Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 1228235..1228866 Replicon chromosome
Accession NZ_CP002883
Organism Mycobacterium tuberculosis BT1

Toxin (Protein)


Gene name mazF Uniprot ID A0A7U4F9D0
Locus tag HKBT1_RS05835 Protein ID WP_003405820.1
Coordinates 1228235..1228546 (-) Length 104 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G0TGZ7
Locus tag HKBT1_RS05840 Protein ID WP_003405836.1
Coordinates 1228546..1228866 (-) Length 107 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HKBT1_RS05815 1223716..1225140 - 1425 WP_003405805.1 class II fumarate hydratase -
HKBT1_RS05820 1225171..1226259 - 1089 WP_003898726.1 class II fructose-bisphosphatase -
HKBT1_RS05825 1226315..1226959 + 645 WP_003915494.1 DUF4245 domain-containing protein -
HKBT1_RS05830 1226966..1228123 - 1158 WP_003898727.1 AI-2E family transporter -
HKBT1_RS05835 1228235..1228546 - 312 WP_003405820.1 type II toxin-antitoxin system toxin endoribonuclease MazF3 Toxin
HKBT1_RS05840 1228546..1228866 - 321 WP_003405836.1 type II toxin-antitoxin system antitoxin MazE3 Antitoxin
HKBT1_RS21070 1228876..1230401 + 1526 Protein_1142 carboxylesterase/lipase family protein -
HKBT1_RS05855 1230419..1231531 - 1113 WP_003405840.1 3 beta-hydroxysteroid dehydrogenase/delta 5-->4-isomerase -
HKBT1_RS05860 1231541..1231798 - 258 WP_003405844.1 exodeoxyribonuclease VII small subunit -
HKBT1_RS05865 1231788..1233035 - 1248 WP_003405846.1 exodeoxyribonuclease VII large subunit -
HKBT1_RS05870 1233032..1233670 - 639 WP_003898730.1 lipid droplet-associated protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(8-85)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 11061.82 Da        Isoelectric Point: 4.9371

>T44735 WP_003405820.1 NZ_CP002883:c1228546-1228235 [Mycobacterium tuberculosis BT1]
MRPIHIAQLDKARPVLILTREVVRPHLTNVTVAPITTTVRGLATEVPVDAVNGLNQPSVVSCDNIQTIPVCDLGRQIGYL
LASQEPALAEAIGNAFDLDWVVA

Download         Length: 312 bp

>T44735 NZ_CP002883:c1228546-1228235 [Mycobacterium tuberculosis BT1]
ATGCGACCTATCCACATCGCACAGCTTGACAAGGCTAGACCCGTCCTGATCCTTACCCGCGAGGTCGTACGTCCGCACCT
CACGAACGTCACCGTCGCCCCGATCACGACAACCGTGCGTGGGCTAGCCACCGAAGTCCCGGTAGACGCGGTGAACGGGC
TGAATCAACCGTCAGTCGTCAGCTGCGACAACATCCAAACGATCCCGGTGTGTGACCTTGGTCGTCAAATCGGCTACCTG
CTCGCGTCGCAGGAGCCGGCTCTAGCCGAGGCCATCGGCAACGCGTTCGACCTCGATTGGGTGGTGGCATAG

Antitoxin


Download         Length: 107 a.a.        Molecular weight: 11394.83 Da        Isoelectric Point: 5.1236

>AT44735 WP_003405836.1 NZ_CP002883:c1228866-1228546 [Mycobacterium tuberculosis BT1]
MYLPWGVVLAGGANGFGAGAYQTGTICEVSTQIAVRLPDEIVAFIDDEVRGQHARSRAAVVLRALERERRRRLAERDAEI
LATNTSATGDLDTLAGHCARTALDID

Download         Length: 321 bp

>AT44735 NZ_CP002883:c1228866-1228546 [Mycobacterium tuberculosis BT1]
ATGTACCTACCCTGGGGGGTCGTCCTCGCCGGCGGCGCGAACGGTTTTGGGGCGGGTGCATACCAAACTGGTACCATCTG
TGAAGTGAGTACACAGATTGCGGTTCGCTTGCCCGATGAGATTGTCGCGTTCATCGACGACGAAGTCCGGGGACAGCATG
CCCGTAGCCGTGCGGCTGTCGTGCTGCGGGCGCTGGAACGGGAACGCCGCCGTCGTCTCGCCGAACGCGACGCGGAGATC
CTCGCCACCAACACGTCAGCCACGGGTGATCTGGATACGCTGGCCGGCCACTGCGCTCGTACCGCCCTCGACATCGACTG
A

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4F9D0

Antitoxin

Source ID Structure
AlphaFold DB G0TGZ7

References