Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 1227675..1228306 Replicon chromosome
Accession NZ_CP002885
Organism Mycobacterium tuberculosis CCDC5180

Toxin (Protein)


Gene name mazF Uniprot ID A0A7U4F9D0
Locus tag CFBR_RS05835 Protein ID WP_003405820.1
Coordinates 1227675..1227986 (-) Length 104 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G0TGZ7
Locus tag CFBR_RS05840 Protein ID WP_003405836.1
Coordinates 1227986..1228306 (-) Length 107 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CFBR_RS05815 1223156..1224580 - 1425 WP_003405805.1 class II fumarate hydratase -
CFBR_RS05820 1224611..1225699 - 1089 WP_003898726.1 class II fructose-bisphosphatase -
CFBR_RS05825 1225755..1226399 + 645 WP_003915494.1 DUF4245 domain-containing protein -
CFBR_RS05830 1226406..1227563 - 1158 WP_003898727.1 AI-2E family transporter -
CFBR_RS05835 1227675..1227986 - 312 WP_003405820.1 type II toxin-antitoxin system toxin endoribonuclease MazF3 Toxin
CFBR_RS05840 1227986..1228306 - 321 WP_003405836.1 type II toxin-antitoxin system antitoxin MazE3 Antitoxin
CFBR_RS21105 1228316..1229841 + 1526 Protein_1143 carboxylesterase/lipase family protein -
CFBR_RS05855 1229859..1230971 - 1113 WP_003405840.1 3 beta-hydroxysteroid dehydrogenase/delta 5-->4-isomerase -
CFBR_RS05860 1230981..1231238 - 258 WP_003405844.1 exodeoxyribonuclease VII small subunit -
CFBR_RS05865 1231228..1232475 - 1248 WP_003405846.1 exodeoxyribonuclease VII large subunit -
CFBR_RS05870 1232472..1233110 - 639 WP_003898730.1 lipid droplet-associated protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(8-85)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 11061.82 Da        Isoelectric Point: 4.9371

>T44795 WP_003405820.1 NZ_CP002885:c1227986-1227675 [Mycobacterium tuberculosis CCDC5180]
MRPIHIAQLDKARPVLILTREVVRPHLTNVTVAPITTTVRGLATEVPVDAVNGLNQPSVVSCDNIQTIPVCDLGRQIGYL
LASQEPALAEAIGNAFDLDWVVA

Download         Length: 312 bp

>T44795 NZ_CP002885:c1227986-1227675 [Mycobacterium tuberculosis CCDC5180]
ATGCGACCTATCCACATCGCACAGCTTGACAAGGCTAGACCCGTCCTGATCCTTACCCGCGAGGTCGTACGTCCGCACCT
CACGAACGTCACCGTCGCCCCGATCACGACAACCGTGCGTGGGCTAGCCACCGAAGTCCCGGTAGACGCGGTGAACGGGC
TGAATCAACCGTCAGTCGTCAGCTGCGACAACATCCAAACGATCCCGGTGTGTGACCTTGGTCGTCAAATCGGCTACCTG
CTCGCGTCGCAGGAGCCGGCTCTAGCCGAGGCCATCGGCAACGCGTTCGACCTCGATTGGGTGGTGGCATAG

Antitoxin


Download         Length: 107 a.a.        Molecular weight: 11394.83 Da        Isoelectric Point: 5.1236

>AT44795 WP_003405836.1 NZ_CP002885:c1228306-1227986 [Mycobacterium tuberculosis CCDC5180]
MYLPWGVVLAGGANGFGAGAYQTGTICEVSTQIAVRLPDEIVAFIDDEVRGQHARSRAAVVLRALERERRRRLAERDAEI
LATNTSATGDLDTLAGHCARTALDID

Download         Length: 321 bp

>AT44795 NZ_CP002885:c1228306-1227986 [Mycobacterium tuberculosis CCDC5180]
ATGTACCTACCCTGGGGGGTCGTCCTCGCCGGCGGCGCGAACGGTTTTGGGGCGGGTGCATACCAAACTGGTACCATCTG
TGAAGTGAGTACACAGATTGCGGTTCGCTTGCCCGATGAGATTGTCGCGTTCATCGACGACGAAGTCCGGGGACAGCATG
CCCGTAGCCGTGCGGCTGTCGTGCTGCGGGCGCTGGAACGGGAACGCCGCCGTCGTCTCGCCGAACGCGACGCGGAGATC
CTCGCCACCAACACGTCAGCCACGGGTGATCTGGATACGCTGGCCGGCCACTGCGCTCGTACCGCCCTCGACATCGACTG
A

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4F9D0

Antitoxin

Source ID Structure
AlphaFold DB G0TGZ7

References