Detailed information of TA system TA044736

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 1236991..1237559
• Replicon: chromosome
• Accession: NZ_CP002883
• Organism: Mycobacterium tuberculosis BT1

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TH08
• Locus tag: HKBT1_RS05895
• Protein ID: WP_003405865.1
• Coordinates: 1237185..1237559 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TH07
• Locus tag: HKBT1_RS05890
• Protein ID: WP_003405863.1
• Coordinates: 1236991..1237188 (+)
• Length: 66 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HKBT1_RS05870	1233032..1233670	-	639	WP_003898730.1	lipid droplet-associated protein	-
HKBT1_RS05875	1233760..1234767	+	1008	WP_003405853.1	4-hydroxy-3-methylbut-2-enyl diphosphate reductase	-
HKBT1_RS05880	1234784..1235767	-	984	WP_003898731.1	hypothetical protein	-
HKBT1_RS05885	1235830..1236903	+	1074	WP_003898732.1	redox-regulated ATPase YchF	-
HKBT1_RS05890	1236991..1237188	+	198	WP_003405863.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
HKBT1_RS05895	1237185..1237559	+	375	WP_003405865.1	type II toxin-antitoxin system VapC family toxin	Toxin
HKBT1_RS05900	1237762..1238460	+	699	WP_003898733.1	hypothetical protein	-
HKBT1_RS05905	1238578..1238763	+	186	WP_003901093.1	hypothetical protein	-
HKBT1_RS05910	1238690..1238965	-	276	WP_003405867.1	hypothetical protein	-
HKBT1_RS05915	1239208..1239531	+	324	WP_003405871.1	antibiotic biosynthesis monooxygenase	-
HKBT1_RS05920	1239546..1240256	-	711	WP_003911399.1	hypothetical protein	-
HKBT1_RS22565	1240278..1240487	+	210	WP_003911400.1	hypothetical protein	-
HKBT1_RS05930	1240439..1241146	-	708	Protein_1158	adenylate/guanylate cyclase domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-111)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-42)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13444.56 Da
• Isoelectric Point: 5.1881

>T44736 WP_003405865.1 NZ_CP002883:1237185-1237559 [Mycobacterium tuberculosis BT1]
VILVDTSVWIEHLRAADARLVELLGDDEAGCHPLVIEELALGSIKQRDVVLDLLANLYQFPVVTHDEVLRLVGRRRLWGR
GLGAVDANLLGSVALVGGARLWTRDKRLKAACAESGVALAEEVS

Nucleotide

• Length: 375 bp

>T44736 NZ_CP002883:1237185-1237559 [Mycobacterium tuberculosis BT1]
GTGATCCTGGTCGACACTTCGGTATGGATTGAGCACCTGCGCGCCGCCGACGCGCGACTCGTCGAGCTGCTGGGCGATGA
CGAGGCCGGTTGCCATCCGCTCGTCATCGAGGAGCTGGCGCTTGGCTCGATCAAGCAGCGAGACGTTGTTCTCGATCTGT
TGGCCAACCTCTACCAGTTTCCGGTGGTGACCCACGACGAAGTGTTGCGGCTTGTCGGTCGGCGGCGGTTGTGGGGTCGG
GGACTCGGTGCCGTCGATGCCAACCTTCTTGGTTCGGTGGCTCTGGTTGGCGGCGCGCGACTATGGACGCGGGACAAGCG
GTTGAAGGCGGCGTGCGCGGAAAGCGGTGTTGCGCTGGCTGAGGAAGTGTCCTGA

Antitoxin

Protein

• Length: 66 a.a.
• Molecular weight: 6973.03 Da
• Isoelectric Point: 11.7327

>AT44736 WP_003405863.1 NZ_CP002883:1236991-1237188 [Mycobacterium tuberculosis BT1]
MRTTVTVDDALLAKAAELTGVKEKSTLLREGLQTLVRVESARRLAALGGTDPQATAAPRRRTSPR

Nucleotide

• Length: 198 bp

>AT44736 NZ_CP002883:1236991-1237188 [Mycobacterium tuberculosis BT1]
ATGAGGACGACGGTGACCGTTGACGACGCCTTGTTAGCCAAAGCGGCCGAATTGACTGGGGTGAAAGAGAAGTCGACGCT
CCTGCGCGAGGGGTTGCAGACACTGGTCCGGGTGGAGAGCGCCCGGCGGTTGGCGGCTCTCGGCGGCACCGACCCGCAAG
CTACCGCGGCGCCGAGACGCCGGACGTCGCCCCGGTGA

Structures

Toxin

• AlphaFold DB: G0TH08

Antitoxin

• AlphaFold DB: G0TH07