Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN_Sll0205-like-Phd
Location 3118749..3119353 Replicon chromosome
Accession NZ_CP002883
Organism Mycobacterium tuberculosis BT1

Toxin (Protein)


Gene name vapC Uniprot ID P71623
Locus tag HKBT1_RS14855 Protein ID WP_003414492.1
Coordinates 3118749..3119141 (-) Length 131 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A829CBY8
Locus tag HKBT1_RS14860 Protein ID WP_003414495.1
Coordinates 3119138..3119353 (-) Length 72 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HKBT1_RS14825 3113899..3114687 - 789 WP_003917092.1 CRISPR-associated endoribonuclease Cas6 -
HKBT1_RS14830 3115021..3115566 - 546 WP_014584866.1 DUF1802 family protein -
HKBT1_RS14835 3115838..3116722 - 885 WP_003414409.1 nucleotidyl transferase AbiEii/AbiGii toxin family protein -
HKBT1_RS14840 3116725..3117612 - 888 WP_003414414.1 type IV toxin-antitoxin system AbiEi family antitoxin -
HKBT1_RS14845 3117917..3118462 - 546 WP_003910939.1 DUF1802 family protein -
HKBT1_RS14850 3118459..3118728 - 270 WP_003414489.1 DUF2277 family protein -
HKBT1_RS14855 3118749..3119141 - 393 WP_003414492.1 type II toxin-antitoxin system VapC family toxin Toxin
HKBT1_RS14860 3119138..3119353 - 216 WP_003414495.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
HKBT1_RS14865 3119400..3120149 + 750 WP_003902358.1 enoyl-CoA hydratase -
HKBT1_RS14870 3120228..3121310 - 1083 WP_003414499.1 ABC transporter ATP-binding protein -
HKBT1_RS14875 3121303..3122613 - 1311 WP_003414501.1 ABC transporter substrate-binding protein -
HKBT1_RS14880 3122616..3123443 - 828 WP_003414504.1 carbohydrate ABC transporter permease -
HKBT1_RS14885 3123440..3124351 - 912 WP_003414505.1 sugar ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

VapC-like Sll0205 protein, PIN domain

(5-130)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(2-46)


Sequences

Toxin        


Download         Length: 131 a.a.        Molecular weight: 14610.74 Da        Isoelectric Point: 6.7594

>T44767 WP_003414492.1 NZ_CP002883:c3119141-3118749 [Mycobacterium tuberculosis BT1]
MTTVLLDSHVAYWWSAEPQRLSMAASQAIEHADELAVAAISWFELAWLAEQERIQLAIPVLSWLQQLAEHVRTVGITPSV
AATAVALPSSFPGDPADRLIYATAIEHGWRLVTKDRRLRSHRHPRPVTVW

Download         Length: 393 bp

>T44767 NZ_CP002883:c3119141-3118749 [Mycobacterium tuberculosis BT1]
ATGACGACGGTGCTGCTCGACTCGCATGTGGCCTACTGGTGGTCGGCCGAGCCGCAGCGTCTCAGCATGGCGGCGAGCCA
GGCCATCGAACACGCCGACGAGCTCGCCGTCGCCGCGATTTCGTGGTTCGAGCTGGCTTGGCTTGCCGAACAGGAACGCA
TCCAACTGGCGATTCCGGTGCTGTCCTGGCTTCAGCAGCTGGCCGAGCACGTTCGCACCGTCGGTATCACGCCCTCGGTC
GCCGCCACGGCGGTGGCGCTGCCCTCGTCGTTCCCCGGCGATCCGGCCGACCGGTTGATCTACGCCACCGCGATCGAACA
CGGCTGGCGGCTGGTGACCAAGGACCGGCGGCTACGCAGTCATCGGCACCCACGACCGGTCACCGTCTGGTAG

Antitoxin


Download         Length: 72 a.a.        Molecular weight: 7511.55 Da        Isoelectric Point: 4.9732

>AT44767 WP_003414495.1 NZ_CP002883:c3119353-3119138 [Mycobacterium tuberculosis BT1]
MTATEVKAKILSLLDEVAQGEEIEITKHGRTVARLVAATGPHALKGRFSGVAMAAVDDDELFTTGVSWNVS

Download         Length: 216 bp

>AT44767 NZ_CP002883:c3119353-3119138 [Mycobacterium tuberculosis BT1]
ATGACCGCTACGGAGGTGAAGGCGAAGATCCTCTCCTTGCTTGATGAAGTGGCCCAGGGCGAGGAGATCGAGATCACCAA
ACACGGCCGCACCGTGGCCCGGCTGGTGGCAGCGACGGGGCCGCACGCGCTGAAGGGTCGATTCTCGGGTGTGGCGATGG
CGGCCGTGGATGACGACGAACTCTTCACCACCGGGGTTTCGTGGAACGTTTCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4BWM8

Antitoxin

Source ID Structure
AlphaFold DB A0A829CBY8

References