Detailed information of TA system TA044759

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 2858205..2858836
• Replicon: chromosome
• Accession: NZ_CP002883
• Organism: Mycobacterium tuberculosis BT1

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TQ28
• Locus tag: HKBT1_RS13435
• Protein ID: WP_003413174.1
• Coordinates: 2858459..2858836 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: P95006
• Locus tag: HKBT1_RS13430
• Protein ID: WP_003413167.1
• Coordinates: 2858205..2858462 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HKBT1_RS13405	2854120..2854434	+	315	WP_009937839.1	hypothetical protein	-
HKBT1_RS13410	2854730..2855941	+	1212	WP_003900845.1	alpha/beta hydrolase family protein	-
HKBT1_RS13415	2856068..2856727	+	660	WP_003902296.1	hypothetical protein	-
HKBT1_RS13420	2856724..2857386	+	663	WP_003905878.1	hypothetical protein	-
HKBT1_RS22330	2857383..2857660	+	278	Protein_2623	type II toxin-antitoxin system VapB family antitoxin	-
HKBT1_RS13425	2857753..2858166	+	414	WP_003413164.1	PIN domain nuclease	-
HKBT1_RS13430	2858205..2858462	+	258	WP_003413167.1	ribbon-helix-helix domain-containing protein	Antitoxin
HKBT1_RS13435	2858459..2858836	+	378	WP_003413174.1	type II toxin-antitoxin system VapC family toxin	Toxin
HKBT1_RS13440	2858852..2859226	-	375	WP_003413177.1	hypothetical protein	-
HKBT1_RS13445	2859326..2859721	-	396	WP_003413180.1	type II toxin-antitoxin system toxin 23S rRNA-specific endonuclease VapC20	-
HKBT1_RS13450	2859718..2859963	-	246	WP_003413183.1	type II toxin-antitoxin system antitoxin VapB20	-
HKBT1_RS13455	2860374..2860793	-	420	WP_003413190.1	A24 family peptidase	-
HKBT1_RS13460	2860805..2861614	-	810	WP_003413193.1	shikimate dehydrogenase	-
HKBT1_RS13465	2861611..2862861	-	1251	WP_024753809.1	endolytic transglycosylase MltG	-
HKBT1_RS13470	2862854..2863366	-	513	WP_003413197.1	Holliday junction resolvase RuvX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (3-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (2-38)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13728.72 Da
• Isoelectric Point: 4.4687

>T44759 WP_003413174.1 NZ_CP002883:2858459-2858836 [Mycobacterium tuberculosis BT1]
VKLIDTTIAVDHLRGEPAAAVLLAELINNGEEIAASELVRFELLAGVRESELAALEAFFSAVVWTLVTEDIARIGGRLAR
RYRSSHRGIDDVDYLIAATAIVVDADLLTTNVRHFPMFPDLQPPY

Nucleotide

• Length: 378 bp

>T44759 NZ_CP002883:2858459-2858836 [Mycobacterium tuberculosis BT1]
GTGAAGCTGATCGACACCACCATCGCGGTCGACCACCTTCGCGGCGAACCCGCGGCAGCCGTGCTGCTCGCCGAACTGAT
AAACAACGGTGAGGAGATCGCGGCCAGCGAGCTGGTCCGATTCGAACTCCTCGCCGGTGTGCGGGAAAGCGAACTCGCGG
CGCTCGAGGCCTTCTTCTCGGCAGTGGTGTGGACCCTGGTGACCGAGGACATTGCCCGGATCGGCGGACGACTCGCCCGT
CGATACCGGTCCAGCCACCGCGGTATCGACGACGTGGACTACCTGATCGCTGCGACCGCCATTGTGGTCGACGCCGACCT
GCTCACCACCAATGTGCGCCACTTCCCGATGTTCCCGGATCTGCAGCCGCCGTACTGA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9453.63 Da
• Isoelectric Point: 10.6555

>AT44759 WP_003413167.1 NZ_CP002883:2858205-2858462 [Mycobacterium tuberculosis BT1]
MRTQVTLGKEELELLDRAAKASGASRSELIRRAIHRAYGTGSKQERLAALDHSRGSWRGRDFTGTEYVDAIRGDLNERLA
RLGLA

Nucleotide

• Length: 258 bp

>AT44759 NZ_CP002883:2858205-2858462 [Mycobacterium tuberculosis BT1]
ATGCGGACTCAGGTGACCCTGGGCAAAGAGGAGCTTGAGCTGCTCGATCGTGCCGCCAAGGCGAGTGGCGCATCGCGGTC
CGAACTCATCCGACGCGCAATTCACCGTGCCTACGGGACTGGATCCAAGCAGGAACGGCTCGCCGCGCTCGACCACAGCC
GTGGCTCGTGGCGAGGACGGGACTTCACCGGCACCGAGTATGTCGACGCCATTCGGGGCGACCTCAACGAACGACTTGCT
CGGCTCGGTCTGGCGTGA

Structures

Toxin

• AlphaFold DB: G0TQ28

Antitoxin

• AlphaFold DB: A0A829C9W5