Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1-PHD
Location 693399..694045 Replicon chromosome
Accession NZ_CP002883
Organism Mycobacterium tuberculosis BT1

Toxin (Protein)


Gene name vapC Uniprot ID O07783
Locus tag HKBT1_RS03090 Protein ID WP_003403122.1
Coordinates 693399..693791 (-) Length 131 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID G0TQ86
Locus tag HKBT1_RS03095 Protein ID WP_003403125.1
Coordinates 693788..694045 (-) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HKBT1_RS03075 689061..690587 + 1527 WP_003403112.1 virulence factor Mce family protein -
HKBT1_RS03080 690650..691792 + 1143 WP_003911253.1 virulence factor Mce family protein -
HKBT1_RS03085 691797..693347 + 1551 WP_003905349.1 MCE family protein -
HKBT1_RS03090 693399..693791 - 393 WP_003403122.1 type II toxin-antitoxin system toxin ribonuclease C4 Toxin
HKBT1_RS03095 693788..694045 - 258 WP_003403125.1 type II toxin-antitoxin system antitoxin VapB4 Antitoxin
HKBT1_RS03100 694228..695463 - 1236 WP_003403128.1 ATP-binding protein -
HKBT1_RS03105 695714..696127 - 414 WP_003403137.1 type II toxin-antitoxin system VapC family toxin -
HKBT1_RS03110 696124..696360 - 237 WP_003403139.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
HKBT1_RS03115 696464..696970 - 507 WP_003900973.1 two component system sensor kinase HK1 -
HKBT1_RS03120 697084..697614 - 531 WP_003917320.1 two component system sensor kinase HK2 -
HKBT1_RS03125 697598..698359 - 762 WP_003403148.1 two-component system response regulator TcrA -
HKBT1_RS03130 698416..698727 + 312 WP_003403164.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(8-122)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(3-53)


Sequences

Toxin        


Download         Length: 131 a.a.        Molecular weight: 14080.08 Da        Isoelectric Point: 4.6558

>T44722 WP_003403122.1 NZ_CP002883:c693791-693399 [Mycobacterium tuberculosis BT1]
VNVRRALADTSVFIGIEATRFDPDRFAGYEWGVSVVTLGELRLGVLQASGPEAAARRLSTYQLAQRFEPLGIDEAVSEAW
ALLVSKLRAAKLRVPINDSWIAATAVAHGIAILTQDNDYAAMPDVEVITI

Download         Length: 393 bp

>T44722 NZ_CP002883:c693791-693399 [Mycobacterium tuberculosis BT1]
GTGAACGTACGGCGCGCCCTCGCCGACACATCCGTTTTCATTGGCATCGAGGCGACACGATTCGACCCGGACCGGTTTGC
GGGATACGAATGGGGCGTCTCGGTAGTCACACTCGGCGAGCTGCGGCTCGGGGTGTTGCAAGCCTCCGGCCCCGAGGCCG
CCGCACGTCGGCTCTCCACCTACCAGCTTGCGCAACGGTTCGAACCACTAGGCATCGACGAGGCGGTCTCCGAAGCATGG
GCATTACTGGTGTCCAAGCTGCGCGCCGCCAAACTGCGCGTGCCGATCAACGACAGCTGGATTGCGGCCACAGCCGTGGC
GCACGGCATTGCGATCCTGACCCAAGACAACGACTACGCCGCCATGCCCGACGTCGAGGTCATAACGATCTGA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9681.06 Da        Isoelectric Point: 7.0175

>AT44722 WP_003403125.1 NZ_CP002883:c694045-693788 [Mycobacterium tuberculosis BT1]
MSATIPARDLRNHTAEVLRRVAAGEEIEVLKDNRPVARIVPLKRRRQWLPAAEVIGELVRLGPDTTNLGEELRETLTQTT
DDVRW

Download         Length: 258 bp

>AT44722 NZ_CP002883:c694045-693788 [Mycobacterium tuberculosis BT1]
ATGTCTGCTACGATACCCGCTCGCGATCTTCGCAACCACACCGCCGAGGTACTGCGGCGAGTTGCCGCCGGCGAGGAAAT
CGAGGTGCTCAAGGACAATCGCCCCGTAGCGCGCATCGTTCCGCTCAAGCGGCGCCGCCAATGGTTGCCAGCTGCCGAGG
TGATCGGCGAACTGGTGCGCTTGGGCCCCGATACCACCAATCTGGGCGAGGAGCTGCGAGAGACGCTGACGCAAACCACG
GACGATGTGCGGTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4F8V4

Antitoxin

Source ID Structure
AlphaFold DB G0TQ86

References