44760

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN(toxin)
Location 2859326..2859963 Replicon chromosome
Accession NZ_CP002883
Organism Mycobacterium tuberculosis BT1

Toxin (Protein)


Gene name vapC Uniprot ID P0DMV7
Locus tag HKBT1_RS13445 Protein ID WP_003413180.1
Coordinates 2859326..2859721 (-) Length 132 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P9WJ44
Locus tag HKBT1_RS13450 Protein ID WP_003413183.1
Coordinates 2859718..2859963 (-) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HKBT1_RS13410 2854730..2855941 + 1212 WP_003900845.1 alpha/beta hydrolase family protein -
HKBT1_RS13415 2856068..2856727 + 660 WP_003902296.1 hypothetical protein -
HKBT1_RS13420 2856724..2857386 + 663 WP_003905878.1 hypothetical protein -
HKBT1_RS22330 2857383..2857660 + 278 Protein_2623 type II toxin-antitoxin system VapB family antitoxin -
HKBT1_RS13425 2857753..2858166 + 414 WP_003413164.1 PIN domain nuclease -
HKBT1_RS13430 2858205..2858462 + 258 WP_003413167.1 ribbon-helix-helix domain-containing protein -
HKBT1_RS13435 2858459..2858836 + 378 WP_003413174.1 type II toxin-antitoxin system VapC family toxin -
HKBT1_RS13440 2858852..2859226 - 375 WP_003413177.1 hypothetical protein -
HKBT1_RS13445 2859326..2859721 - 396 WP_003413180.1 type II toxin-antitoxin system toxin 23S rRNA-specific endonuclease VapC20 Toxin
HKBT1_RS13450 2859718..2859963 - 246 WP_003413183.1 type II toxin-antitoxin system antitoxin VapB20 Antitoxin
HKBT1_RS13455 2860374..2860793 - 420 WP_003413190.1 A24 family peptidase -
HKBT1_RS13460 2860805..2861614 - 810 WP_003413193.1 shikimate dehydrogenase -
HKBT1_RS13465 2861611..2862861 - 1251 WP_024753809.1 endolytic transglycosylase MltG -
HKBT1_RS13470 2862854..2863366 - 513 WP_003413197.1 Holliday junction resolvase RuvX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Endonuclease VapC20-like

(1-129)

Antitoxin

Ribbon-helix-helix protein, CopG

(11-49)


Sequences

Toxin        


Download         Length: 132 a.a.        Molecular weight: 14619.53 Da        Isoelectric Point: 6.9794

>T44760 WP_003413180.1 NZ_CP002883:c2859721-2859326 [Mycobacterium tuberculosis BT1]
MIFVDTSFWAALGNAGDARHGTAKRLWASKPPVVMTSNHVLGETWTLLNRRCGHRAAVAAAAIRLSTVVRVEHVTADLEE
QAWEWLVRHDEREYSFVDATSFAVMRKKGIQNAYAFDGDFSAAGFVEVRPE

Download         Length: 396 bp

>T44760 NZ_CP002883:c2859721-2859326 [Mycobacterium tuberculosis BT1]
ATGATCTTCGTCGACACGTCCTTCTGGGCTGCACTTGGCAACGCCGGCGACGCTCGGCACGGCACCGCGAAGAGGTTGTG
GGCCAGCAAACCGCCCGTGGTGATGACCTCCAACCATGTCCTGGGCGAGACCTGGACGCTGCTCAACCGGCGCTGCGGTC
ACCGCGCGGCGGTTGCCGCCGCTGCAATCCGCTTGAGCACCGTCGTTCGCGTCGAGCACGTAACAGCCGACCTGGAAGAG
CAGGCATGGGAATGGCTTGTACGCCACGACGAGCGAGAGTACTCGTTCGTCGATGCCACGAGCTTCGCGGTGATGCGAAA
GAAAGGCATCCAAAATGCGTATGCCTTCGATGGTGACTTCAGCGCGGCCGGATTTGTCGAGGTAAGACCCGAGTAG

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 9098.33 Da        Isoelectric Point: 5.6007

>AT44760 WP_003413183.1 NZ_CP002883:c2859963-2859718 [Mycobacterium tuberculosis BT1]
MLVAYICHVKRLQIYIDEDVDRALAVEARRRRTSKAALIREYVAEHLRQPGPDPVDAFVGSFVGEADLSASVDDVVYGKH
E

Download         Length: 246 bp

>AT44760 NZ_CP002883:c2859963-2859718 [Mycobacterium tuberculosis BT1]
ATGCTAGTGGCGTATATTTGCCATGTGAAAAGGCTGCAGATCTACATCGACGAAGACGTTGACCGAGCGCTTGCTGTTGA
AGCGCGACGGCGGCGGACGTCGAAGGCGGCGCTGATCCGGGAGTACGTCGCTGAGCACCTCCGGCAGCCGGGCCCAGATC
CGGTCGATGCCTTCGTCGGATCGTTCGTGGGGGAAGCCGACTTGTCCGCGTCCGTCGACGACGTGGTTTACGGAAAGCAC
GAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5WZ4
PDB 5WZF

Antitoxin

Source ID Structure
AlphaFold DB A0A7U4BW31

References