Detailed information of TA system TA044838

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 1748538..1749121
• Replicon: chromosome
• Accession: NZ_CP003183
• Organism: Granulibacter bethesdensis strain NIH2.1

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: GBCGDNIH2_RS08050
• Protein ID: WP_025318818.1
• Coordinates: 1748538..1748792 (+)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: Q0BRY7
• Locus tag: GBCGDNIH2_RS08055
• Protein ID: WP_011632219.1
• Coordinates: 1748789..1749121 (+)
• Length: 111 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GBCGDNIH2_RS08040	1743880..1745592	+	1713	WP_025318819.1	relaxase domain-containing protein	-
GBCGDNIH2_RS08045	1745685..1748411	+	2727	Protein_1555	class I SAM-dependent DNA methyltransferase	-
GBCGDNIH2_RS08050	1748538..1748792	+	255	WP_025318818.1	type II toxin-antitoxin system HicA family toxin	Toxin
GBCGDNIH2_RS08055	1748789..1749121	+	333	WP_011632219.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
GBCGDNIH2_RS08060	1749245..1751290	+	2046	WP_025318817.1	DEAD/DEAH box helicase	-
GBCGDNIH2_RS08065	1751283..1752482	+	1200	WP_011632221.1	GIY-YIG nuclease family protein	-
GBCGDNIH2_RS08070	1752614..1753273	+	660	WP_025318816.1	sce7726 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (23-73)

Antitoxin

• Uncharacterised protein family HicB (54-104)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 9450.94 Da
• Isoelectric Point: 10.7342

>T44838 WP_025318818.1 NZ_CP003183:1748538-1748792 [Granulibacter bethesdensis]
MQKRHAKTLEAIFRRPVSGTIRWAEIEALFVALGAEILEREGSRVAVVLFGEVQVFHRPHPSPETDKGAVAAVRRWLENH
GVKP

Nucleotide

• Length: 255 bp

>T44838 NZ_CP003183:1748538-1748792 [Granulibacter bethesdensis]
ATGCAAAAACGGCACGCCAAGACGCTTGAGGCGATCTTCCGCCGCCCCGTTTCAGGCACCATCCGCTGGGCCGAGATTGA
GGCCCTGTTCGTGGCGCTGGGTGCGGAAATTCTGGAGCGGGAAGGCTCGCGCGTGGCCGTGGTGCTGTTTGGCGAGGTGC
AGGTGTTCCACCGCCCGCACCCCTCGCCGGAGACTGACAAGGGTGCCGTGGCGGCGGTTCGCCGCTGGCTGGAAAACCAT
GGGGTGAAGCCATGA

Antitoxin

Protein

• Length: 111 a.a.
• Molecular weight: 12039.66 Da
• Isoelectric Point: 5.1716

>AT44838 WP_011632219.1 NZ_CP003183:1748789-1749121 [Granulibacter bethesdensis]
MNMLEIDGHKAVVQFDPEIGMFRGEFVGLTGGGDFYADSVEGLKREGRVSLQVFLDTCRERGIAPYRAFSGKFNVRIPGE
LHAEAVQVAAARGVSLNELVREALTHELSS

Nucleotide

• Length: 333 bp

>AT44838 NZ_CP003183:1748789-1749121 [Granulibacter bethesdensis]
ATGAATATGTTGGAGATTGATGGCCACAAGGCCGTTGTGCAGTTCGACCCCGAGATCGGCATGTTCCGGGGCGAGTTTGT
GGGGCTGACCGGCGGCGGGGATTTTTACGCCGATAGCGTGGAAGGGCTGAAGCGCGAGGGGCGCGTCTCGCTGCAAGTGT
TTCTGGACACCTGCCGTGAGCGCGGCATTGCGCCTTACCGCGCGTTCAGCGGCAAGTTCAATGTGCGTATTCCGGGCGAG
CTGCACGCCGAGGCCGTGCAGGTAGCGGCAGCGCGCGGGGTAAGCCTGAATGAGCTGGTGCGCGAGGCGCTGACCCATGA
GCTGAGTTCTTGA

Structures

Antitoxin

• AlphaFold DB: Q0BRY7