Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN(toxin)
Location 2862506..2863143 Replicon chromosome
Accession NZ_CP002885
Organism Mycobacterium tuberculosis CCDC5180

Toxin (Protein)


Gene name vapC Uniprot ID P0DMV7
Locus tag CFBR_RS13460 Protein ID WP_003413180.1
Coordinates 2862506..2862901 (-) Length 132 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P9WJ44
Locus tag CFBR_RS13465 Protein ID WP_003413183.1
Coordinates 2862898..2863143 (-) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CFBR_RS13425 2857910..2859121 + 1212 WP_003900845.1 alpha/beta hydrolase family protein -
CFBR_RS13430 2859248..2859907 + 660 WP_003902296.1 hypothetical protein -
CFBR_RS13435 2859904..2860566 + 663 WP_003905878.1 hypothetical protein -
CFBR_RS22295 2860563..2860840 + 278 Protein_2625 type II toxin-antitoxin system VapB family antitoxin -
CFBR_RS13440 2860933..2861346 + 414 WP_003413164.1 PIN domain nuclease -
CFBR_RS13445 2861385..2861642 + 258 WP_003413167.1 ribbon-helix-helix domain-containing protein -
CFBR_RS13450 2861639..2862016 + 378 WP_003413174.1 type II toxin-antitoxin system VapC family toxin -
CFBR_RS13455 2862032..2862406 - 375 WP_003413177.1 hypothetical protein -
CFBR_RS13460 2862506..2862901 - 396 WP_003413180.1 type II toxin-antitoxin system toxin 23S rRNA-specific endonuclease VapC20 Toxin
CFBR_RS13465 2862898..2863143 - 246 WP_003413183.1 type II toxin-antitoxin system antitoxin VapB20 Antitoxin
CFBR_RS13470 2863554..2863973 - 420 WP_003413190.1 A24 family peptidase -
CFBR_RS13475 2863985..2864794 - 810 WP_003413193.1 shikimate dehydrogenase -
CFBR_RS13480 2864791..2866044 - 1254 WP_003413196.1 endolytic transglycosylase MltG -
CFBR_RS13485 2866037..2866549 - 513 WP_003413197.1 Holliday junction resolvase RuvX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Endonuclease VapC20-like

(1-129)

Antitoxin

Ribbon-helix-helix protein, CopG

(11-49)


Sequences

Toxin        


Download         Length: 132 a.a.        Molecular weight: 14619.53 Da        Isoelectric Point: 6.9794

>T44820 WP_003413180.1 NZ_CP002885:c2862901-2862506 [Mycobacterium tuberculosis CCDC5180]
MIFVDTSFWAALGNAGDARHGTAKRLWASKPPVVMTSNHVLGETWTLLNRRCGHRAAVAAAAIRLSTVVRVEHVTADLEE
QAWEWLVRHDEREYSFVDATSFAVMRKKGIQNAYAFDGDFSAAGFVEVRPE

Download         Length: 396 bp

>T44820 NZ_CP002885:c2862901-2862506 [Mycobacterium tuberculosis CCDC5180]
ATGATCTTCGTCGACACGTCCTTCTGGGCTGCACTTGGCAACGCCGGCGACGCTCGGCACGGCACCGCGAAGAGGTTGTG
GGCCAGCAAACCGCCCGTGGTGATGACCTCCAACCATGTCCTGGGCGAGACCTGGACGCTGCTCAACCGGCGCTGCGGTC
ACCGCGCGGCGGTTGCCGCCGCTGCAATCCGCTTGAGCACCGTCGTTCGCGTCGAGCACGTAACAGCCGACCTGGAAGAG
CAGGCATGGGAATGGCTTGTACGCCACGACGAGCGAGAGTACTCGTTCGTCGATGCCACGAGCTTCGCGGTGATGCGAAA
GAAAGGCATCCAAAATGCGTATGCCTTCGATGGTGACTTCAGCGCGGCCGGATTTGTCGAGGTAAGACCCGAGTAG

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 9098.33 Da        Isoelectric Point: 5.6007

>AT44820 WP_003413183.1 NZ_CP002885:c2863143-2862898 [Mycobacterium tuberculosis CCDC5180]
MLVAYICHVKRLQIYIDEDVDRALAVEARRRRTSKAALIREYVAEHLRQPGPDPVDAFVGSFVGEADLSASVDDVVYGKH
E

Download         Length: 246 bp

>AT44820 NZ_CP002885:c2863143-2862898 [Mycobacterium tuberculosis CCDC5180]
ATGCTAGTGGCGTATATTTGCCATGTGAAAAGGCTGCAGATCTACATCGACGAAGACGTTGACCGAGCGCTTGCTGTTGA
AGCGCGACGGCGGCGGACGTCGAAGGCGGCGCTGATCCGGGAGTACGTCGCTGAGCACCTCCGGCAGCCGGGCCCAGATC
CGGTCGATGCCTTCGTCGGATCGTTCGTGGGGGAAGCCGACTTGTCCGCGTCCGTCGACGACGTGGTTTACGGAAAGCAC
GAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5WZ4
PDB 5WZF

Antitoxin

Source ID Structure
AlphaFold DB A0A7U4BW31

References