Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN(toxin)
Location 3693942..3694616 Replicon chromosome
Accession NZ_CP002883
Organism Mycobacterium tuberculosis BT1

Toxin (Protein)


Gene name vapC Uniprot ID P9WF52
Locus tag HKBT1_RS17480 Protein ID WP_003417282.1
Coordinates 3693942..3694370 (-) Length 143 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID G0TI59
Locus tag HKBT1_RS17485 Protein ID WP_003417286.1
Coordinates 3694374..3694616 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HKBT1_RS17455 3689764..3690165 - 402 WP_003417264.1 cytidine deaminase -
HKBT1_RS17460 3690306..3690740 + 435 WP_003900017.1 succinate dehydrogenase, cytochrome b556 subunit -
HKBT1_RS17465 3690737..3691171 + 435 WP_003902441.1 succinate dehydrogenase hydrophobic membrane anchor subunit -
HKBT1_RS17470 3691300..3693072 + 1773 WP_003417273.1 succinate dehydrogenase flavoprotein subunit -
HKBT1_RS17475 3693072..3693863 + 792 WP_003417279.1 succinate dehydrogenase iron-sulfur subunit -
HKBT1_RS17480 3693942..3694370 - 429 WP_003417282.1 type II toxin-antitoxin system VapC family toxin Toxin
HKBT1_RS17485 3694374..3694616 - 243 WP_003417286.1 ribbon-helix-helix domain-containing protein Antitoxin
HKBT1_RS17490 3694738..3695352 - 615 WP_003417288.1 class I SAM-dependent methyltransferase -
HKBT1_RS17495 3695349..3696014 - 666 WP_003417290.1 molybdopterin converting factor subunit 1 -
HKBT1_RS17500 3696015..3696548 - 534 WP_003417293.1 cyclic pyranopterin monophosphate synthase MoaC -
HKBT1_RS17505 3696545..3696919 - 375 WP_003417295.1 4a-hydroxytetrahydrobiopterin dehydratase -
HKBT1_RS17510 3697016..3698080 - 1065 WP_003913037.1 GTP 3',8-cyclase MoaA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain toxin

(1-141)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 143 a.a.        Molecular weight: 15834.16 Da        Isoelectric Point: 8.5471

>T44771 WP_003417282.1 NZ_CP002883:c3694370-3693942 [Mycobacterium tuberculosis BT1]
MRALLDVNVLLALLDRDHVDHERARAWITGQIERGWASCAITQNGFVRVISQPRYPSPISVAHAIDLLARATHTRYHEFW
SCTVSILDSKVIDRSRLHSPKQVTDAYLLALAVAHDGRFVTFDQSIALTAVPGATKQHLATL

Download         Length: 429 bp

>T44771 NZ_CP002883:c3694370-3693942 [Mycobacterium tuberculosis BT1]
ATGCGAGCGCTGTTGGACGTCAACGTGCTACTGGCGCTGTTGGACCGCGATCACGTCGACCACGAGCGCGCCCGAGCGTG
GATCACCGGGCAGATCGAGCGGGGATGGGCTTCCTGTGCGATCACCCAAAACGGGTTCGTCCGCGTCATCAGCCAACCGC
GCTATCCCAGCCCAATCTCGGTAGCCCATGCCATCGACTTGTTGGCTCGTGCGACGCACACGCGCTATCACGAGTTCTGG
TCCTGCACCGTCAGCATTCTCGACTCAAAGGTCATCGATCGTTCCCGCCTGCACAGCCCCAAACAGGTCACCGACGCGTA
CTTGCTGGCCCTCGCGGTCGCTCATGATGGCCGATTTGTCACCTTCGATCAGTCGATTGCGTTGACTGCGGTTCCTGGCG
CAACCAAACAGCACCTGGCAACGCTGTAG

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 8849.86 Da        Isoelectric Point: 5.1007

>AT44771 WP_003417286.1 NZ_CP002883:c3694616-3694374 [Mycobacterium tuberculosis BT1]
MRTTLSIDDDVLLAVKERARREKRTAGEILSDLARQALTNQNPQPAASQEDAFHGFEPLPHRGGAVSNALIDRLRDEEAV

Download         Length: 243 bp

>AT44771 NZ_CP002883:c3694616-3694374 [Mycobacterium tuberculosis BT1]
ATGCGCACCACGTTGTCCATCGACGACGATGTATTGCTGGCCGTGAAGGAACGGGCACGCAGGGAGAAGCGCACCGCGGG
TGAAATCCTGTCGGATCTGGCCCGGCAAGCGTTAACCAACCAGAATCCCCAGCCTGCGGCGAGCCAGGAGGACGCCTTTC
ATGGCTTCGAACCCCTGCCTCATCGCGGTGGAGCGGTATCGAACGCGCTGATCGATCGGCTTCGTGACGAGGAGGCCGTG
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4FBL0

Antitoxin

Source ID Structure
AlphaFold DB G0TI59

References