Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 1687932..1688548 Replicon chromosome
Accession NZ_CP002885
Organism Mycobacterium tuberculosis CCDC5180

Toxin (Protein)


Gene name mazF Uniprot ID G0TJ74
Locus tag CFBR_RS07935 Protein ID WP_003407593.1
Coordinates 1688231..1688548 (+) Length 106 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G0TJ73
Locus tag CFBR_RS07930 Protein ID WP_003900349.1
Coordinates 1687932..1688234 (+) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CFBR_RS07920 1683818..1685665 + 1848 WP_003407585.1 methylmalonyl-CoA mutase small subunit -
CFBR_RS07925 1685666..1687918 + 2253 WP_003407587.1 methylmalonyl-CoA mutase -
CFBR_RS07930 1687932..1688234 + 303 WP_003900349.1 type II toxin-antitoxin system antitoxin MazE4 Antitoxin
CFBR_RS07935 1688231..1688548 + 318 WP_003407593.1 type II toxin-antitoxin system toxin endoribonuclease MazF4 Toxin
CFBR_RS07940 1688545..1689549 + 1005 WP_003407596.1 methylmalonyl Co-A mutase-associated GTPase MeaB -
CFBR_RS07945 1689602..1690891 + 1290 WP_003902090.1 serine hydrolase -
CFBR_RS07950 1690964..1691737 - 774 WP_003912632.1 class I SAM-dependent methyltransferase -
CFBR_RS07955 1691795..1692007 - 213 WP_003898900.1 dodecin family protein -
CFBR_RS22560 1692017..1692241 - 225 WP_160522526.1 NUDIX domain-containing protein -
CFBR_RS07965 1692511..1693539 + 1029 WP_003407612.1 glycosyltransferase family 2 protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(6-99)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 11377.97 Da        Isoelectric Point: 7.9950

>T44801 WP_003407593.1 NZ_CP002885:1688231-1688548 [Mycobacterium tuberculosis CCDC5180]
VNAPLRGQVYRCDLGYGAKPWLIVSNNARNRHTADVVAVRLTTTRRTIPTWVAMGPSDPLTGYVNADNIETLGKDELGDY
LGEVTPATMNKINTALATALGLPWP

Download         Length: 318 bp

>T44801 NZ_CP002885:1688231-1688548 [Mycobacterium tuberculosis CCDC5180]
GTGAACGCGCCGTTGCGTGGTCAGGTCTATCGATGCGACCTCGGATACGGGGCCAAACCGTGGCTCATCGTCTCCAACAA
CGCCCGCAACCGTCACACCGCCGACGTGGTGGCTGTGCGCCTGACAACAACGCGGAGAACCATACCGACCTGGGTCGCCA
TGGGCCCCAGCGATCCATTGACCGGATACGTCAACGCGGACAACATCGAGACCCTCGGCAAAGACGAGCTCGGTGACTAC
CTCGGTGAGGTCACGCCGGCGACGATGAACAAAATCAACACGGCGCTCGCGACCGCGCTGGGGCTACCGTGGCCATGA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11173.42 Da        Isoelectric Point: 9.5564

>AT44801 WP_003900349.1 NZ_CP002885:1687932-1688234 [Mycobacterium tuberculosis CCDC5180]
VPFLVALSGIISGVRDHSMTVRLDQQTRQRLQDIVKGGYRSANAAIVDAINKRWEALHDEQLDAAYAAAIHDNPAYPYES
EAERSAARARRNARQQRSAQ

Download         Length: 303 bp

>AT44801 NZ_CP002885:1687932-1688234 [Mycobacterium tuberculosis CCDC5180]
GTGCCGTTTCTGGTTGCATTATCCGGTATCATCTCGGGCGTGCGTGATCATTCGATGACCGTGCGGCTCGACCAGCAAAC
TCGCCAGCGCCTGCAAGACATTGTGAAAGGCGGATACCGGAGCGCTAATGCGGCGATCGTCGACGCCATCAACAAGCGCT
GGGAGGCGCTACACGATGAGCAACTCGACGCCGCCTACGCGGCCGCGATCCATGACAATCCGGCGTACCCGTACGAGTCT
GAGGCCGAACGGAGCGCCGCGCGGGCCCGGCGCAACGCCAGGCAGCAGCGCTCGGCACAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5XE3
PDB 5XE2

Antitoxin

Source ID Structure
PDB 5XE3
AlphaFold DB G0TJ73

References