Detailed information of TA system TA044805

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2180716..2181419
• Replicon: chromosome
• Accession: NZ_CP002885
• Organism: Mycobacterium tuberculosis CCDC5180

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G0TLK7
• Locus tag: CFBR_RS10115
• Protein ID: WP_003409778.1
• Coordinates: 2180716..2181045 (-)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: G0TLK8
• Locus tag: CFBR_RS21375
• Protein ID: WP_003409780.1
• Coordinates: 2181042..2181419 (-)
• Length: 126 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CFBR_RS10095	2177099..2178169	+	1071	WP_003902252.1	epoxide hydrolase EphB	-
CFBR_RS10100	2178166..2178681	+	516	WP_003409718.1	flavin reductase family protein	-
CFBR_RS10105	2178678..2179739	+	1062	WP_003899092.1	3,4-dihydroxy-2-butanone-4-phosphate synthase	-
CFBR_RS10110	2179736..2180506	+	771	WP_003409775.1	SDR family oxidoreductase	-
CFBR_RS10115	2180716..2181045	-	330	WP_003409778.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
CFBR_RS21375	2181042..2181419	-	378	WP_003409780.1	type II toxin-antitoxin system antitoxin MazE5	Antitoxin
CFBR_RS10125	2181416..2182006	-	591	WP_003409784.1	SEC-C domain-containing protein	-
CFBR_RS10130	2182061..2183425	+	1365	WP_003903691.1	HNH endonuclease	-
CFBR_RS10135	2183580..2184032	-	453	WP_003899095.1	lipoprotein	-
CFBR_RS10140	2184096..2184497	+	402	WP_003409869.1	hypothetical protein	-
CFBR_RS10145	2184490..2184672	-	183	WP_003409870.1	hypothetical protein	-
CFBR_RS10150	2184786..2185136	-	351	WP_003409871.1	hypothetical protein	-
CFBR_RS10155	2185147..2186049	-	903	WP_003899097.1	hypothetical protein	-
CFBR_RS10160	2186070..2186261	-	192	WP_003409876.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-103)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 11787.82 Da
• Isoelectric Point: 8.5361

>T44805 WP_003409778.1 NZ_CP002885:c2181045-2180716 [Mycobacterium tuberculosis CCDC5180]
VTALPARGEVWWCEMAEIGRRPVVVLSRDAAIPRLRRALVAPCTTTIRGLASEVVLEPGSDPIPRRSAVNLDSVESVSVA
VLVNRLGRLADIRMRAICTALEVAVDCSR

Nucleotide

• Length: 330 bp

>T44805 NZ_CP002885:c2181045-2180716 [Mycobacterium tuberculosis CCDC5180]
GTGACCGCACTTCCGGCGCGCGGAGAGGTGTGGTGGTGTGAGATGGCTGAGATCGGTCGGCGACCAGTCGTCGTGCTGTC
GCGCGATGCCGCGATCCCTCGGCTGCGACGCGCACTTGTCGCGCCCTGCACCACGACCATCCGAGGGCTAGCCAGTGAGG
TTGTTCTTGAACCCGGTTCCGACCCGATCCCGCGCCGTTCCGCGGTGAATTTGGACTCAGTCGAAAGTGTCTCGGTCGCG
GTATTGGTGAATCGGCTTGGCCGCCTCGCCGACATCCGGATGCGCGCCATCTGCACGGCCCTCGAGGTCGCCGTCGATTG
CTCTCGATGA

Antitoxin

Protein

• Length: 126 a.a.
• Molecular weight: 13560.07 Da
• Isoelectric Point: 5.1519

>AT44805 WP_003409780.1 NZ_CP002885:c2181419-2181042 [Mycobacterium tuberculosis CCDC5180]
VKTARLQVTLRCAVDLINSSSDQCFARIEHVASDQADPRPGVWHSSGMNRIRLSTTVDAALLTSARDMRAGITDAALIDE
ALAALLARHRSAEVDASYAAYDKHPVDEPDEWGDLASWRRAAGDS

Nucleotide

• Length: 378 bp

>AT44805 NZ_CP002885:c2181419-2181042 [Mycobacterium tuberculosis CCDC5180]
GTGAAGACGGCCCGGTTGCAGGTGACGTTGCGCTGCGCGGTAGACCTCATCAATTCGTCCAGCGACCAATGTTTCGCCCG
TATCGAGCACGTTGCGTCCGATCAGGCGGATCCACGCCCCGGAGTGTGGCATAGTAGTGGCATGAATCGCATACGGCTGA
GCACTACTGTCGACGCCGCACTCTTGACAAGTGCGCGCGACATGCGGGCAGGAATCACTGACGCAGCCCTCATCGATGAG
GCCCTTGCGGCATTACTGGCTCGTCATCGATCGGCCGAGGTGGATGCCAGCTATGCGGCCTACGACAAGCACCCGGTCGA
TGAGCCCGACGAGTGGGGCGATCTAGCGTCGTGGCGGCGGGCGGCTGGAGACTCGTGA

Structures

Toxin

• AlphaFold DB: G0TLK7

Antitoxin

• AlphaFold DB: G0TLK8