Detailed information of TA system TA044751

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 2248417..2249043
• Replicon: chromosome
• Accession: NZ_CP002883
• Organism: Mycobacterium tuberculosis BT1

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P64926
• Locus tag: HKBT1_RS10535
• Protein ID: WP_003410075.1
• Coordinates: 2248645..2249043 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A806JLL8
• Locus tag: HKBT1_RS10530
• Protein ID: WP_003911750.1
• Coordinates: 2248417..2248644 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HKBT1_RS10520	2246456..2246800	-	345	WP_003410065.1	ferredoxin family protein	-
HKBT1_RS10525	2246989..2248158	-	1170	WP_003899126.1	ATP-binding protein	-
HKBT1_RS10530	2248417..2248644	+	228	WP_003911750.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
HKBT1_RS10535	2248645..2249043	+	399	WP_003410075.1	PIN domain nuclease	Toxin
HKBT1_RS10540	2249226..2249657	-	432	WP_003410078.1	MarR family winged helix-turn-helix transcriptional regulator	-
HKBT1_RS10545	2249758..2250192	+	435	WP_003914358.1	DUF1398 domain-containing protein	-
HKBT1_RS21390	2250629..2250808	-	180	Protein_2069	hypothetical protein	-
HKBT1_RS10550	2250869..2251516	+	648	WP_078378032.1	transposase	-
HKBT1_RS10555	2251470..2252060	+	591	WP_003899131.1	IS110 family transposase	-
HKBT1_RS10560	2252188..2253444	-	1257	WP_003902247.1	HNH endonuclease	-
HKBT1_RS10565	2253798..2253998	+	201	Protein_2073	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-120)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (2-43)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14731.02 Da
• Isoelectric Point: 6.7067

>T44751 WP_003410075.1 NZ_CP002883:2248645-2249043 [Mycobacterium tuberculosis BT1]
MIVDTSVWIAYLSTSESLASRWLADRIAADSTVIVPEVVMMELLIGKTDEDTAALRRRLLQRFAIEPLAPVRDAEDAAAI
HRRCRRGGDTVRSLIDCQVAAMALRIGVAVAHRDRDYEAIRTHCGLRTEPLF

Nucleotide

• Length: 399 bp

>T44751 NZ_CP002883:2248645-2249043 [Mycobacterium tuberculosis BT1]
ATGATCGTCGACACCTCGGTCTGGATCGCATATCTCTCCACGTCAGAGTCGTTGGCCAGTCGCTGGCTAGCCGATCGCAT
TGCCGCTGACTCGACGGTGATCGTGCCCGAGGTGGTGATGATGGAGCTGCTGATCGGTAAGACCGATGAGGACACCGCCG
CACTGCGCCGACGGCTCCTGCAGCGATTCGCTATCGAACCGCTGGCCCCGGTCCGCGACGCGGAAGATGCCGCCGCCATT
CACCGGCGCTGTCGTCGCGGCGGCGACACCGTACGCAGCCTGATCGATTGCCAGGTGGCCGCGATGGCGTTGCGGATCGG
GGTCGCCGTGGCGCATCGTGATCGCGACTACGAGGCGATCCGCACACATTGCGGACTACGCACCGAGCCGTTGTTCTGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8346.16 Da
• Isoelectric Point: 4.1621

>AT44751 WP_003911750.1 NZ_CP002883:2248417-2248644 [Mycobacterium tuberculosis BT1]
VSRTNIEIDDELVAAAQRMYRLDSKRSAVDLALRRLVGEPLGRDEALALQGSGFDFSNDEIESFSDTDRKLADES

Nucleotide

• Length: 228 bp

>AT44751 NZ_CP002883:2248417-2248644 [Mycobacterium tuberculosis BT1]
GTGTCACGGACCAACATCGAGATCGACGACGAACTCGTGGCCGCCGCACAGCGGATGTACCGACTCGATTCCAAGCGAAG
TGCCGTCGACCTCGCGCTGCGCCGGCTCGTGGGTGAACCGTTGGGCCGCGATGAGGCTTTGGCGCTGCAGGGCAGCGGTT
TCGACTTCAGCAACGATGAGATCGAATCGTTCTCGGATACGGACCGCAAGCTCGCCGACGAGTCGTAG

Structures

Toxin

• PDB: 4CHG

Antitoxin

• AlphaFold DB: A0A806JLL8