPMMLMVTLADLK
NSIK

Nucleotide

• Length: 495 bp

>T44862 NZ_CP003716:c17659-17165 [Zymomonas mobilis subsp. mobilis str. CP4 = NRRL B-14023]
GTGACAATAACAGCCCCAGAATTATTATCCCCAGAACATCAAGTTGACTTATTTCAATCTGGTGTAGAGAGCCTTGATCT
ATGGCTGAAAAAGCGTGCTTTAAAAAACCAAATGACAGGAGCCTCTCGCACATTTGTAGCCTGTAAAAATCGACGGGTGA
TAGCTTATTATGCTCTAGCATCTAGCGCTATCACTATAAAATCAGCCCATGGACGATTTAGGCGTAATATGCCAGATCCT
ATTCCTGTTGTCGTTTTAGGGCGGCTTGCTATTGATCAATCTTTACATAACCAAGGCGTTGGACGAGCAATGGTAAGGGA
TGCAGCCTTAAGGATAATACAGGCAGCTGATACTATCGGCATTAGAGGTATGCTCGTTCATGCGCTATCTGATGAAGCCA
AATCTTTCTACGAACATATCGGTTTCGAACCGTCTCCAATCGACCCAATGATGTTGATGGTTACATTAGCCGATTTAAAA
AATAGTATAAAATAA

Antitoxin

Protein

• Length: 99 a.a.
• Molecular weight: 11269.03 Da
• Isoelectric Point: 9.8305

>AT44862 WP_012817668.1 NZ_CP003716:c17952-17656 [Zymomonas mobilis subsp. mobilis str. CP4 = NRRL B-14023]
MVATTTISSPNKREALNIRIKPEERDLIDRAAKIQRKSRTDFVLEAACRAAEEALFDQLIITVDPKAYTNFLTRLDMPPR
PNDRLQKTMQTVAPWDKL

Nucleotide

• Length: 297 bp

>AT44862 NZ_CP003716:c17952-17656 [Zymomonas mobilis subsp. mobilis str. CP4 = NRRL B-14023]
ATGGTAGCCACAACAACAATATCCTCACCAAACAAACGAGAAGCGCTGAATATTAGAATTAAACCGGAAGAACGTGATTT
AATAGATCGAGCGGCAAAAATTCAGAGAAAAAGCCGTACTGATTTTGTATTAGAGGCAGCTTGTAGGGCTGCAGAAGAAG
CATTGTTCGATCAATTAATCATTACTGTAGATCCCAAGGCCTATACTAATTTCTTAACACGCCTTGATATGCCACCACGC
CCAAATGATCGGCTGCAAAAAACCATGCAAACGGTAGCTCCGTGGGATAAACTGTGA

Structures

Toxin

• AlphaFold DB: A0A806CGA0

Antitoxin

• AlphaFold DB: A0A806DAA8