Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 2224873..2225459 Replicon chromosome
Accession NZ_CP002885
Organism Mycobacterium tuberculosis CCDC5180

Toxin (Protein)


Gene name mazF Uniprot ID G0TLY3
Locus tag CFBR_RS10430 Protein ID WP_003410010.1
Coordinates 2224873..2225217 (-) Length 115 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID P0CL58
Locus tag CFBR_RS10435 Protein ID WP_003410014.1
Coordinates 2225211..2225459 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CFBR_RS10395 2220579..2221178 + 600 WP_003910883.1 L-lysine exporter -
CFBR_RS10400 2221594..2222022 + 429 WP_003409992.1 cellulose-binding protein -
CFBR_RS10405 2222248..2222787 + 540 WP_003900446.1 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(37) -
CFBR_RS10410 2223307..2223867 - 561 WP_003410001.1 RES family NAD+ phosphorylase -
CFBR_RS10415 2223864..2224205 - 342 WP_003410003.1 DUF2384 domain-containing protein -
CFBR_RS10420 2224291..2224548 + 258 WP_003410006.1 hypothetical protein -
CFBR_RS10425 2224449..2224784 - 336 WP_003410009.1 dehydrogenase -
CFBR_RS10430 2224873..2225217 - 345 WP_003410010.1 type II toxin-antitoxin system toxin endoribonuclease MazF6 Toxin
CFBR_RS10435 2225211..2225459 - 249 WP_003410014.1 ribbon-helix-helix protein, CopG family Antitoxin
CFBR_RS10440 2225559..2227874 - 2316 WP_025202608.1 cation transporter ATPase CptG -
CFBR_RS10445 2227871..2228143 - 273 WP_003410017.1 DUF1490 family protein -
CFBR_RS10450 2228196..2228552 - 357 WP_003410018.1 Cd(II)/Pb(II)-sensing metalloregulatory transcriptional regulator CmtR -
CFBR_RS10455 2228709..2229476 + 768 WP_003410019.1 hemerythrin domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(5-112)

Antitoxin

Ribbon-helix-helix protein, CopG

(5-38)


Sequences

Toxin        


Download         Length: 115 a.a.        Molecular weight: 12230.12 Da        Isoelectric Point: 9.8887

>T44810 WP_003410010.1 NZ_CP002885:c2225217-2224873 [Mycobacterium tuberculosis CCDC5180]
VVISRAEIYWADLGPPSGSQPAKRRPVLVIQSDPYNASRLATVIAAVITSNTALAAMPGNVFLPATTTRLPRDSVVNVTA
IVTLNKTDLTDRVGEVPASLMHEVDRGLRRVLDL

Download         Length: 345 bp

>T44810 NZ_CP002885:c2225217-2224873 [Mycobacterium tuberculosis CCDC5180]
GTGGTGATTAGTCGTGCCGAGATCTACTGGGCTGACCTCGGGCCGCCATCAGGCAGTCAGCCGGCGAAGCGCCGCCCGGT
GCTCGTAATCCAGTCAGATCCGTACAACGCAAGTCGCCTTGCCACTGTGATCGCAGCGGTGATCACGTCCAATACGGCGC
TGGCGGCAATGCCCGGCAACGTGTTCTTGCCCGCGACCACAACGCGACTGCCACGTGACTCGGTCGTCAACGTCACGGCG
ATTGTCACGCTCAACAAGACTGACCTCACCGACCGAGTTGGGGAGGTGCCAGCGAGCTTGATGCACGAGGTTGACCGAGG
ACTTCGTCGCGTACTGGACCTTTGA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9290.34 Da        Isoelectric Point: 4.3757

>AT44810 WP_003410014.1 NZ_CP002885:c2225459-2225211 [Mycobacterium tuberculosis CCDC5180]
MKTAISLPDETFDRVSRRASELGMSRSEFFTKAAQRYLHELDAQLLTGQIDRALESIHGTDEAEALAVANAYRVLETMDD
EW

Download         Length: 249 bp

>AT44810 NZ_CP002885:c2225459-2225211 [Mycobacterium tuberculosis CCDC5180]
ATGAAGACAGCTATTTCTCTGCCGGATGAGACGTTCGATCGGGTATCGCGGCGTGCGAGTGAGCTCGGCATGAGTCGGTC
CGAGTTCTTCACGAAGGCTGCGCAGCGCTACCTGCACGAGCTGGACGCCCAATTGCTCACGGGCCAGATCGACAGGGCTC
TAGAGAGCATCCATGGCACCGACGAAGCGGAGGCCCTCGCCGTGGCCAACGCATACCGCGTGCTAGAAACCATGGACGAT
GAGTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5HK3
PDB 5HK0
PDB 5HKC

Antitoxin

Source ID Structure
PDB 7WJ0
PDB 7WNR
AlphaFold DB A0A7U4FAW9

References