Detailed information of TA system TA044835

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 1578585..1579179
• Replicon: chromosome
• Accession: NZ_CP003181
• Organism: Granulibacter bethesdensis strain NIH3.1

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: W6I6F6
• Locus tag: GBCGDNIH3_RS07290
• Protein ID: WP_025286817.1
• Coordinates: 1578585..1578767 (+)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: GBCGDNIH3_RS07295
• Protein ID: WP_025286818.1
• Coordinates: 1578784..1579179 (+)
• Length: 132 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GBCGDNIH3_RS07250	1574091..1574444	+	354	WP_025286811.1	hypothetical protein	-
GBCGDNIH3_RS07255	1574437..1574892	+	456	WP_025286812.1	hypothetical protein	-
GBCGDNIH3_RS07260	1574945..1576111	+	1167	WP_025286813.1	hypothetical protein	-
GBCGDNIH3_RS07270	1576346..1576579	-	234	WP_025286814.1	hypothetical protein	-
GBCGDNIH3_RS07275	1576675..1577436	+	762	WP_125918903.1	hypothetical protein	-
GBCGDNIH3_RS07280	1577605..1577784	-	180	WP_072632655.1	hypothetical protein	-
GBCGDNIH3_RS07285	1577836..1578528	+	693	WP_025286816.1	hypothetical protein	-
GBCGDNIH3_RS07290	1578585..1578767	+	183	WP_025286817.1	type II toxin-antitoxin system HicA family toxin	Toxin
GBCGDNIH3_RS07295	1578784..1579179	+	396	WP_025286818.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
GBCGDNIH3_RS07300	1579659..1579952	-	294	WP_072632656.1	Arc family DNA-binding protein	-
GBCGDNIH3_RS07310	1580306..1581055	+	750	WP_081371632.1	phage regulatory protein/antirepressor Ant	-
GBCGDNIH3_RS07315	1581055..1581426	+	372	WP_025286821.1	hypothetical protein	-
GBCGDNIH3_RS07320	1581490..1581822	-	333	WP_025286822.1	type II toxin-antitoxin system HicB family antitoxin	-
GBCGDNIH3_RS07325	1581819..1582073	-	255	WP_025286823.1	type II toxin-antitoxin system HicA family toxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1555282..1600470	45188

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (4-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-128)

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 6733.79 Da
• Isoelectric Point: 11.1367

>T44835 WP_025286817.1 NZ_CP003181:1578585-1578767 [Granulibacter bethesdensis]
MKSDDLIREMKKAGWTEIRSRGSHHIFRHPDHGHSVTVPHPKTDLGKGLVAAIRKQAGLK

Nucleotide

• Length: 183 bp

>T44835 NZ_CP003181:1578585-1578767 [Granulibacter bethesdensis]
ATGAAAAGCGATGACCTGATCAGAGAGATGAAGAAGGCCGGCTGGACCGAAATCCGGTCTCGCGGCTCCCATCACATCTT
TCGGCATCCCGACCATGGACACTCGGTTACGGTGCCTCATCCGAAGACAGACCTCGGAAAAGGTCTGGTCGCCGCCATTC
GCAAGCAGGCCGGTTTGAAATGA

Antitoxin

Protein

• Length: 132 a.a.
• Molecular weight: 14498.28 Da
• Isoelectric Point: 4.1114

>AT44835 WP_025286818.1 NZ_CP003181:1578784-1579179 [Granulibacter bethesdensis]
MRYPIVIERGSDTDAFGVVVPDLPGCFSAGDTFDEAVTNAAEAIALWVEDALDQGREIPHPSLLDDLRQQADWAAPEWIW
GFASVDPALFDDTTERVNITLSRRILARLDSRAREAGETRSGFIAKMALMF

Nucleotide

• Length: 396 bp

>AT44835 NZ_CP003181:1578784-1579179 [Granulibacter bethesdensis]
ATGCGATATCCTATCGTGATTGAACGCGGTTCGGATACGGACGCTTTCGGTGTGGTTGTTCCCGACTTGCCGGGGTGCTT
CTCGGCAGGTGACACGTTTGATGAAGCCGTCACCAATGCCGCAGAGGCCATTGCCCTGTGGGTCGAGGATGCTCTCGACC
AGGGGCGGGAAATTCCTCATCCGTCCTTATTGGACGATCTGCGTCAACAAGCGGACTGGGCCGCCCCGGAATGGATCTGG
GGCTTCGCAAGCGTCGATCCGGCGCTGTTCGATGATACGACAGAGCGTGTGAACATCACACTGTCACGCCGGATACTGGC
CAGGCTGGATAGCAGGGCACGGGAAGCAGGAGAGACGCGCTCGGGGTTCATCGCGAAGATGGCGTTGATGTTTTGA

Structures

Toxin

• AlphaFold DB: W6I6F6