Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 3101838..3102408 Replicon chromosome
Accession NZ_CP002885
Organism Mycobacterium tuberculosis CCDC5180

Toxin (Protein)


Gene name mazF Uniprot ID P71650
Locus tag CFBR_RS14740 Protein ID WP_003414166.1
Coordinates 3101838..3102194 (-) Length 119 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID P0CL61
Locus tag CFBR_RS14745 Protein ID WP_003901465.1
Coordinates 3102178..3102408 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CFBR_RS14720 3097290..3098978 - 1689 WP_003414155.1 alpha/beta hydrolase family protein -
CFBR_RS14725 3098982..3099308 - 327 WP_003414157.1 hypothetical protein -
CFBR_RS14730 3099481..3100068 + 588 WP_003914429.1 DUF3558 family protein -
CFBR_RS14735 3100087..3101736 + 1650 WP_003899485.1 CocE/NonD family hydrolase -
CFBR_RS14740 3101838..3102194 - 357 WP_003414166.1 type II toxin-antitoxin system toxin endoribonuclease MazF9 Toxin
CFBR_RS14745 3102178..3102408 - 231 WP_003901465.1 antitoxin MazE Antitoxin
CFBR_RS14750 3102451..3103494 - 1044 WP_003414172.1 DUF2293 domain-containing protein -
CFBR_RS22310 3103685..3103960 + 276 WP_003911993.1 DUF1778 domain-containing protein -
CFBR_RS22670 3104136..3104390 - 255 WP_003917684.1 hypothetical protein -
CFBR_RS14765 3104538..3104942 + 405 WP_003414181.1 hypothetical protein -
CFBR_RS14770 3104939..3105130 + 192 WP_003414184.1 hypothetical protein -
CFBR_RS14775 3105194..3106483 + 1290 Protein_2894 transposase family protein -
CFBR_RS14780 3106717..3106974 + 258 WP_003899489.1 hypothetical protein -
CFBR_RS14785 3107079..3107390 + 312 WP_003414190.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-113)

Antitoxin

Ribbon-helix-helix protein, CopG

(3-29)


Sequences

Toxin        


Download         Length: 119 a.a.        Molecular weight: 12858.73 Da        Isoelectric Point: 9.1962

>T44825 WP_003414166.1 NZ_CP002885:c3102194-3101838 [Mycobacterium tuberculosis CCDC5180]
VMRRGEIWQVDLDPARGSEANNQRPAVVVSNDRANATATRLGRGVITVVPVTSNIAKVYPFQVLLSATTTGLQVDCKAQA
EQIRSIATERLLRPIGRVSAAELAQLDEALKLHLDLWS

Download         Length: 357 bp

>T44825 NZ_CP002885:c3102194-3101838 [Mycobacterium tuberculosis CCDC5180]
GTGATGCGCCGCGGTGAGATTTGGCAGGTCGATCTCGACCCCGCTCGAGGTAGCGAAGCGAACAACCAGCGCCCCGCCGT
CGTCGTCAGCAACGACCGGGCCAACGCGACCGCCACGCGTCTTGGGCGCGGCGTCATCACCGTCGTGCCGGTGACGAGCA
ACATCGCCAAGGTCTATCCGTTTCAGGTGTTGTTGTCGGCCACCACTACTGGTCTCCAGGTCGACTGCAAGGCGCAGGCC
GAGCAAATCAGATCGATTGCTACCGAGCGGTTGCTCCGGCCAATCGGCCGAGTTTCAGCCGCCGAACTTGCCCAGCTCGA
TGAGGCTTTGAAACTGCATCTCGACTTATGGTCGTAG

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8231.01 Da        Isoelectric Point: 4.1809

>AT44825 WP_003901465.1 NZ_CP002885:c3102408-3102178 [Mycobacterium tuberculosis CCDC5180]
VKLSVSLSDDDVAILDAYVKRAGLPSRSAGLQHAIRVLRYPTLEDDYANAWQEWSAAGDTDAWEQTVGDGVGDAPR

Download         Length: 231 bp

>AT44825 NZ_CP002885:c3102408-3102178 [Mycobacterium tuberculosis CCDC5180]
GTGAAGTTGAGCGTGAGCCTGTCCGATGACGACGTCGCGATCCTGGACGCATATGTGAAACGAGCGGGATTACCATCCCG
CTCCGCCGGCCTGCAGCATGCGATCCGCGTGCTTCGCTACCCCACGCTCGAAGATGACTATGCCAACGCATGGCAAGAAT
GGTCGGCAGCCGGCGACACGGACGCGTGGGAGCAGACCGTCGGCGACGGAGTCGGTGATGCGCCGCGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6L2A
PDB 5HJZ
PDB 6KYT
PDB 6L29
PDB 7DU5
PDB 6KYS

Antitoxin

Source ID Structure
PDB 7DU5
PDB 6KYT
AlphaFold DB A0A7U4FBW3

References