Detailed information of TA system TA044745

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2180520..2181223
• Replicon: chromosome
• Accession: NZ_CP002883
• Organism: Mycobacterium tuberculosis BT1

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G0TLK7
• Locus tag: HKBT1_RS10115
• Protein ID: WP_003409778.1
• Coordinates: 2180520..2180849 (-)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: G0TLK8
• Locus tag: HKBT1_RS21355
• Protein ID: WP_003409780.1
• Coordinates: 2180846..2181223 (-)
• Length: 126 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HKBT1_RS10095	2176903..2177973	+	1071	WP_003902252.1	epoxide hydrolase EphB	-
HKBT1_RS10100	2177970..2178485	+	516	WP_003409718.1	flavin reductase family protein	-
HKBT1_RS10105	2178482..2179543	+	1062	WP_003899092.1	3,4-dihydroxy-2-butanone-4-phosphate synthase	-
HKBT1_RS10110	2179540..2180310	+	771	WP_003409775.1	SDR family oxidoreductase	-
HKBT1_RS10115	2180520..2180849	-	330	WP_003409778.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
HKBT1_RS21355	2180846..2181223	-	378	WP_003409780.1	type II toxin-antitoxin system antitoxin MazE5	Antitoxin
HKBT1_RS10125	2181220..2181810	-	591	WP_003409784.1	SEC-C domain-containing protein	-
HKBT1_RS10130	2181865..2183229	+	1365	WP_003903691.1	HNH endonuclease	-
HKBT1_RS10135	2183384..2183836	-	453	WP_003899095.1	lipoprotein	-
HKBT1_RS10140	2183900..2184301	+	402	WP_003409869.1	hypothetical protein	-
HKBT1_RS10145	2184294..2184476	-	183	WP_003409870.1	hypothetical protein	-
HKBT1_RS10150	2184590..2184940	-	351	WP_003409871.1	hypothetical protein	-
HKBT1_RS10155	2184951..2185853	-	903	WP_003899097.1	hypothetical protein	-
HKBT1_RS10160	2185874..2186065	-	192	WP_003409876.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-103)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 11787.82 Da
• Isoelectric Point: 8.5361

>T44745 WP_003409778.1 NZ_CP002883:c2180849-2180520 [Mycobacterium tuberculosis BT1]
VTALPARGEVWWCEMAEIGRRPVVVLSRDAAIPRLRRALVAPCTTTIRGLASEVVLEPGSDPIPRRSAVNLDSVESVSVA
VLVNRLGRLADIRMRAICTALEVAVDCSR

Nucleotide

• Length: 330 bp

>T44745 NZ_CP002883:c2180849-2180520 [Mycobacterium tuberculosis BT1]
GTGACCGCACTTCCGGCGCGCGGAGAGGTGTGGTGGTGTGAGATGGCTGAGATCGGTCGGCGACCAGTCGTCGTGCTGTC
GCGCGATGCCGCGATCCCTCGGCTGCGACGCGCACTTGTCGCGCCCTGCACCACGACCATCCGAGGGCTAGCCAGTGAGG
TTGTTCTTGAACCCGGTTCCGACCCGATCCCGCGCCGTTCCGCGGTGAATTTGGACTCAGTCGAAAGTGTCTCGGTCGCG
GTATTGGTGAATCGGCTTGGCCGCCTCGCCGACATCCGGATGCGCGCCATCTGCACGGCCCTCGAGGTCGCCGTCGATTG
CTCTCGATGA

Antitoxin

Protein

• Length: 126 a.a.
• Molecular weight: 13560.07 Da
• Isoelectric Point: 5.1519

>AT44745 WP_003409780.1 NZ_CP002883:c2181223-2180846 [Mycobacterium tuberculosis BT1]
VKTARLQVTLRCAVDLINSSSDQCFARIEHVASDQADPRPGVWHSSGMNRIRLSTTVDAALLTSARDMRAGITDAALIDE
ALAALLARHRSAEVDASYAAYDKHPVDEPDEWGDLASWRRAAGDS

Nucleotide

• Length: 378 bp

>AT44745 NZ_CP002883:c2181223-2180846 [Mycobacterium tuberculosis BT1]
GTGAAGACGGCCCGGTTGCAGGTGACGTTGCGCTGCGCGGTAGACCTCATCAATTCGTCCAGCGACCAATGTTTCGCCCG
TATCGAGCACGTTGCGTCCGATCAGGCGGATCCACGCCCCGGAGTGTGGCATAGTAGTGGCATGAATCGCATACGGCTGA
GCACTACTGTCGACGCCGCACTCTTGACAAGTGCGCGCGACATGCGGGCAGGAATCACTGACGCAGCCCTCATCGATGAG
GCCCTTGCGGCATTACTGGCTCGTCATCGATCGGCCGAGGTGGATGCCAGCTATGCGGCCTACGACAAGCACCCGGTCGA
TGAGCCCGACGAGTGGGGCGATCTAGCGTCGTGGCGGCGGGCGGCTGGAGACTCGTGA

Structures

Toxin

• AlphaFold DB: G0TLK7

Antitoxin

• AlphaFold DB: G0TLK8